Team:DTU-Denmark/Notebook/17 September 2013

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Contents

Description

The purpose of this experiment is to determine the long term characteristics of the growth of our Nir transformant anerobically. Specifically, we measure nitrous oxide production over a period of 10 hours.

Methods

Protocol Bioreactor

This image shows the bioreactor set up to run in triplicate.

Results

Nitrous Oxide measured in the bioreactor at the beginning and end of the experiment.

Time Replicate 1 Replicate 2 Replicate 3 Control: Distilled Water
14:00 1.84 mg/L 1.64 mg/L 1.60 mg/L N/A
22:00 1.43 mg/L 1.44 mg/L 1.50 mg/L 1.65 mg/L

Discussion

There were several sources of error in this experiment. Most importantly, the experiment became aerobic in each chamber at 12:00, and the lab technician began sparging the reactors.

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