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Team:Groningen/Modeling/Heatmotility
From 2013.igem.org
Contents |
Heat Motility
The goal for our heat motility system is to obtain higher concentrations of silk in close proximity to the implant. This is achieved by immobilizing the silk producing bacteria once they are within some distance to the implant, which is enabled by integrating the the standard chemotaxis system with the DesK membrane fluidity sensor system. The result is that B. Subtilis stops moving when it is 37°C, and swims when it is 25°C. In our model we simulate the behaviour of these systems and, if necessary, implement biologically plausible modifications to make it feasible.
The chemotaxis system of B. subtilis
The CheV subsystem
The methylation subsystem
The CheC CheD subsystem
Exact adaption
Motility model
Homogenous and non-homogenous environments
CheC knockout
The effects of temperature
The DesK system
Demonstration
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