Team:DTU-Denmark/Notebook/11 September 2013

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11 September

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Contents

lab 208


Main purpose


Who was in the lab


Henrike

Procedure


Colony PCR to verify Nir in pZA21::ara-tight

Colony PCR with sequencing primers Pair 1: Nir_FW_4_Seq + Nir_RV_5_Seq . Pair 2: Nir_FW_13_Seq + Nir_RV_14_Seq.

Re-run of yesterday's PCR with the following new annealing temperatures: pair 1: 67C, pair 2: 68C

Results


Gels

  • 1 kb
  • colony 1, HAO
  • colony 2, HAO
  • colony 3, HAO
  • colony 4, HAO
  • colony 5, HAO
  • colony 6, HAO
  • Nir colony 7, primer pair 1
  • Nir colony 7, primer pair 2
  • Nir colony 8, primer pair 1
  • Nir colony 8, primer pair 2
  • 1 kb

2013-09-11 colhao colnir seqprimer2.jpg

Made new PCR with higher annealing temperature to obliterate unspecific binding.

  • 1 kb
  • Nir colony 1, primer pair 1
  • Nir colony 1, primer pair 1
  • Nir colony 1, primer pair 2
  • Nir colony 1, primer pair 2

2013-09-11 nir col seqprimr highertemp.jpg

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