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Team:Wageningen UR/Collaborations
From 2013.igem.org
- Safety introduction
- General safety
- Fungi-related safety
- Biosafety Regulation
- Safety Improvement Suggestions
- Safety of the Application
- Masterclass
- Science cafe
- iGEM Netherlands
- Collaborations
- Conference
- Vitruvian man
- Masterclass
- Science cafe
- iGEM Netherlands
- Collaborations
- Conference
- Vitruvian man
iGEM team Cornell University
The iGEM team of Cornell is one of the iGEM teams that is, as well as we are, working on a fungus. They are working on basidiomycetes and are making a toolkit for this unknown chassis. For the collaboration between iGEM Cornell and us we exchanged biobricks. This would be a pki promoter and terminator from our site and we got a cre-recombinase and two resistance genes (geneticin and hygomycin) from them to be tested in A. niger. For the geneticin resistance markers A. niger was transformed with the construct and at different geneticin the growth of a negative control and the transformants are checked. It appeared that 60µg/mL is not enough geneticin to inhibit the growth of A. niger without the resistance gene. Another growth experience was done with a concentration of 220µg/mL.
Samples for NRP UEA Norwich
The iGEM team of NRP UEA Norwich is working on a biosensor to be able to detect antimycins, an anti-fungal compound produced by Streptomyces. For the quality of their project they asked different iGEM teams to send them soil samples from different places in their hometown. Of course we grabbed our bikes, played in the mud and collected samples to help out the iGEM team of Norwich.
Soil samples in Greiner tubes
Protocols for TU Delft iGEM team
Gel extraction and DNA purification kits are expensive to come by for most iGEM teams. Few of our team members found a cheaper and effective alternative for the same. These are homemade silica spin columns which have made it into the Elseiver journal, Analytical biochemistry. We sent this protocol to the iGEM team of TU Delft as they had problems with gel extraction and buying expensive kits.
Questionnaire for iGEM Purdue
The iGEM team of Purdue asked iGEM teams to fill in a questionnaire about the standardization of the data sheet of the biobricks. They asked more than 50 teams around the world about their opinion and iGEM Wageningen was one of them. Of course we were happy to fill in this questionnaire and help them. Especially because it will help us and future iGEM teams in submitting their parts and search for other interesting parts.
Survey about your peronal motivation from iGEM Paris
Why are you joining the iGEM and what experiences do you want to get and actually get? This is something the iGEM team Paris was wondering about and that is why they sent around a survey we were happy to fill in. Every team member was asked to do it separately as it was a survey about your motivation.
