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From 2013.igem.org
Revision as of 14:37, 19 July 2013 by
Ddesai
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Our Team
Team
Summer Fun
Project
Project Overview and Abstract
MoClo DNA Assembly
Quorum Sensing
Datasheet App
Clotho
Results Summary
Achievements
Data Collected
Parts Submitted
MoClo Kit
Medal Fulfillment
Notebook
Protocols
Characterization Notebook
Quorum Sensing Notebook
Software Notebook
Troubleshooting
Considerations
New England iGEM Regional Meeting
Human Practices and Safety Forms
Acknowledgements
Collaborations
Acknowledgements
Protocols
This section contains the protocols that we used during the summer and that are mentioned in our Notebook. We hope they help anyone interested in our experiments to successfully repeat them.
DNA Quantification
Gel Extraction
Miniprep
PCR Cleanup
Ligation
Media Prep
MoClo Level 0 Reaction
MoClo Level 1 and 2 Reactions
Phusion PCR
Restriction Digest
Restriction Digest 10 µL mapping
Transformation
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