Lab 115

Main purpose

Who was in the lab

Helen, Kasia

Procedure

Results

Conclusion

Lab 208

Main purpose

• miniprep of TAT, Sec, and Nir for sequencing
• LB medium preparation (2L)
• Pick up new PAO1 cultures
• Make new colony PCR to get Nir from the newly acquired colonies.

Who was in the lab

Kristian, Julia

Procedure

PCRs:

• pZA21::RFP with primer pair 13 58C and 3:00.
• araBAD from K808000 with primer pair 12 61C and 1:00
• Colony PCR with first PAO1 colony but with a gradient of concentrations from 1x to 500x
• Colony PCR with the 4 new plates of PAO1, concentration x1

Results

See tomorrows gel.

Conclusion

Nothing to conclude from todays work. Navigate to the Previous or the Next Entry