Team:Bielefeld-Germany/Labjournal/June

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June

Milesstones


1.Week

MFC

Mediators

Cytochromes

Biosafety

Porines









2.Week

MFC

Mediators

Cytochromes

Biosafety

Porines









3.Week

MFC

Mediators

Cytochromes

Cultivation of Shewanella oneidensis MR-1 in liquid LB medium at 30 °C

Isolation of genomic DNA from S. oneidensis


4-2006-451: Conc.:453.1 ng/µl    OD260/280: 1.92    OD260/230:2.78
4-2006-452: Conc.:447.1 ng/µl    OD260/280: 1.92    OD260/230:2.67

Dilution to PCR template: 4-2006-453: 5.5ng/µl

Amplification of the mtrCAB cluster

Protocol: Phusion 50µl Gradient
Gradient: 55.8-56.7-57.8-59.1-60.4-61.7-62.9-63.9
Clear Bands at the expected 5.2kb

PCR-CleanUp of lane 2 and 5 with the Machery-Nagel-PCR-CleanUp-Kit

Lane2: 4-2106-451: 7.4 ng/µl

Lane5: 4-2106-451: 8.5 ng/µl

Biosafety

Porines









4.Week

MFC

Mediators

Cytochromes

Biosafety

Porines









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