Chloramphenicol stock solution

-Solubilize 20 mg mL-1 Chloramphenicol in 100 % Ethanol

-Store at -20 °C

DNA loading buffer

- 50 % (v/v) glycerol

- 1 mM EDTA

- 0.1 % (w/v) bromphenol blue

- Solve in ddH2O

LB medium

- 10 g Trypton

- 5 g yeast extract

- 10 g NaCl

- 12 g Agar-Agar (for plates)

- Adjust pH to 7.4

M9 medium

For 1 L M9 mineral medium you need 867 mL sterile water (for plates add 16 g Agar-Agar as well). Then add (in the following order:

- 300 µL 1 M CaCl2

1 M CaCl2

1 M CaCl2-H20

14.70 g/100 mL

- 100 mL 5x M9 salt solution

10x M9 salt solution
Na2HPO4-2H2O	75.2 g/L
KH2PO4	30 g/L
NaCl	5 g/L
NH4Cl	5 g/L

- 20 % glucose

20 % Glucose

20 % (w/v)glucose

200 g/L

For 500 mL stock solution add 100 g glucose to 440 mL water. Autoclave for 15 at 121° C

- 1 M MgSO4

MgSO4

1 M MgSO4-7H20

24.65 g/100 mL

- 1 mL biotin (1 mg/mL)

Biotin (1 mg/mL)

biotin (1 mg/mL)

50 mg/50 mL

For 50 mL stock solution dissolve 50 mg biotin in 45 mL water. Add water to a final volume of 50 mL. Sterilize the solution over a 0.22-µm filter. Prepare 1 mL aliquots and store at -20°.

- 1 mL thiamin (1 mg/mL)

Thiamin (1 mg/mL)

thiamin-HCl (1 mg/mL)

50 mg/50 mL

For 50 mL stock solution dissolve 50 mg thiamin-HCl in 45 mL water. Add water to a final volume of 50 mL. Sterilize the solution over a 0.22-µm filter. Prepare 1 mL aliquots and store at -20°.

- 10 mL 100x trace elements solution

100x trace elements solution	EDTA	5 g/L	13.4 mM
	FeCl3-6H2O	0.83 g/L	3.1 mM
	ZnCl2	84 mg/L	0.62 mM
	CuCl2-2H20	13 mg/L	76 µM
	CoCl2-H2O	10 mg/L	42 µM
	H3BO3	10 mg/L	162 µM
	MnCl2-4H20	1.6 mg/L	8.1 µM

Dissolve 5g EDTA in 800 mL water and adjust the pH to 7.5 with NaOH. Then add the other components in the quantities mentioned below and add water to a final volume of 1L. Sterilize the solution over a 0.22µm filter

498 mg	FeCl3(anhydrous)
84 mg	ZnCl2
765 µL	0.1 M CuCl2-2H20	1.70 g/100 mL
210 µL	0.2 M CoCl2-H2O	4.76 g/100 mL
1.6 mL	0.1 M H3BO3	0.62 g/100 mL
8.1 µL	1M MnCl2-4H20	19.8 g/100 mL

SOC medium

Add the following components for 900 ml of distilled H2O:

- 20 g Trypton

- 5 g Bacto Yeast Extract

- 2 mL of 5 M NaCl

- 2.5 ml of 1 M KCl

- 10 ml of 1 M MgCl2

- 10 ml of 1 M MgSO4

- 20 ml of 1 M glucose

Adjust to 1L with distilled H2O. Sterilize by autoclaving.