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08/08/13
From 2013.igem.org
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(→Making IPTG and chloroamphenicol plates) |
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| - | ==Making | + | ==Making chloroamphenicol/IPTG plates== |
*400ml of agar | *400ml of agar | ||
**Boil for 3min x2 | **Boil for 3min x2 | ||
| Line 21: | Line 21: | ||
*Add 800ul of chloroamphenicol at 25um/ml | *Add 800ul of chloroamphenicol at 25um/ml | ||
*Add 400ul IPTG at 0.15um/ml | *Add 400ul IPTG at 0.15um/ml | ||
| + | *Makes 20 petri dishes | ||
| + | |||
| + | ==Streaked out samples of the limonene/pSB1C3 ligated plasmid== | ||
| + | *Streaked samples 5.1, 10.1, 10.2 and 10.3 onto the chloroamphenicol/IPTG plates | ||
| + | *IPTG was used to induce the lac operon, and therefore induce limonene synthesis | ||
| + | *Plates were labelled using special symbols to ensure double-blinded test | ||
Revision as of 16:14, 8 August 2013
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Making chloroamphenicol/IPTG plates
- 400ml of agar
- Boil for 3min x2
- Boil for 2min x2
- Cool to 40C
- Add 800ul of chloroamphenicol at 25um/ml
- Add 400ul IPTG at 0.15um/ml
- Makes 20 petri dishes
Streaked out samples of the limonene/pSB1C3 ligated plasmid
- Streaked samples 5.1, 10.1, 10.2 and 10.3 onto the chloroamphenicol/IPTG plates
- IPTG was used to induce the lac operon, and therefore induce limonene synthesis
- Plates were labelled using special symbols to ensure double-blinded test
