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12/07/13
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| + | ==Transformation of RFP control plasmid== | ||
| + | |||
| + | *Keep competent cells on ice | ||
| + | **Add 50ul of cells to 4 tubes | ||
| + | *Add 1ul of resuspended DNA to 2 tubes | ||
| + | *Add 1ul RFP to the other 2 tubes | ||
| + | *Incubate for 30mins | ||
| + | *Heat shock at 42 degrees for 60secs | ||
| + | *Incubate on ice for 5mins | ||
| + | *Incubate at 37degrees for 2hrs | ||
Revision as of 10:12, 14 August 2013
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Transformation of RFP control plasmid
- Keep competent cells on ice
- Add 50ul of cells to 4 tubes
- Add 1ul of resuspended DNA to 2 tubes
- Add 1ul RFP to the other 2 tubes
- Incubate for 30mins
- Heat shock at 42 degrees for 60secs
- Incubate on ice for 5mins
- Incubate at 37degrees for 2hrs
