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14/08/13
From 2013.igem.org
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*Add 9ul of TE buffer to 6 epindorph tubes | *Add 9ul of TE buffer to 6 epindorph tubes | ||
| - | *Pipette 1ul of each sonicated DNA sample into each buffer | + | *Pipette 1ul of each sonicated DNA sample 1-6 into each buffer for a 10x dilution |
| + | *vortex thoroughly and centrifuge briefly | ||
| + | |||
| + | ==Running dilutions on 0.8% gel== | ||
| + | *1ul of dilution was put in 14ul of water and 5ul of orange G, to make up a total volume of 20ul for each sample. | ||
| + | *5ul of 1kb ladder was used on both ends as a marker | ||
| + | [[File:Igem_SON_dna_140813.jpg]] | ||
| + | |||
| + | |||
| + | ==Testing for limonene== | ||
| + | |||
| + | Took sample plates to chemistry lab | ||
Latest revision as of 10:21, 15 August 2013
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Dilutions of Herring Sperm DNA
- Add 9ul of TE buffer to 6 epindorph tubes
- Pipette 1ul of each sonicated DNA sample 1-6 into each buffer for a 10x dilution
- vortex thoroughly and centrifuge briefly
Running dilutions on 0.8% gel
- 1ul of dilution was put in 14ul of water and 5ul of orange G, to make up a total volume of 20ul for each sample.
- 5ul of 1kb ladder was used on both ends as a marker
Testing for limonene
Took sample plates to chemistry lab
