14/08/13

From 2013.igem.org

(Difference between revisions)
(Dilutions of Herring Sperm DNA)
 
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*5ul of 1kb ladder was used on both ends as a marker
*5ul of 1kb ladder was used on both ends as a marker
[[File:Igem_SON_dna_140813.jpg]]
[[File:Igem_SON_dna_140813.jpg]]
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==Testing for limonene==
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Took sample plates to chemistry lab

Latest revision as of 10:21, 15 August 2013

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Dilutions of Herring Sperm DNA

  • Add 9ul of TE buffer to 6 epindorph tubes
  • Pipette 1ul of each sonicated DNA sample 1-6 into each buffer for a 10x dilution
  • vortex thoroughly and centrifuge briefly

Running dilutions on 0.8% gel

  • 1ul of dilution was put in 14ul of water and 5ul of orange G, to make up a total volume of 20ul for each sample.
  • 5ul of 1kb ladder was used on both ends as a marker

Igem SON dna 140813.jpg


Testing for limonene

Took sample plates to chemistry lab