"
Exeter/31 July 2013
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Revision as of 11:35, 6 August 2013 by FelicityHeath (Talk | contribs)
Liquid cultures from the 30/07/2013
| Liquid culture | Did it work? |
|---|---|
| K592018 | yes |
| B0015 | yes |
| K592022 | no |
| K864404 | no |
| K592011 | no |
| S05058 | yes |
We then mini-prepped the liquid cultures that worked.
Mini Prep
We centrifuged the entire falcon tube for 10 minutes at 5000 rpm.
Then resuspended the whole pellet.
Nuclease free water was then run through instead of elution buffer, this was done twice.
Digest
Half was run on a gel, half was kept aside for ligation.
1 - RBS + Cph8 (cut with E + S)
2 - B0015 (cut with X + P)
3 - negative control (water)
4 -
