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• Team:Paris Saclay/ethanol
  = '''Ethanol precipitation''' = 2. Add 3x volumes of ethanol 100%
  741 B (113 words) - 14:23, 4 October 2013
• Team:NYMU-Taipei/Modeling/Ethanol
  =Ethanol model= ...ter are enzyme PDC and ADH-producing genes, which will convert pyruvate to ethanol, and thus kill spores of ''Nosema Ceranae''.
  10 KB (1,535 words) - 02:39, 29 October 2013
• Team:NYMU-Taipei/Modeling/Ethanol/explanation
  \frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.
  6 KB (928 words) - 14:09, 27 October 2013

Page text matches

• Team:Edinburgh/Project/Notebook
  ...f the culture). Those were inoculated with 1ml of differently concentrated ethanol solutions giving final concentrations of: ...ompetent ''E. coli'' cells were tested by transforming them with parts for ethanol production. The negative control was performed to confirm that cells have n
  41 KB (6,583 words) - 22:47, 4 October 2013
• Team:NYMU-Taipei/Project/Kill
  *Ethanol is known as a kind of toxins to bees. Up to certain degree, it has potentia *Ethanol can be produced from pyruvate through [http://parts.igem.org/Part:BBa_K110
  17 KB (2,669 words) - 03:06, 29 October 2013
• Team:NYMU-Taipei/Modeling/Ethanol
  =Ethanol model= ...ter are enzyme PDC and ADH-producing genes, which will convert pyruvate to ethanol, and thus kill spores of ''Nosema Ceranae''.
  10 KB (1,535 words) - 02:39, 29 October 2013
• Team:Concordia/Notebook
  <h2 class="log-title">Ethanol purification of EnvZ, and Fusions DOM/CON</h2> ... followed by 70% ethanol and re-suspension in Ultra Pure Distilled water. Ethanol Purification products were then verified and quantified using a 1% agarose
  16 KB (2,286 words) - 02:48, 28 September 2013
• Team:Edinburgh/Project/Results/Bioethanol Results
  <h3>Ethanol production using fused pdc and adhB:</h3> Pdc and adhB catalyse conversion of pyruvate to ethanol via acetaldehyde intermediate.
  8 KB (1,244 words) - 01:06, 5 October 2013
• Team:USP-Brazil/Results:FreezeDry
  <h4>Ethanol Resistance after Lyophilization</h4> ...od for our intended application, where the yeast will be resuspended using ethanol solution
  9 KB (1,304 words) - 04:02, 28 September 2013
• Team:USP-Brazil/Project
  ...on, <i>P.pastoris</i> cultures are able to grow in media with up to 10% of ethanol [10], which makes it a perfect candidate for our chassis. </p> ... means methanol will not be degraded as fast as it would in the absence of ethanol. Therefore, the degradation of peroxisomes would enhance the activation of
  18 KB (2,818 words) - 03:33, 28 September 2013
• Team:USP-Brazil/Model:Deterministic
  a report protein RFP with different concentrations of ethanol and methanol. By making some hypothesis, we ...xpression of the promoter in the presence of other sources of carbon, like ethanol. Because of
  10 KB (1,556 words) - 04:17, 28 September 2013
• Team:KIT-Kyoto/Notebook/ATF2
  was purified by ethanol precipitation</span></p> was purified by ethanol precipitation.</span></p>
  110 KB (16,118 words) - 02:26, 28 September 2013
• Team:USP-Brazil/Results:Characterization
  <h4>Chassis characterization, behavior of <i>Pichia pastoris</i> growth on ethanol solutions</h4> ...ity to different ethanol concentrations, this help us to define a specific ethanol range for input testing.</p>
  5 KB (812 words) - 01:20, 28 September 2013
• Team:RHIT/Protocol.html
  • Add ethanol (96-100%) to Buffer PE before use. </br> 3. Add 2x current volume ice cold ethanol.
  259 KB (27,268 words) - 01:38, 28 September 2013
• Team:Toronto/Notebook
  assays with ethanol, temperature and salt stimuli on clear plates<br/><br/> Amount of ethanol that needs to be pipetted for each stimulus treatment was
  30 KB (4,922 words) - 03:11, 28 September 2013
• Team:NJU China/Protocol
  </br>75% Ethanol (in DEPC-treated water) ...nt. Wash the RNA pellet once with 75% ethanol, adding at least 1 ml of 75% ethanol per 1 ml of TRIZOL Reagent used for the initial homogenization. Mix the sam
  69 KB (9,899 words) - 08:26, 28 October 2013
• Team:NJU China/test
  </br>75% Ethanol (in DEPC-treated water) ...nt. Wash the RNA pellet once with 75% ethanol, adding at least 1 ml of 75% ethanol per 1 ml of TRIZOL Reagent used for the initial homogenization. Mix the sam
  72 KB (10,248 words) - 02:00, 28 September 2013
• Team:Edinburgh/Modeling/Engineering model
  ...e-settling, biofilm formation and filtration, reverse osmosis sections and ethanol conversion. Ideally, all these four sections will be able to accomplish tas ...t the surface of the water by a skimming device, which is connected to the ethanol conversion section for biofilm production. Most, not necessarily all, insol
  9 KB (1,405 words) - 03:23, 5 October 2013
• Team:USP-Brazil/LaBioTec
  ...ent ethanol concentration on media by performing a <i>Pichia pastoris</i> Ethanol Survival test protocol. </br> To evaluate the viability of resuspended lyophilized samples in ethanol solutions 5 lyophilized samples were resuspended:<br/>
  15 KB (2,594 words) - 01:19, 28 September 2013
• Team:USP-Brazil/Parts
  ...ed in the carbon source-dependent regulation of Mxr1, which is inactive in ethanol and glycerol, but is active in methanol.</p> <p>Our submitted Mxr1 is mutated in order to NOT be repressed by ethanol, as the original Mxr1 is. This is done by substituted the Ser215 of the pro
  4 KB (613 words) - 00:42, 28 September 2013
• Team:Toronto/elementss
  increased in environments with 1% ethanol or NaCl. [9] <br/> <p style = "font-size:18px;"><b><u>Ethanol</u></b><br/>
  16 KB (2,473 words) - 00:48, 28 September 2013
• Team:Edinburgh/Project/Appendix
  '''Ethanol production''' Figure 3. Amount of ethanol produced following 24h aerobic incubation.
  8 KB (1,169 words) - 21:49, 4 October 2013
• Team:Toronto/Project/Elementss
  increased in environments with 1% ethanol or NaCl. [9] <br/><br/> <p style = "font-size:18px;"><b><u>Ethanol</u></b><br/>
  20 KB (3,131 words) - 03:41, 28 September 2013
• Team:METU Turkey/notebook.html
  <p>Hood is cleaned by ethanol, sterilized by uv light and shutted down.</p> ...en burner gases have been shutted down and checked, the bench cleaned with ethanol</p>
  68 KB (11,183 words) - 03:36, 5 October 2013
• Team:Kyoto/Material and method
  *Add 800&micro;L 80% ethanol and vortex.<br> ==Ethanol Precipitation==
  15 KB (2,547 words) - 12:47, 10 October 2013
• Team:Tianjin/Protocol
  .... Wash the column by adding 600μl of PW Wash Buffer diluted with absolute ethanol. Centrifuge at 12,000 rpm for 1 min at room temp.</p> <p>Note: PW Wash Buffer Concentrate must be diluted with absolute ethanol before use. See label for directions. If refrigerated, PW Wash Buffer must
  24 KB (3,678 words) - 21:20, 27 September 2013
• Team:BIOINT Mexico/Lab work
  *The supernatant was discarded and 500 µl of Wash Buffer with ethanol (w10) were added to the column *700 µl of Wash Buffer W9 with ethanol were added to the column
  41 KB (6,916 words) - 04:05, 28 September 2013
• Jamboree/Team Abstracts
  ...uld not only react to methanol, but also survive in high concentrations of ethanol. ... them with ease. Now all what it takes to finish the job is adding some ethanol to obtain our biodiesel!
  182 KB (27,381 words) - 14:33, 28 October 2013
• Team:Edinburgh/Introduction/Bioethanol
  ...iety of renewable, biomass-derived carbon sources compared to the standard ethanol producer: ''Sacharomyces cerevisiae''. ...ons catalysed by those enzymes (Figure 1) enable conversion of pyruvate to ethanol.
  3 KB (455 words) - 01:30, 5 October 2013
• Team:NYMU-Taipei/Modeling/Overview
  ...ates a sick bee timely when killing protein fails to kill Nosema, and that ethanol won’t kill a bee if it is cured in time. ...protein production and ethanol production more keenly. Killing protein and ethanol production effects an infected bee alternatively when the bee is in differe
  4 KB (564 words) - 03:07, 29 October 2013
• Read more20
  \frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.
  2 KB (226 words) - 02:22, 28 October 2013
• Team:ITU MOBGAM Turkey/protocol
  <p><strong>Ethanol precipitation of small fragments </strong></p><br> ...the sample is in a 1.5 mL eppendorf tube. I recommend storing the absolute ethanol at -20°C. </p>
  15 KB (2,323 words) - 22:49, 4 October 2013
• Team:NYMU-Taipei/Modeling/Ethanol/explanation
  \frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.
  6 KB (928 words) - 14:09, 27 October 2013
• More
  \frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.
  6 KB (928 words) - 14:16, 27 October 2013
• More7
  \frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.
  2 KB (226 words) - 00:46, 28 October 2013
• Team:KU Leuven/Protocols
  ...1ng/ml of MeS (Sigma-Aldrich, ReagentPlus®, ≥99% (GC)) in 97% pure ethanol or water. ...with the desired concentration for 10 minutes. MeSA was first suspended in ethanol before being further diluted to the desired concentration in 50ml water
  38 KB (6,008 words) - 03:58, 29 October 2013
• Jsher
  *Going to test Ethanol + NR with different amounts of ATC *Also going to repeat ATC and Ethanol experiment
  64 KB (10,685 words) - 04:42, 16 January 2014
• Team:NU Kazakhstan/Protocols
  <li>Ethanol (100% and 75%)</li> <li>The elutes (1 and 2) were diluted with 200 μl H2O (each) before ethanol precipitation;</li>
  14 KB (2,323 words) - 16:47, 25 September 2013
• Team:USP-Brazil/Protocols
  <li>Add 500 μL of ethanol 70%. Invert 5 times.</li> <h4>Ethanol Precipitation of Plasmid DNA</h4>
  19 KB (3,066 words) - 02:55, 27 September 2013
• Team:UC-Santa Cruz/Notebook3
  Ethanol ...e’s 10% ethanol in lab if not we can make 10% ethanol by adding 100ml of ethanol to 900ml
  27 KB (4,367 words) - 04:04, 28 September 2013
• (click here for detailed information about circuit design)
  We want the suicide part (ethanol) to be opened only after the first way (kill protein) fails; that is, Nosem ... time to go through the terminator barrier and thus, create a time lag for ethanol production.
  1 KB (192 words) - 02:42, 28 October 2013
• J Sher
  *Going to test Ethanol + NR with different amounts of ATC *Also going to repeat ATC and Ethanol experiment
  64 KB (10,685 words) - 04:40, 16 January 2014
• Team:Newcastle/HP/Safety
  ===Ethanol=== ...e lab to wipe down surfaces and pipettes. The main problem associated with ethanol is irritation especially in the eyes. It may cause pain or permanent cornea
  13 KB (2,157 words) - 21:10, 4 October 2013
• Team:Evry/Protocols
  <p><b>Chloramphenicol:</b><br/> Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL).<br> Add 400 μL of 50% ethanol and mix with a vortex or a pipette.<br>
  44 KB (6,816 words) - 03:48, 5 October 2013
• Team:Manchester/LabBookText
  .../ml ampicillin agar plates mate. 0.75g of ampicillin added to 15 ml of 50% ethanol to create 50 mg/ml stock to be kept in -20 ºC freezer. 0.4ml of this stock <p>Want a 34 mg/ml stock in 100% ethanol: 0.34 g in 10 ml of ethanol</p>
  26 KB (4,304 words) - 12:39, 30 September 2013
• Team:Manchester/LabBooktest
  .../ml ampicillin agar plates mate. 0.75g of ampicillin added to 15 ml of 50% ethanol to create 50 mg/ml stock to be kept in -20 ºC freezer. 0.4ml of this stock <p>Want a 34 mg/ml stock in 100% ethanol: 0.34 g in 10 ml of ethanol</p>
  60 KB (8,597 words) - 13:33, 26 October 2013
• Team:Evry/Protocols/
  <p><b>Chloramphenicol:</b><br/> Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL).<br> Add 400 μL of 50% ethanol and mix with a vortex or a pipette.<br>
  44 KB (6,791 words) - 02:37, 5 October 2013
• Team:Evry/Protocols/Test
  <p><b>Chloramphenicol:</b><br/> Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL).<br> Add 400 μL of 50% ethanol and mix with a vortex or a pipette.<br>
  44 KB (6,791 words) - 02:35, 5 October 2013
• Team:Manchester/test4
  .../ml ampicillin agar plates mate. 0.75g of ampicillin added to 15 ml of 50% ethanol to create 50 mg/ml stock to be kept in -20 ºC freezer. 0.4ml of this stock <p>Want a 34 mg/ml stock in 100% ethanol: 0.34 g in 10 ml of ethanol</p>
  59 KB (8,384 words) - 11:41, 2 October 2013
• Team:Manchester/LabBook
  .../ml ampicillin agar plates mate. 0.75g of ampicillin added to 15 ml of 50% ethanol to create 50 mg/ml stock to be kept in -20 ºC freezer. 0.4ml of this stock <p>Want a 34 mg/ml stock in 100% ethanol: 0.34 g in 10 ml of ethanol</p>
  62 KB (8,850 words) - 13:33, 26 October 2013
• Team:Newcastle/Notebook/protocols
  #Add 200 µl ethanol to the tube and vortex briefly. *Add ethanol (96-100%) to Buffer PE before use (see bottle label for volume).
  20 KB (3,259 words) - 20:49, 4 October 2013
• Team:Stanford-Brown/Projects/BioWires
  ... and 22µl of 3M CH3COONa was added to each tube (10% of final volume) for ethanol precipitation. Then 467 µl of isopropanol was added to each tube (70% of f ...econds each). It was then destained by dipping into another droplet of 50% ethanol for 5 seconds. The sample was dried again by wicking. Negative staining sam
  33 KB (5,289 words) - 05:36, 13 November 2013
• Team:Paris Bettencourt/Protocols
  <li>Add 500 ul of the Wash Solution (diluted with ethanol) to the GeneJET spin column. Centrifuge for 30-60 seconds and discard the f ...to remove residual Wash Solution. This step is essential to avoid residual ethanol in plasmid preps.
  29 KB (4,517 words) - 15:19, 16 October 2013
• Team:Utah State/Notebook
  ... with double distilled water (ddH2O) twice and then fill the cuvettes with ethanol.</li> <li>Let cuvettes sit with ethanol for 5-10 minutes, then wash 4-8 times with ddH2O</li>
  33 KB (4,425 words) - 03:24, 27 September 2013
• Team:SydneyUni Australia/Safety
  ...ed. Bacterial cells were always disposed of in labelled bottles containing ethanol. All equipment used for growth or transfer of bacterial cells were decontam |Ethanol
  20 KB (3,021 words) - 01:10, 29 October 2013
• Team:Gdansk-UG
  ... <p>We aim to construct bacteria able to detect methanol in ethanol solutions. Our project focuses on using a methanol-dependent promoter and ...sh; has methanol-dependent promoters which are blocked in the presence of ethanol. Therefore, we have found a bacterium which is an obligate methylotroph &n
  10 KB (1,160 words) - 16:49, 3 October 2013
• Team:TU-Munich/Results/Recombinant
  ...ve purification was carried out with buffers containing 5 mM of 2-mercapto-ethanol in all buffers. When comparing the size exclusion chromatograms, obtained f | Erythromycin in ethanol, 6 mM
  44 KB (6,706 words) - 03:19, 29 October 2013
• Team:Osaka/Human practice oc
  All you need is a cup of juice and ethanol. It’s pretty easy. ・20ml of 100%ethanol(cooled)
  3 KB (495 words) - 18:16, 27 September 2013
• Team:Osaka/Human practice
  All you need is a cup of juice and ethanol. It’s pretty easy. ・20ml of 100%ethanol(cooled)
  6 KB (927 words) - 03:56, 28 September 2013
• Team:Nanjing-China/protocol
  (5) Add a volume of 70% ethanol (check if anhydrous ethanol is added) to a volume of the solution from last step and mix thoroughly by (9) Add 700μL Buffer RW (check if anhydrous ethanol is added). Centrifuge at 12000rpm for 60s and discard the flow-through.<br/
  25 KB (3,655 words) - 15:21, 27 September 2013
• Team:TU-Munich/Notebook/Methods
  | Ethanol p.p., 99,9% Firstly, glass plates were wiped with 70% ethanol and then assembled onto a setting rig. No rubber spacers were added as they
  46 KB (6,472 words) - 01:29, 29 October 2013
• Team:TU-Eindhoven/LabJournal
  ...which had been stored in 100% Ethanol was lit in the flame burning off the Ethanol and sterilizing the spreader. ...ples we made a solution consisting of Coomassie Brilliant Blue G-250, 95% Ethanol and 85% Phosphoric Acid.
  244 KB (39,000 words) - 09:06, 26 October 2013
• Team:Edinburgh/Modeling/Waste treatment model
  ...much more data available for it, we decided to concentrate on modeling the ethanol production circuit. ... in the cell. We used the p’ number to determine the expressivity of the ethanol producing gene.
  2 KB (272 words) - 02:14, 5 October 2013
• Team:Evry/Notebook
  ...concentration of 25-170 µg/ml and at a storage concentration of 34 mg/ml (ethanol)<br/> ...ffective concentration of 10-50 and at a storage concentration of 5 mg/ml (ethanol)<br/></p>
  117 KB (16,033 words) - 02:34, 29 October 2013
• Team:HokkaidoU Japan/Safety
  <dt>Ethanol</dt><dd>flammable : must not be used near open flame or in large quantities ...Benson burner</dt><dd>fire risk: DO NOT use flammable materials especially ethanol near open fire</dd>
  10 KB (1,514 words) - 02:53, 29 October 2013
• Team:DTU-Denmark/Methods/Plasmid isolation
  {{:Team:DTU-Denmark/Templates/StartPage|Plasmid Isolation by ethanol precipitation}} # To the sample add 3 volumes of ice-cold 70% ethanol. If needed divide into several samples.
  902 B (140 words) - 17:40, 15 August 2013
• Team:Evry/Notebook/Test
  ...concentration of 25-170 µg/ml and at a storage concentration of 34 mg/ml (ethanol)<br/> ...ffective concentration of 10-50 and at a storage concentration of 5 mg/ml (ethanol)<br/></p>
  113 KB (15,206 words) - 01:49, 5 October 2013
• Team:Paris Saclay/Notebook/August/22
  ===='''3 - Ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI '''==== Protocol : [[Team:Paris_Saclay/ethanol|EtOH precipitation]]
  3 KB (475 words) - 01:16, 5 October 2013
• Team:KIT-Kyoto/Notebook/Protocol
  <p>Solution IV (5 M guanidine hydrochloride, 20 mM Tris-HCl, and 38% ethanol, pH 6.6 (25˚C))</p> <p>Solution V (2 mM Tris-HCl and 80% ethanol, pH 7.5 (25˚C))</p>
  10 KB (1,632 words) - 01:40, 28 September 2013
• Team:CAU China/Model
  ... be expressed in the strains and after lysis, the two enzymes can transfer ethanol into acetic acid in the stomach. Then drunkenness can be relieved. This mod ...ribe the enzyme catalysis reactions. With this model, we can know how much ethanol can be transferred successfully with a certain amount of enzymes.
  7 KB (1,061 words) - 16:46, 27 September 2013
• Team:UChicago/Protocols
  #Rinse the cuvette five times with 70% ethanol, again ensuring the chambers are fully washed. ...r syringe and hypodermic needle for small gaps) to remove as much residual ethanol as possible.
  20 KB (3,354 words) - 21:58, 25 October 2013
• Team:Tokyo-NoKoGen/Protocol
  14; Add 1 mL of 50% ethanol and resuspend. <br> 17; Dry pellet for a few minuet under a vacuum to remove residual ethanol.<br>
  25 KB (3,659 words) - 01:57, 28 September 2013
• Team:British Columbia/Notebook/Protocols/plates
  ...an - 50mg/mL in H2O, Chl - 35mg/mL in 100%? ethanol, Tet - 15 mg/ml in 50% ethanol - all stored at -20°C) *Ethanol everything (unopened plates package, Pipet gun, Pipets, Your arms up to the
  2 KB (399 words) - 00:50, 28 September 2013
• Team:USP-Brazil/Model:Stochastic
  ...transcription factor, methanol-binded transcription factor, free promoter, ethanol-binded promoter, methanol-binded promoter and RFP molecules inside the cell ...faster than any protein production. (ii)The intracellular concentration of ethanol and methanol does not change trouth the process due to <i>X_f</i>
  8 KB (1,284 words) - 10:51, 7 November 2013
• Team:HokkaidoU Japan/Notebook/Protocols
  <h2>Ethanol precipitation</h2> ...micro;L of NaOAc, 1.5 &micro;L of glycogen and (125) &micro;L of 100% ethanol.</li>
  13 KB (2,119 words) - 13:21, 28 October 2013
• Team:Hong Kong CUHK/protocol
  Note: Make sure that the amount of 100% ethanol specified on the bottle label has<br /> Note:    Make sure thatthe amount of 100% ethanol indicated on the bottle label has<br />
  28 KB (4,059 words) - 10:15, 27 October 2013
• Team:Edinburgh/Human Practices/Industries/Whisky
  ... process of heating the liquid so that it forms a vapour phase enriched in ethanol (the more volatile component) and a liquid phase enriched in water. The alc ...the initial effluent containing harmful methanol is separated from desired ethanol. This is performed manually using the lever combined with two separate coll
  7 KB (1,070 words) - 02:19, 5 October 2013
• Team:Edinburgh/Modeling/Modelling Results
  ... more resources than is estimated by the model. Additionally, the produces ethanol is toxic to the cell - and this is not captured by the model. ...erted circuit with those which have the antibiotic resistance but lack the ethanol producing gene.
  4 KB (696 words) - 02:58, 5 October 2013
• Team:KU Leuven/Journal/Ecology
  ...in a chemical safety cabinet to avoid distribution of the volatile MeS and ethanol.<br/> ... 0,01; 0,10; 0,40; 0,80 and 1,00 ng/ml. We made the dilutions in water and ethanol (EtOH). Since the plants have two different defence pathways depending on t
  9 KB (1,328 words) - 10:29, 3 January 2014
• Team:Gdansk-UG/Notebook
  ...> tolerance to methanol, tolerance of aerobic conditions and tolerance to ethanol. <br /> <td width="324" valign="top"><p>1% ethanol</p></td>
  28 KB (3,102 words) - 16:41, 4 October 2013
• Team:Heidelberg/Templates/Materials list
  ...stalline || Sigma-Aldrich Chemie GmbH || C0378-5G || 30 mg/ml || 1:3000 || Ethanol | Tetracycline || Sigma-Aldrich Chemie GmbH || T7660 || 10 mg/ml || 1:1000 || Ethanol
  95 KB (12,744 words) - 07:35, 15 January 2014
• Team:Braunschweig/Protocols
  3.4 g chloramphenicol was dissolved in 100 mL Ethanol, sterile filtered, aliquoted and stored at -20 °C.<br><br> 5 g Tetracyclin was dissolved in 100 mL Ethanol, sterile filtered aliqoted and stored at -20 °C.<br><br>
  22 KB (3,259 words) - 13:16, 27 October 2013
• Team:BYU Provo/Notebook/Cholera - Enzyme/May-June/Period1/Dailylog
  *95% ethanol ...er incubation times, absorbed stain is eluted from attached cells with 95% ethanol
  14 KB (2,082 words) - 23:41, 27 September 2013
• Team:UNITN-Trento/Project/Methyl Salicylate
  ...erience we performed other measurements to verify the equivalence of using ethanol and LB as a matrix for dissolving MeSA. Right panel: GC-FID in action </spa
  10 KB (1,579 words) - 22:55, 4 October 2013
• Exeter/3 July 2013
  Chloramphenicol must be dissolved in ethanol, the others can be dissolved in distilled water. ...dd 10 ml ethanol/water using a serological pippette, using a little of the ethanol/water to wash the last of the antibiotic from the Eppendorf. Seal each Falc
  2 KB (316 words) - 07:02, 30 September 2013
• Team:Grenoble-EMSE-LSU/Documentation/Notebook/August
  ...t during the Miniprep, it still may be some ethanol after the elution. And ethanol can led to the inhibition or the star-activity of the RE.</br> ...fore the elution, the column is warm up to 70°C for 5min in order for the ethanol to evaporate.</br>
  8 KB (1,093 words) - 01:28, 5 October 2013
• Team:Warsaw/Protocols
  ...rifuge at top speed for 10 minutes. Aspirate supernatant, add 200 &mul 70% ethanol and centrifuge at top speed for 1 minute. # Aspirate off all the ethanol supernatant. Dissolve the pellet in 40 &mu;l of water. Store at -20°C.
  10 KB (1,652 words) - 02:01, 5 October 2013
• Team:DTU-Denmark/Notebook/2 August 2013
  ===Purification of plasmids after ethanol purification===
  4 KB (534 words) - 20:45, 16 September 2013
• Team:DTU-Denmark/Notebook/30 August 2013
  ...rification of psB1C3 USER fragment to make Biobricks (escaped from well -> Ethanol?) * purification of HAO fragment (escaped from well -> Ethanol?)
  3 KB (385 words) - 12:09, 4 October 2013
• Team:Groningen/protocols/Genomic DNA extraction
  <li>70% ethanol RT</li> ...ain the tube on clean absorbent paper. Add 600 μl of room temperature 70% ethanol and gently invert the tube several times to wash the DNA pellet.</li>
  3 KB (452 words) - 15:36, 27 September 2013
• Team:Glendale CC AZ/Protocols/DNAIsolation
  -70% ethanol, room temperature 600μL of room temperature 70% ethanol and gently invert the tube several times to
  11 KB (1,346 words) - 02:36, 28 September 2013
• Team:Bielefeld-Germany/Labjournal/Molecular
  **10 minutes sonication of glass slides in ethanol. *Solubilize 20 mg mL^-1 Chloramphenicol in 100 % Ethanol
  48 KB (6,864 words) - 03:26, 29 October 2013
• Team:Paris Saclay/Notebook/July/1
  * Then, we inactivate EcoRI/SpeI activity by ethanol precipitation. Protocol : [[Team:Paris_Saclay/ethanol|Ethanol precipitation]]
  2 KB (277 words) - 00:48, 5 October 2013
• Team:KIT-Kyoto/nf08123
  Solution Ⅳ (5 M guanidine hydrochloride, 20 mM Tris-HCl, and 38% ethanol, pH 6.6 (25˚C)) Solution Ⅴ (2 mM Tris-HCl and 80% ethanol, pH 7.5 (25˚C))
  8 KB (1,222 words) - 04:24, 26 September 2013
• Team:INSA Toulouse/contenu/lab practice/notebook/protocols/backbone
  ...repared 80% ethanol to Binding Plate, let sit for 1min, apply vacuum until ethanol passes through, about 20s to 1 min. </li><li> Repeat ethanol wash (step 4) twice more for three washes total
  12 KB (1,848 words) - 09:55, 9 September 2013
• Team:Evry/Protocols/08
  Add 400 μL of 50% ethanol and mix with a vortex or a pipette.<br> Add 500 μL of Wash Buffer I (previously added with ethanol). Centrifuge at 1 600 x g. Discard the flow-through and place the purificat
  4 KB (620 words) - 09:57, 2 September 2013
• Team:Paris Saclay/Notebook/August/23
  ===='''1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI'''====
  1 KB (145 words) - 01:21, 5 October 2013
• Team:KU Leuven/Project/Ecological/wetlab
  ...ntrations and a control solution, which was the solvent used (in this case ethanol). The insects were given 1 to 3 mins to make a choice. After our first day ...t concentrations of MeS through the roots with water and via spraying with ethanol on the leaves. If you would like to find out how to practically induce a p
  27 KB (4,291 words) - 10:32, 3 January 2014
• Team:Georgia State/Notebook/august
  8. Add 250ul of chilled 100% ethanol 12. Wash the pellet with chilled 50ul of 70% ethanol then centrifuge at 10,000xg for 15minutes at 4°C<br />
  6 KB (954 words) - 22:50, 22 September 2013
• Team:Paris Saclay/ethanol
  = '''Ethanol precipitation''' = 2. Add 3x volumes of ethanol 100%
  741 B (113 words) - 14:23, 4 October 2013
• Team:British Columbia/Notebook/media
  ...an - 50mg/mL in H2O, Chl - 35mg/mL in 100%? ethanol, Tet - 15 mg/ml in 50% ethanol - all at -20°C)
  2 KB (275 words) - 00:59, 28 September 2013
• Team:USP-Brazil/Solution
  ...'s colorimetric method, currently, it is required by an ISO rule [3][4] in ethanol-producing industries. Most methanol detection devices rely on highly specif ...et al. Novel Method for Identification and Quantification of Methanol and Ethanol in Alcoholic Beverages by Gas Chromatography-Fourier Transform Infrared Spe
  5 KB (674 words) - 00:37, 28 September 2013
• Team:KIT-Kyoto/nf0812
  &#8547; (5 M guanidine hydrochloride, 20 mM Tris-HCl, and 38% ethanol, pH &#8548; (2 mM Tris-HCl and 80% ethanol, pH 7.5 (25&#730;C))</FONT></SPAN></FONT></P>
  27 KB (4,016 words) - 02:38, 26 September 2013