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  • Team:Paris Saclay/ethanol
    = '''Ethanol precipitation''' = 2. Add 3x volumes of ethanol 100%
    741 B (113 words) - 14:23, 4 October 2013
  • Team:NYMU-Taipei/Modeling/Ethanol
    =Ethanol model= ...ter are enzyme PDC and ADH-producing genes, which will convert pyruvate to ethanol, and thus kill spores of ''Nosema Ceranae''.
    10 KB (1,535 words) - 02:39, 29 October 2013
  • Team:NYMU-Taipei/Modeling/Ethanol/explanation
    \frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.
    6 KB (928 words) - 14:09, 27 October 2013

Page text matches

  • Team:Edinburgh/Project/Notebook
    ...f the culture). Those were inoculated with 1ml of differently concentrated ethanol solutions giving final concentrations of: ...ompetent ''E. coli'' cells were tested by transforming them with parts for ethanol production. The negative control was performed to confirm that cells have n
    41 KB (6,583 words) - 22:47, 4 October 2013
  • Team:NYMU-Taipei/Project/Kill
    *Ethanol is known as a kind of toxins to bees. Up to certain degree, it has potentia *Ethanol can be produced from pyruvate through [http://parts.igem.org/Part:BBa_K110
    17 KB (2,669 words) - 03:06, 29 October 2013
  • Team:NYMU-Taipei/Modeling/Ethanol
    =Ethanol model= ...ter are enzyme PDC and ADH-producing genes, which will convert pyruvate to ethanol, and thus kill spores of ''Nosema Ceranae''.
    10 KB (1,535 words) - 02:39, 29 October 2013
  • Team:Concordia/Notebook
    <h2 class="log-title">Ethanol purification of EnvZ, and Fusions DOM/CON</h2> ... followed by 70% ethanol and re-suspension in Ultra Pure Distilled water. Ethanol Purification products were then verified and quantified using a 1% agarose
    16 KB (2,286 words) - 02:48, 28 September 2013
  • Team:Edinburgh/Project/Results/Bioethanol Results
    <h3>Ethanol production using fused pdc and adhB:</h3> Pdc and adhB catalyse conversion of pyruvate to ethanol via acetaldehyde intermediate.
    8 KB (1,244 words) - 01:06, 5 October 2013
  • Team:USP-Brazil/Results:FreezeDry
    <h4>Ethanol Resistance after Lyophilization</h4> ...od for our intended application, where the yeast will be resuspended using ethanol solution
    9 KB (1,304 words) - 04:02, 28 September 2013
  • Team:USP-Brazil/Project
    ...on, <i>P.pastoris</i> cultures are able to grow in media with up to 10% of ethanol [10], which makes it a perfect candidate for our chassis. </p> ... means methanol will not be degraded as fast as it would in the absence of ethanol. Therefore, the degradation of peroxisomes would enhance the activation of
    18 KB (2,818 words) - 03:33, 28 September 2013
  • Team:USP-Brazil/Model:Deterministic
    a report protein RFP with different concentrations of ethanol and methanol. By making some hypothesis, we ...xpression of the promoter in the presence of other sources of carbon, like ethanol. Because of
    10 KB (1,556 words) - 04:17, 28 September 2013
  • Team:KIT-Kyoto/Notebook/ATF2
    was purified by ethanol precipitation</span></p> was purified by ethanol precipitation.</span></p>
    110 KB (16,118 words) - 02:26, 28 September 2013
  • Team:USP-Brazil/Results:Characterization
    <h4>Chassis characterization, behavior of <i>Pichia pastoris</i> growth on ethanol solutions</h4> ...ity to different ethanol concentrations, this help us to define a specific ethanol range for input testing.</p>
    5 KB (812 words) - 01:20, 28 September 2013
  • Team:RHIT/Protocol.html
    • Add ethanol (96-100%) to Buffer PE before use. </br> 3. Add 2x current volume ice cold ethanol.
    259 KB (27,268 words) - 01:38, 28 September 2013
  • Team:Toronto/Notebook
    assays with ethanol, temperature and salt stimuli on clear plates<br/><br/> Amount of ethanol that needs to be pipetted for each stimulus treatment was
    30 KB (4,922 words) - 03:11, 28 September 2013
  • Team:NJU China/Protocol
    </br>75% Ethanol (in DEPC-treated water) ...nt. Wash the RNA pellet once with 75% ethanol, adding at least 1 ml of 75% ethanol per 1 ml of TRIZOL Reagent used for the initial homogenization. Mix the sam
    69 KB (9,899 words) - 08:26, 28 October 2013
  • Team:NJU China/test
    </br>75% Ethanol (in DEPC-treated water) ...nt. Wash the RNA pellet once with 75% ethanol, adding at least 1 ml of 75% ethanol per 1 ml of TRIZOL Reagent used for the initial homogenization. Mix the sam
    72 KB (10,248 words) - 02:00, 28 September 2013
  • Team:Edinburgh/Modeling/Engineering model
    ...e-settling, biofilm formation and filtration, reverse osmosis sections and ethanol conversion. Ideally, all these four sections will be able to accomplish tas ...t the surface of the water by a skimming device, which is connected to the ethanol conversion section for biofilm production. Most, not necessarily all, insol
    9 KB (1,405 words) - 03:23, 5 October 2013
  • Team:USP-Brazil/LaBioTec
    ...ent ethanol concentration on media by performing a <i>Pichia pastoris</i> Ethanol Survival test protocol. </br> To evaluate the viability of resuspended lyophilized samples in ethanol solutions 5 lyophilized samples were resuspended:<br/>
    15 KB (2,594 words) - 01:19, 28 September 2013
  • Team:USP-Brazil/Parts
    ...ed in the carbon source-dependent regulation of Mxr1, which is inactive in ethanol and glycerol, but is active in methanol.</p> <p>Our submitted Mxr1 is mutated in order to NOT be repressed by ethanol, as the original Mxr1 is. This is done by substituted the Ser215 of the pro
    4 KB (613 words) - 00:42, 28 September 2013
  • Team:Toronto/elementss
    increased in environments with 1% ethanol or NaCl. [9] <br/> <p style = "font-size:18px;"><b><u>Ethanol</u></b><br/>
    16 KB (2,473 words) - 00:48, 28 September 2013
  • Team:Edinburgh/Project/Appendix
    '''Ethanol production''' Figure 3. Amount of ethanol produced following 24h aerobic incubation.
    8 KB (1,169 words) - 21:49, 4 October 2013
  • Team:Toronto/Project/Elementss
    increased in environments with 1% ethanol or NaCl. [9] <br/><br/> <p style = "font-size:18px;"><b><u>Ethanol</u></b><br/>
    20 KB (3,131 words) - 03:41, 28 September 2013
  • Team:METU Turkey/notebook.html
    <p>Hood is cleaned by ethanol, sterilized by uv light and shutted down.</p> ...en burner gases have been shutted down and checked, the bench cleaned with ethanol</p>
    68 KB (11,183 words) - 03:36, 5 October 2013
  • Team:Kyoto/Material and method
    *Add 800&micro;L 80% ethanol and vortex.<br> ==Ethanol Precipitation==
    15 KB (2,547 words) - 12:47, 10 October 2013
  • Team:Tianjin/Protocol
    .... Wash the column by adding 600μl of PW Wash Buffer diluted with absolute ethanol. Centrifuge at 12,000 rpm for 1 min at room temp.</p> <p>Note: PW Wash Buffer Concentrate must be diluted with absolute ethanol before use. See label for directions. If refrigerated, PW Wash Buffer must
    24 KB (3,678 words) - 21:20, 27 September 2013
  • Team:BIOINT Mexico/Lab work
    *The supernatant was discarded and 500 µl of Wash Buffer with ethanol (w10) were added to the column *700 µl of Wash Buffer W9 with ethanol were added to the column
    41 KB (6,916 words) - 04:05, 28 September 2013
  • Jamboree/Team Abstracts
    ...uld not only react to methanol, but also survive in high concentrations of ethanol. ... them with ease. Now all what it takes to finish the job is adding some ethanol to obtain our biodiesel!
    182 KB (27,381 words) - 14:33, 28 October 2013
  • Team:Edinburgh/Introduction/Bioethanol
    ...iety of renewable, biomass-derived carbon sources compared to the standard ethanol producer: ''Sacharomyces cerevisiae''. ...ons catalysed by those enzymes (Figure 1) enable conversion of pyruvate to ethanol.
    3 KB (455 words) - 01:30, 5 October 2013
  • Team:NYMU-Taipei/Modeling/Overview
    ...ates a sick bee timely when killing protein fails to kill Nosema, and that ethanol won’t kill a bee if it is cured in time. ...protein production and ethanol production more keenly. Killing protein and ethanol production effects an infected bee alternatively when the bee is in differe
    4 KB (564 words) - 03:07, 29 October 2013
  • Read more20
    \frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.
    2 KB (226 words) - 02:22, 28 October 2013
  • Team:ITU MOBGAM Turkey/protocol
    <p><strong>Ethanol precipitation of small fragments </strong></p><br> ...the sample is in a 1.5 mL eppendorf tube. I recommend storing the absolute ethanol at -20°C. </p>
    15 KB (2,323 words) - 22:49, 4 October 2013
  • Team:NYMU-Taipei/Modeling/Ethanol/explanation
    \frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.
    6 KB (928 words) - 14:09, 27 October 2013
  • More
    \frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.
    6 KB (928 words) - 14:16, 27 October 2013
  • More7
    \frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.
    2 KB (226 words) - 00:46, 28 October 2013
  • Team:KU Leuven/Protocols
    ...1ng/ml of MeS (Sigma-Aldrich, ReagentPlus®, ≥99% (GC)) in 97% pure ethanol or water. ...with the desired concentration for 10 minutes. MeSA was first suspended in ethanol before being further diluted to the desired concentration in 50ml water
    38 KB (6,008 words) - 03:58, 29 October 2013
  • Jsher
    *Going to test Ethanol + NR with different amounts of ATC *Also going to repeat ATC and Ethanol experiment
    64 KB (10,685 words) - 04:42, 16 January 2014
  • Team:NU Kazakhstan/Protocols
    <li>Ethanol (100% and 75%)</li> <li>The elutes (1 and 2) were diluted with 200 μl H2O (each) before ethanol precipitation;</li>
    14 KB (2,323 words) - 16:47, 25 September 2013
  • Team:USP-Brazil/Protocols
    <li>Add 500 μL of ethanol 70%. Invert 5 times.</li> <h4>Ethanol Precipitation of Plasmid DNA</h4>
    19 KB (3,066 words) - 02:55, 27 September 2013
  • Team:UC-Santa Cruz/Notebook3
    Ethanol ...e’s 10% ethanol in lab if not we can make 10% ethanol by adding 100ml of ethanol to 900ml
    27 KB (4,367 words) - 04:04, 28 September 2013
  • (click here for detailed information about circuit design)
    We want the suicide part (ethanol) to be opened only after the first way (kill protein) fails; that is, Nosem ... time to go through the terminator barrier and thus, create a time lag for ethanol production.
    1 KB (192 words) - 02:42, 28 October 2013
  • J Sher
    *Going to test Ethanol + NR with different amounts of ATC *Also going to repeat ATC and Ethanol experiment
    64 KB (10,685 words) - 04:40, 16 January 2014
  • Team:Newcastle/HP/Safety
    ===Ethanol=== ...e lab to wipe down surfaces and pipettes. The main problem associated with ethanol is irritation especially in the eyes. It may cause pain or permanent cornea
    13 KB (2,157 words) - 21:10, 4 October 2013
  • Team:Evry/Protocols
    <p><b>Chloramphenicol:</b><br/> Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL).<br> Add 400 μL of 50% ethanol and mix with a vortex or a pipette.<br>
    44 KB (6,816 words) - 03:48, 5 October 2013
  • Team:Manchester/LabBookText
    .../ml ampicillin agar plates mate. 0.75g of ampicillin added to 15 ml of 50% ethanol to create 50 mg/ml stock to be kept in -20 ºC freezer. 0.4ml of this stock <p>Want a 34 mg/ml stock in 100% ethanol: 0.34 g in 10 ml of ethanol</p>
    26 KB (4,304 words) - 12:39, 30 September 2013
  • Team:Manchester/LabBooktest
    .../ml ampicillin agar plates mate. 0.75g of ampicillin added to 15 ml of 50% ethanol to create 50 mg/ml stock to be kept in -20 ºC freezer. 0.4ml of this stock <p>Want a 34 mg/ml stock in 100% ethanol: 0.34 g in 10 ml of ethanol</p>
    60 KB (8,597 words) - 13:33, 26 October 2013
  • Team:Evry/Protocols/
    <p><b>Chloramphenicol:</b><br/> Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL).<br> Add 400 μL of 50% ethanol and mix with a vortex or a pipette.<br>
    44 KB (6,791 words) - 02:37, 5 October 2013
  • Team:Evry/Protocols/Test
    <p><b>Chloramphenicol:</b><br/> Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL).<br> Add 400 μL of 50% ethanol and mix with a vortex or a pipette.<br>
    44 KB (6,791 words) - 02:35, 5 October 2013
  • Team:Manchester/test4
    .../ml ampicillin agar plates mate. 0.75g of ampicillin added to 15 ml of 50% ethanol to create 50 mg/ml stock to be kept in -20 ºC freezer. 0.4ml of this stock <p>Want a 34 mg/ml stock in 100% ethanol: 0.34 g in 10 ml of ethanol</p>
    59 KB (8,384 words) - 11:41, 2 October 2013
  • Team:Manchester/LabBook
    .../ml ampicillin agar plates mate. 0.75g of ampicillin added to 15 ml of 50% ethanol to create 50 mg/ml stock to be kept in -20 ºC freezer. 0.4ml of this stock <p>Want a 34 mg/ml stock in 100% ethanol: 0.34 g in 10 ml of ethanol</p>
    62 KB (8,850 words) - 13:33, 26 October 2013
  • Team:Newcastle/Notebook/protocols
    #Add 200 µl ethanol to the tube and vortex briefly. *Add ethanol (96-100%) to Buffer PE before use (see bottle label for volume).
    20 KB (3,259 words) - 20:49, 4 October 2013
  • Team:Stanford-Brown/Projects/BioWires
    ... and 22µl of 3M CH3COONa was added to each tube (10% of final volume) for ethanol precipitation. Then 467 µl of isopropanol was added to each tube (70% of f ...econds each). It was then destained by dipping into another droplet of 50% ethanol for 5 seconds. The sample was dried again by wicking. Negative staining sam
    33 KB (5,289 words) - 05:36, 13 November 2013
  • Team:Paris Bettencourt/Protocols
    <li>Add 500 ul of the Wash Solution (diluted with ethanol) to the GeneJET spin column. Centrifuge for 30-60 seconds and discard the f ...to remove residual Wash Solution. This step is essential to avoid residual ethanol in plasmid preps.
    29 KB (4,517 words) - 15:19, 16 October 2013
  • Team:Utah State/Notebook
    ... with double distilled water (ddH2O) twice and then fill the cuvettes with ethanol.</li> <li>Let cuvettes sit with ethanol for 5-10 minutes, then wash 4-8 times with ddH2O</li>
    33 KB (4,425 words) - 03:24, 27 September 2013
  • Team:SydneyUni Australia/Safety
    ...ed. Bacterial cells were always disposed of in labelled bottles containing ethanol. All equipment used for growth or transfer of bacterial cells were decontam |Ethanol
    20 KB (3,021 words) - 01:10, 29 October 2013
  • Team:Gdansk-UG
    ... <p>We aim to construct bacteria able to detect methanol in ethanol solutions. Our project focuses on using a methanol-dependent promoter and ...sh; has methanol-dependent promoters which are blocked in the presence of ethanol. Therefore, we have found a bacterium which is an obligate methylotroph &n
    10 KB (1,160 words) - 16:49, 3 October 2013
  • Team:TU-Munich/Results/Recombinant
    ...ve purification was carried out with buffers containing 5 mM of 2-mercapto-ethanol in all buffers. When comparing the size exclusion chromatograms, obtained f | Erythromycin in ethanol, 6 mM
    44 KB (6,706 words) - 03:19, 29 October 2013
  • Team:Osaka/Human practice oc
    All you need is a cup of juice and ethanol. It’s pretty easy. ・20ml of 100%ethanol(cooled)
    3 KB (495 words) - 18:16, 27 September 2013
  • Team:Osaka/Human practice
    All you need is a cup of juice and ethanol. It’s pretty easy. ・20ml of 100%ethanol(cooled)
    6 KB (927 words) - 03:56, 28 September 2013
  • Team:Nanjing-China/protocol
    (5) Add a volume of 70% ethanol (check if anhydrous ethanol is added) to a volume of the solution from last step and mix thoroughly by (9) Add 700μL Buffer RW (check if anhydrous ethanol is added). Centrifuge at 12000rpm for 60s and discard the flow-through.<br/
    25 KB (3,655 words) - 15:21, 27 September 2013
  • Team:TU-Munich/Notebook/Methods
    | Ethanol p.p., 99,9% Firstly, glass plates were wiped with 70% ethanol and then assembled onto a setting rig. No rubber spacers were added as they
    46 KB (6,472 words) - 01:29, 29 October 2013
  • Team:TU-Eindhoven/LabJournal
    ...which had been stored in 100% Ethanol was lit in the flame burning off the Ethanol and sterilizing the spreader. ...ples we made a solution consisting of Coomassie Brilliant Blue G-250, 95% Ethanol and 85% Phosphoric Acid.
    244 KB (39,000 words) - 09:06, 26 October 2013
  • Team:Edinburgh/Modeling/Waste treatment model
    ...much more data available for it, we decided to concentrate on modeling the ethanol production circuit. ... in the cell. We used the p’ number to determine the expressivity of the ethanol producing gene.
    2 KB (272 words) - 02:14, 5 October 2013
  • Team:Evry/Notebook
    ...concentration of 25-170 µg/ml and at a storage concentration of 34 mg/ml (ethanol)<br/> ...ffective concentration of 10-50 and at a storage concentration of 5 mg/ml (ethanol)<br/></p>
    117 KB (16,033 words) - 02:34, 29 October 2013
  • Team:HokkaidoU Japan/Safety
    <dt>Ethanol</dt><dd>flammable : must not be used near open flame or in large quantities ...Benson burner</dt><dd>fire risk: DO NOT use flammable materials especially ethanol near open fire</dd>
    10 KB (1,514 words) - 02:53, 29 October 2013
  • Team:DTU-Denmark/Methods/Plasmid isolation
    {{:Team:DTU-Denmark/Templates/StartPage|Plasmid Isolation by ethanol precipitation}} # To the sample add 3 volumes of ice-cold 70% ethanol. If needed divide into several samples.
    902 B (140 words) - 17:40, 15 August 2013
  • Team:Evry/Notebook/Test
    ...concentration of 25-170 µg/ml and at a storage concentration of 34 mg/ml (ethanol)<br/> ...ffective concentration of 10-50 and at a storage concentration of 5 mg/ml (ethanol)<br/></p>
    113 KB (15,206 words) - 01:49, 5 October 2013
  • Team:Paris Saclay/Notebook/August/22
    ===='''3 - Ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI '''==== Protocol : [[Team:Paris_Saclay/ethanol|EtOH precipitation]]
    3 KB (475 words) - 01:16, 5 October 2013
  • Team:KIT-Kyoto/Notebook/Protocol
    <p>Solution IV (5 M guanidine hydrochloride, 20 mM Tris-HCl, and 38% ethanol, pH 6.6 (25˚C))</p> <p>Solution V (2 mM Tris-HCl and 80% ethanol, pH 7.5 (25˚C))</p>
    10 KB (1,632 words) - 01:40, 28 September 2013
  • Team:CAU China/Model
    ... be expressed in the strains and after lysis, the two enzymes can transfer ethanol into acetic acid in the stomach. Then drunkenness can be relieved. This mod ...ribe the enzyme catalysis reactions. With this model, we can know how much ethanol can be transferred successfully with a certain amount of enzymes.
    7 KB (1,061 words) - 16:46, 27 September 2013
  • Team:UChicago/Protocols
    #Rinse the cuvette five times with 70% ethanol, again ensuring the chambers are fully washed. ...r syringe and hypodermic needle for small gaps) to remove as much residual ethanol as possible.
    20 KB (3,354 words) - 21:58, 25 October 2013
  • Team:Tokyo-NoKoGen/Protocol
    14; Add 1 mL of 50% ethanol and resuspend. <br> 17; Dry pellet for a few minuet under a vacuum to remove residual ethanol.<br>
    25 KB (3,659 words) - 01:57, 28 September 2013
  • Team:British Columbia/Notebook/Protocols/plates
    ...an - 50mg/mL in H2O, Chl - 35mg/mL in 100%? ethanol, Tet - 15 mg/ml in 50% ethanol - all stored at -20°C) *Ethanol everything (unopened plates package, Pipet gun, Pipets, Your arms up to the
    2 KB (399 words) - 00:50, 28 September 2013
  • Team:USP-Brazil/Model:Stochastic
    ...transcription factor, methanol-binded transcription factor, free promoter, ethanol-binded promoter, methanol-binded promoter and RFP molecules inside the cell ...faster than any protein production. (ii)The intracellular concentration of ethanol and methanol does not change trouth the process due to <i>X<sub>f</sub></i>
    8 KB (1,284 words) - 10:51, 7 November 2013
  • Team:HokkaidoU Japan/Notebook/Protocols
    <h2>Ethanol precipitation</h2> ...micro;L of NaOAc, 1.5 &micro;L of glycogen and (125) &micro;L of 100% ethanol.</li>
    13 KB (2,119 words) - 13:21, 28 October 2013
  • Team:Hong Kong CUHK/protocol
    Note: Make sure that the amount of 100% ethanol specified on the bottle label has<br /> Note:    Make sure thatthe amount of 100% ethanol indicated on the bottle label has<br />
    28 KB (4,059 words) - 10:15, 27 October 2013
  • Team:Edinburgh/Human Practices/Industries/Whisky
    ... process of heating the liquid so that it forms a vapour phase enriched in ethanol (the more volatile component) and a liquid phase enriched in water. The alc ...the initial effluent containing harmful methanol is separated from desired ethanol. This is performed manually using the lever combined with two separate coll
    7 KB (1,070 words) - 02:19, 5 October 2013
  • Team:Edinburgh/Modeling/Modelling Results
    ... more resources than is estimated by the model. Additionally, the produces ethanol is toxic to the cell - and this is not captured by the model. ...erted circuit with those which have the antibiotic resistance but lack the ethanol producing gene.
    4 KB (696 words) - 02:58, 5 October 2013
  • Team:KU Leuven/Journal/Ecology
    ...in a chemical safety cabinet to avoid distribution of the volatile MeS and ethanol.<br/> ... 0,01; 0,10; 0,40; 0,80 and 1,00 ng/ml. We made the dilutions in water and ethanol (EtOH). Since the plants have two different defence pathways depending on t
    9 KB (1,328 words) - 10:29, 3 January 2014
  • Team:Gdansk-UG/Notebook
    ...> tolerance to methanol, tolerance of aerobic conditions and tolerance to ethanol. <br /> <td width="324" valign="top"><p>1% ethanol</p></td>
    28 KB (3,102 words) - 16:41, 4 October 2013
  • Team:Heidelberg/Templates/Materials list
    ...stalline || Sigma-Aldrich Chemie GmbH || C0378-5G || 30 mg/ml || 1:3000 || Ethanol | Tetracycline || Sigma-Aldrich Chemie GmbH || T7660 || 10 mg/ml || 1:1000 || Ethanol
    95 KB (12,744 words) - 07:35, 15 January 2014
  • Team:Braunschweig/Protocols
    3.4 g chloramphenicol was dissolved in 100 mL Ethanol, sterile filtered, aliquoted and stored at -20 °C.<br><br> 5 g Tetracyclin was dissolved in 100 mL Ethanol, sterile filtered aliqoted and stored at -20 °C.<br><br>
    22 KB (3,259 words) - 13:16, 27 October 2013
  • Team:BYU Provo/Notebook/Cholera - Enzyme/May-June/Period1/Dailylog
    *95% ethanol ...er incubation times, absorbed stain is eluted from attached cells with 95% ethanol
    14 KB (2,082 words) - 23:41, 27 September 2013
  • Team:UNITN-Trento/Project/Methyl Salicylate
    ...erience we performed other measurements to verify the equivalence of using ethanol and LB as a matrix for dissolving MeSA. Right panel: GC-FID in action </spa
    10 KB (1,579 words) - 22:55, 4 October 2013
  • Exeter/3 July 2013
    Chloramphenicol must be dissolved in ethanol, the others can be dissolved in distilled water. ...dd 10 ml ethanol/water using a serological pippette, using a little of the ethanol/water to wash the last of the antibiotic from the Eppendorf. Seal each Falc
    2 KB (316 words) - 07:02, 30 September 2013
  • Team:Grenoble-EMSE-LSU/Documentation/Notebook/August
    ...t during the Miniprep, it still may be some ethanol after the elution. And ethanol can led to the inhibition or the star-activity of the RE.</br> ...fore the elution, the column is warm up to 70°C for 5min in order for the ethanol to evaporate.</br>
    8 KB (1,093 words) - 01:28, 5 October 2013
  • Team:Warsaw/Protocols
    ...rifuge at top speed for 10 minutes. Aspirate supernatant, add 200 &mul 70% ethanol and centrifuge at top speed for 1 minute. # Aspirate off all the ethanol supernatant. Dissolve the pellet in 40 &mu;l of water. Store at -20°C.
    10 KB (1,652 words) - 02:01, 5 October 2013
  • Team:DTU-Denmark/Notebook/2 August 2013
    ===Purification of plasmids after ethanol purification===
    4 KB (534 words) - 20:45, 16 September 2013
  • Team:DTU-Denmark/Notebook/30 August 2013
    ...rification of psB1C3 USER fragment to make Biobricks (escaped from well -> Ethanol?) * purification of HAO fragment (escaped from well -> Ethanol?)
    3 KB (385 words) - 12:09, 4 October 2013
  • Team:Groningen/protocols/Genomic DNA extraction
    <li>70% ethanol RT</li> ...ain the tube on clean absorbent paper. Add 600 μl of room temperature 70% ethanol and gently invert the tube several times to wash the DNA pellet.</li>
    3 KB (452 words) - 15:36, 27 September 2013
  • Team:Glendale CC AZ/Protocols/DNAIsolation
    -70% ethanol, room temperature 600μL of room temperature 70% ethanol and gently invert the tube several times to
    11 KB (1,346 words) - 02:36, 28 September 2013
  • Team:Bielefeld-Germany/Labjournal/Molecular
    **10 minutes sonication of glass slides in ethanol. *Solubilize 20 mg mL<sup>-1</sup> Chloramphenicol in 100 % Ethanol
    48 KB (6,864 words) - 03:26, 29 October 2013
  • Team:Paris Saclay/Notebook/July/1
    * Then, we inactivate EcoRI/SpeI activity by ethanol precipitation. Protocol : [[Team:Paris_Saclay/ethanol|Ethanol precipitation]]
    2 KB (277 words) - 00:48, 5 October 2013
  • Team:KIT-Kyoto/nf08123
    Solution Ⅳ (5 M guanidine hydrochloride, 20 mM Tris-HCl, and 38% ethanol, pH 6.6 (25˚C)) Solution Ⅴ (2 mM Tris-HCl and 80% ethanol, pH 7.5 (25˚C))
    8 KB (1,222 words) - 04:24, 26 September 2013
  • Team:INSA Toulouse/contenu/lab practice/notebook/protocols/backbone
    ...repared 80% ethanol to Binding Plate, let sit for 1min, apply vacuum until ethanol passes through, about 20s to 1 min. </li><li> Repeat ethanol wash (step 4) twice more for three washes total
    12 KB (1,848 words) - 09:55, 9 September 2013
  • Team:Evry/Protocols/08
    Add 400 μL of 50% ethanol and mix with a vortex or a pipette.<br> Add 500 μL of Wash Buffer I (previously added with ethanol). Centrifuge at 1 600 x g. Discard the flow-through and place the purificat
    4 KB (620 words) - 09:57, 2 September 2013
  • Team:Paris Saclay/Notebook/August/23
    ===='''1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI'''====
    1 KB (145 words) - 01:21, 5 October 2013
  • Team:KU Leuven/Project/Ecological/wetlab
    ...ntrations and a control solution, which was the solvent used (in this case ethanol). The insects were given 1 to 3 mins to make a choice. After our first day ...t concentrations of MeS through the roots with water and via spraying with ethanol on the leaves. If you would like to find out how to practically induce a p
    27 KB (4,291 words) - 10:32, 3 January 2014
  • Team:Georgia State/Notebook/august
    8. Add 250ul of chilled 100% ethanol 12. Wash the pellet with chilled 50ul of 70% ethanol then centrifuge at 10,000xg for 15minutes at 4°C<br />
    6 KB (954 words) - 22:50, 22 September 2013
  • Team:Paris Saclay/ethanol
    = '''Ethanol precipitation''' = 2. Add 3x volumes of ethanol 100%
    741 B (113 words) - 14:23, 4 October 2013
  • Team:British Columbia/Notebook/media
    ...an - 50mg/mL in H2O, Chl - 35mg/mL in 100%? ethanol, Tet - 15 mg/ml in 50% ethanol - all at -20°C)
    2 KB (275 words) - 00:59, 28 September 2013
  • Team:USP-Brazil/Solution
    ...'s colorimetric method, currently, it is required by an ISO rule [3][4] in ethanol-producing industries. Most methanol detection devices rely on highly specif ...et al. Novel Method for Identification and Quantification of Methanol and Ethanol in Alcoholic Beverages by Gas Chromatography-Fourier Transform Infrared Spe
    5 KB (674 words) - 00:37, 28 September 2013
  • Team:KIT-Kyoto/nf0812
    &#8547; (5 M guanidine hydrochloride, 20 mM Tris-HCl, and 38% ethanol, pH &#8548; (2 mM Tris-HCl and 80% ethanol, pH 7.5 (25&#730;C))</FONT></SPAN></FONT></P>
    27 KB (4,016 words) - 02:38, 26 September 2013
  • Team:UniSalento Lecce/Protocols
    <li>Wash DNA pellet with 2 ml or 5 ml of room-temperature 70% ethanol, and centrifuge at≥4000 rpm for 20 min. Carefully decant the supernatant ... removing it from the centrifuge a
nd the collection tube.Note: Residual ethanol from Buffer NT3 might inhibit enzymatic reactions. Total removal ofethanol
    40 KB (6,637 words) - 17:16, 4 October 2013
  • Team:UC Chile/Protocols
    • Add 25 mL absolute ethanol.<br> • Dissolve 1.7 g chloramphenicol in 50 mL absolute ethanol.<br>
    40 KB (6,223 words) - 01:30, 13 October 2013
  • Team:Toronto/Project/Assays
    removed, bound crystal violet is mobilised with an ethanol:acetone solution and the &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;•350 <tt>μ</tt>L 80:20 ethanol:acetone stock solution<br>
    17 KB (2,745 words) - 03:09, 28 September 2013
  • Team:British Columbia/Notebook/Protocols/media
    ...an - 50mg/mL in H2O, Chl - 35mg/mL in 100%? ethanol, Tet - 15 mg/ml in 50% ethanol - all at -20°C)
    2 KB (275 words) - 01:10, 28 September 2013
  • Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols
    ...0xg for 1-2 min, mark with pen on eppendorf where liquid line is, pull off ethanol, add 1 mL water and mix, gently pellet beads again, discard water, repeat, ...er incubation times, absorbed stain is eluted from attached cells with 95% ethanol
    10 KB (1,539 words) - 19:48, 14 October 2013
  • Team:HokkaidoU Japan/Notebook/RBS SD8
    <li class="step"> <div class="step-tile"> <h2 class="step-title">Ethanol Precipitation</h2> </div> <div class="step-detail"> <div class="step-detail <li class="step"> <div class="step-tile"> <h2 class="step-title">Ethanol Precipitation</h2> </div> <div class="step-detail"> <div class="step-detail
    16 KB (2,298 words) - 16:36, 27 September 2013
  • Team:NYMU-Taipei/Modeling/MainParts
    ... is to know ADH production rate and when it can reach the concentration of ethanol pathway equilibrium.''' ...p model. It shows that without ''Nosema'' infection (ROS concentration=0), ethanol production is low enough to be ignored.
    20 KB (3,021 words) - 02:40, 29 October 2013
  • Team:British Columbia/protocol/media
    ...an - 50mg/mL in H2O, Chl - 35mg/mL in 100%? ethanol, Tet - 15 mg/ml in 50% ethanol - all at -20°C)
    2 KB (275 words) - 01:05, 28 September 2013
  • Exeter/4 June 2013
    *Alcohols, mainly ethanol *Yeast (currently to makes ethanol) converts ~90% of its glucose to ethanol
    3 KB (542 words) - 21:01, 4 October 2013
  • Team:Edinburgh/Project/Conclusions
    ...ables it to be used by iGEM teams to come. Moreover, the great increase in ethanol yields achieved using Pdc and AdhB fusion protein was the most spectacular ...ocus on creating multiple protein fusions to achieve even higher yields of ethanol or other substances. Moreover, this project demonstrated the metabolic stre
    3 KB (521 words) - 00:52, 5 October 2013
  • Team:Gdansk-UG/Project
    ...re aiming to construct a bacterium which would be able detect methanol in ethanol solutions.<br /> ...ystem and even death. Therefore, a test that would enable people to check ethanol alcohol purity would prevent a whole lot of cases of people hospitalized b
    10 KB (1,218 words) - 16:28, 3 October 2013
  • Team:Heidelberg/Tyrocidine week14 ms
    *** 15 µl for isopropanol / ethanol DNA precipitation **** wash pellet in ethanol, centrifuge for 5 mins and discard supernatant, let dry
    13 KB (1,590 words) - 16:15, 4 October 2013
  • To learn about this mechanism, click here.
    [[Image:NYMU_ pops ethanol.png|center]] ...two steps. In the improved model, lags between sensing Nosema invasion and ethanol production can be expressed, enabling terminator’s effect to show(to lear
    3 KB (431 words) - 04:08, 28 October 2013
  • Team:Braunschweig/Safety
    ...le work some ethanol was used to clean working space, with the advice that ethanol is easily flammable and should just be used for small areas.<br>
    7 KB (1,146 words) - 13:20, 27 October 2013
  • Team:UANL Mty-Mexico/Results/Protocols
    <li>Transfer the supernatant to a fresh tube containing 1 mL of 100% ethanol.</li> <li>Add 200 µL of 70% ethanol and vortex gently for 10 seconds.</li>
    17 KB (2,606 words) - 22:52, 27 September 2013
  • Team:USP-Brazil
    ...governments and independent producers to make high-throughput screening of ethanol drinks tainted will methanol. The device will be used as an initial low co ...P<sub>AOX1</sub> promoter, which is activated by methanol and repressed by ethanol. Several parts of the device must be tuned for proper function in <i>Pichia
    2 KB (326 words) - 19:50, 26 September 2013
  • Team:IIT Madras/Notebook
    ...he HiPura Silica kit added to 9 volume of milliQ and 10 volume of absolute Ethanol.) ...he HiPura Silica kit added to 9 volume of milliQ and 10 volume of absolute Ethanol.) was added to the pellet.
    8 KB (1,086 words) - 07:13, 7 July 2013
  • Team:Newcastle/Project/plants
    #Rinse seeds for 2 minutes in 70% (v/v) ethanol. The washing of seeds in ethanol and Milton’s sterilizing fluid is carried out to kill any bacteria residi
    18 KB (2,720 words) - 18:43, 28 October 2013
  • Team:Evry/Notebook/w2
    ...concentration of 25-170 µg/ml and at a storage concentration of 34 mg/ml (ethanol)<br/> ...ffective concentration of 10-50 and at a storage concentration of 5 mg/ml (ethanol)<br/></p>
    3 KB (430 words) - 13:56, 25 August 2013
  • Team:UNITN-Trento/Protocols
    ...alicylic Acid 2mM by a solution 1 M obtained by dissolving 0,138 g in 1 mL ethanol 70%. Then the culture were put for other 2 hours at 37&deg;C in agitation w ...nt. Before and after each injection the syringe was washed with acetone or ethanol for 3 times to eliminate any residual.
    40 KB (5,683 words) - 16:30, 3 October 2013
  • Http://2013.igem.org/Http://2013.igem.org/Team:TU-Delft/LabWork/Protocols
    ... flow-through is discarded before this additional centrifugation. Residual ethanol from Buffer PE may inhibit subsequent enzymatic reactions. They are right a ...nd centrifuge for an additional 1 min at 13000 rpm to remove the remaining ethanol.<br>
    10 KB (1,540 words) - 14:50, 19 August 2013
  • Exeter/17 July 2013
    '''5''' - Add a volume of 70% ethanol that is equak to 2x the original sample volume and vortex for 10s. '''7''' - Remove supernatant and air dry to ensure all ethanol is removed.
    7 KB (1,275 words) - 21:06, 2 October 2013
  • Team:Evry/Notebook/w13
    50ml of pure ethanol (98%)</br> ... to the litterature, the Eudragit was not that 'extremely' soluble in pure ethanol and did not take 20mn to dissolve. As a consequence, I sonicated for 45 min
    25 KB (3,477 words) - 10:49, 1 October 2013
  • Team:Colombia Uniandes/Journal
    <li>Transfer the upper aqueous phase to a tube with 800 µL of chilled 100% ethanol and mix by inversion.</li> <li>Wash the pellet with 500 µL of ethanol (100%) by vortex. Repeat step 9.</li>
    23 KB (3,678 words) - 02:30, 28 September 2013
  • Team:WLC-Milwaukee/Team
    ...y respond that he enjoys differential media assays, the smell of fresh 70% ethanol solutions, and just about any protocol that he can do while listening to Ha ...ite protocol is the Restriction Digest Protocol, her favorite lab smell is ethanol and sharpies, and she enjoys listening to the thug life album. Outside of
    19 KB (3,132 words) - 03:46, 28 September 2013
  • Team:DTU-Denmark/Notebook/3 August 2013
    ... 3-1a, TAT 3-2, TAT 2-1 using [[Team:DTU-Denmark/Methods/Plasmid_isolation|ethanol precipitation]]. Actually should have used the Midiprep kit from QIAgen. After ethanol precipitation continued with Midiprep kit step from step 9.
    1 KB (207 words) - 20:46, 16 September 2013
  • Team:TU-Eindhoven/PublicSafety
    ... colon and generate infections. Moreover, it can easily be eliminated with ethanol. ...s are needed to ensure safety, our bacteria can be easily eliminated using ethanol and they are not yet used in humans and animals. However we are aware that
    6 KB (897 words) - 17:23, 26 October 2013
  • Team:Penn State/ButanolProject
    ...strially relevant compound that can serve as a more efficient biofuel than ethanol. The project took on another goal when it was realized that an intermediary more similar to gasoline than other small alcohols such as ethanol. The fact that it is similar in
    11 KB (1,531 words) - 18:45, 27 September 2013
  • Team:Rutgers/testwiki
    ...ble energy resources. Although much effort has gone into the production of ethanol, other biofuels, such as butanol, are superior.</p> ...a> has a higher energy content, is less volatile, and is safer to use than ethanol. To develop strains of bacteria that produce high levels of 1-butanol we ha
    6 KB (806 words) - 18:17, 3 August 2013
  • Team:Glendale CC AZ/Protocols/AlkalineLysis
    -400µl 70% ethanol ...rd supernatant, removing residual liquid with pipet tip. Add 200-400ul 70% ethanol, agitate pellet gently and centrifuge at 10,000 rpm for 10 min to precipita
    11 KB (1,290 words) - 02:35, 28 September 2013
  • Team:Paris Saclay/Labwork
    * Ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI [[File:Pswrong.p * Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI [[File:Pswrong.p
    33 KB (5,021 words) - 00:59, 5 October 2013
  • Team:Paris Saclay/Protocols
    [[Team:Paris_Saclay/ethanol|Ethanol Precipitation ]]
    1 KB (168 words) - 20:40, 3 October 2013
  • Team:Paris Saclay/extraction
    Add 210 µl ethanol 100% to the sample, stringy precipitates are visible. Centrifuge the column for 1 min at 13,200*g to remove the residual ethanol.
    2 KB (387 words) - 09:22, 4 October 2013
  • Team:DTU-Denmark/Methods/Calibrating Electrodes
    # Prepare 96% ethanol solution # Put both probes in ethanol solution for 1 minute
    2 KB (340 words) - 18:09, 15 August 2013
  • Team:UC Davis/Notebook/Week 7
    ...href="http://parts.igem.org/Part:pSB3K3">pSB3K3</a> plasmid. We also tried ethanol precipitating some DNA in order to prepare it for electroporation. ...598010</a> into the DH10B strain. It took a little trial and error and the ethanol precipitations we did yesterday were not necessary after all, but hopefully
    6 KB (1,035 words) - 22:33, 27 September 2013
  • Team:Paris Saclay/Notebook/July/9
    Protocol : [[Team:Paris_Saclay/ethanol|Ethanol precipitation]]
    2 KB (291 words) - 23:56, 4 October 2013
  • Team:UC Davis/Protocols
    <li>Stock solutions can be made at 35 mg/ml in ethanol and kept at -20º C</li> <li>Add 700 µL Wash Buffer (W9) with ethanol to the column. Centrifuge the column at 12,000 x g for 1 minute. Discard th
    26 KB (4,246 words) - 22:26, 19 October 2013
  • Team:Purdue/Notebook/Protocols
    <LI>Wipe counter with ethanol ...l – The frozen stock solutions of chloramphenicol are at 25mg/ml in 100% ethanol and are marked with purple. The final concentration for LB liquid culture i
    13 KB (2,139 words) - 22:46, 27 September 2013
  • Team:Purdue/Human Practices/Girl Scout Biotechnology Badge
    ...alt helps remove proteins attached to DNA. For the precipitation of DNA in ethanol we used the diffusion of salt into water as a counter example. The students
    6 KB (1,035 words) - 01:53, 28 September 2013
  • Team:British Columbia/Notebook/CRISPR
    '''Results:''' Gel slurries were filtered and ethanol precipitated and quantified. Products were cut with E+S, ligated into PSB1C '''Results:''' Gel slurries were filtered and ethanol precipitated and quantified. Products were cut with E+S, ligated into PSB1C
    11 KB (1,740 words) - 03:05, 29 October 2013
  • Team:NYMU-Taipei/Modeling
    ...ut the presence of Nosema, we add several terminals behind promoter. Here, ethanol model is used to simulate how many terminals do we need as a threshold.
    2 KB (374 words) - 05:06, 28 October 2013
  • Team:MIT/MaterialsAndMethods
    Note. This protocol is for Rh-PE that has been suspended in ethanol. <LI> Sterilize the surface in 95% ethanol & dry before coating.</LI>
    41 KB (6,854 words) - 03:20, 29 October 2013
  • Team:TU-Delft/Notebook/2013/08/28/
    </left><br>One set of Back bone was freeze squeezed and Ethanol purification was carried out. Other set was gel extracted and purification 7. The pBAD:receiver was run on gel, and gel extraction was done, followed by Ethanol purification. <br>
    2 KB (253 words) - 09:00, 4 October 2013
  • Team:INSA Toulouse/contenu/lab practice/notebook/protocols/comp cells
    · Place tubes into a zip lock bag, immerse bag into a dry ice/ethanol bath for 5 minutes<br> · Ethanol treat all working areas for sterility.<br>
    11 KB (1,624 words) - 09:18, 3 September 2013
  • Team:SDU-Denmark/Tour53
    ...ical evaluation of solubility of the polyisoprene. Cells were sonicated in ethanol suspension, washed in acetone, and extracted in n-hexane (both steps were o ...tionship. Some peaks from the solvents used to purify the rubber (Acetone, Ethanol, and Hexane), as well as a small amount of water (1.56 ppm) are seen, too.
    33 KB (5,182 words) - 02:44, 29 October 2013
  • Team:UCL/Project/Safety
    ...re used to minimise possible contamination when experiments are performed. Ethanol is applied on work surfaces before and after experimental procedures in ord <td>High concentration Ethanol (EtOH) and other alcohols</td>
    13 KB (2,054 words) - 02:51, 5 October 2013
  • Team:Wageningen UR/Experimental protocols
    Dry ice (liquid nitrogen), ethanol bath or liquid nitrogen <br /> <br /> 19. Shock freeze cell suspensions in a dry ice and ethanol bath. <br />
    35 KB (5,505 words) - 03:56, 5 October 2013
  • Team:DTU-Denmark/Methods/USER Cloning and Transformation
    96% Ethanol *Plate bacteria using a sterile Drigalsky (sterilize with ethanol and flame between each transformation - remember to cool down the spatchula
    3 KB (400 words) - 07:30, 30 September 2013
  • Team:Freiburg/Project/induction
    ... only shows very little differences in SEAP expression between hormone and ethanol treatment (in both directions) but 1.5- to 3-fold repression when Cas9-ERT2 ...ry slight difference; in most samples the repression is even stronger with ethanol addition than with tamoxifen (Figure 7).
    46 KB (7,044 words) - 03:50, 29 October 2013
  • Team:Paris Saclay/Notebook/August/26
    Protocol : [[Team:Paris_Saclay/ethanol|Ethanol precipitation]]
    4 KB (577 words) - 22:16, 4 October 2013
  • Team:Paris Saclay/Notebook/July/26
    Protocol : [[Team:Paris_Saclay/ethanol|Ethanol precipitation]]
    4 KB (632 words) - 00:29, 5 October 2013
  • Team:Paris Saclay/Notebook/July/31
    Protocol : [[Team:Paris_Saclay/ethanol|Ethanol precipitation]]
    3 KB (433 words) - 01:00, 5 October 2013
  • Team:Paris Saclay/Notebook/July/30
    Protocol : [[Team:Paris_Saclay/Protocols/Ethanol precipitation|Ethanol precipitation]]
    7 KB (1,029 words) - 00:58, 5 October 2013
  • Team:Berkeley/HumanPractice
    ..., Sasaki, C., & Nakamura, Y. (2010). Effective enzyme saccharification and ethanol production from Japanese cedar using various pretreatment methods. Journal ...n, Z., & Zhang, R. (2009). Overview of biomass pretreatment for cellulosic ethanol production. International Journal, 2, 51–68. doi:10.3965/j.issn.1934-6344
    8 KB (1,315 words) - 03:27, 28 September 2013
  • Team:Cornell/project/safety
    ...iotics, which could be harmful to humans in large doses. We also work with ethanol lamps to maintain a sterile environment, which do involve having an open fl ... and eye protection. The delineated and surrounding areas are cleaned with ethanol and/or bleach prior to and immediately following each use, and if these sub
    10 KB (1,560 words) - 00:57, 29 October 2013
  • Team:CAU China/Project2
    To mimic the normal metabolism of ethanol in human liver, we tried to present a recombinant strain occupying active A ...ure the extinction of our bacteria in digestiontrack correspond to the low ethanol concentration, a promoter which is able to sense the alcohol concentration
    3 KB (533 words) - 13:57, 27 September 2013
  • Team:Colombia Uniandes/ChimiJournal
    <li>Transfer the upper aqueous phase to a tube with 800 µL of chilled 100% ethanol and mix by inversion.</li> <li>Wash the pellet with 500 µL of ethanol (100%) by vortex. Repeat step 9.</li>
    9 KB (1,466 words) - 06:05, 27 September 2013
  • Team:Paris Saclay/Notebook/July/22
    Protocol : [[Team:Paris_Saclay/ethanol|Ethanol precipitation]]
    1 KB (222 words) - 15:47, 4 October 2013
  • Team:RHIT/Notebook.html
    ...I/SpeI in 1X CutSmart and BSA. Ran Gel of Digest, extracted bands and used ethanol/NaCl precipitation to concentrate all samples of each type into one sample. Concentrated DNA from Friday and Monday with Ethanol precipitation, ran gel for concentration, and Ligated fragments. Transform
    228 KB (23,275 words) - 08:54, 26 September 2013
  • Team:Colombia Uniandes/Protocols
    <li>200 µL of ethanol (100%) to the lysate – Vortex. --> Transfer the entire contents of the tu <li>500 µL of Wash Solution Concentrate (+ ethanol) --> 15000 rpm x 3 min – Discard the collection tube and place the column
    5 KB (900 words) - 22:43, 27 September 2013
  • Team:USP-Brazil/Safety
    ...ource in <i>P. pastoris</i>. It is activated by methanol, whereas glucose, ethanol and glycerol act as repressors. The P<sub>AOX</sub> promoter is found three ...ia pastoris. Our submitted part is mutated in order to not be repressed by ethanol, as the original Mx1r is. The submitted code is BBa_K1078000.</td><td><i>Pi
    19 KB (2,930 words) - 01:16, 28 September 2013
  • Team:BIOSINT Mexico/Protocols
    *Discard the supernatant and add 500 ml of Wash Buffer with ethanol (w10) to the column *Add 700ml of Wash Buffer W9 with ethanol to the column
    9 KB (1,528 words) - 04:02, 28 September 2013
  • Team:Freiburg/protocols
    ...pellet was washed with 70% ethanol and centrifuged for another 15 minutes. Ethanol was finally carefully removed and the DNA resuspended in an adequate volume
    29 KB (4,493 words) - 05:11, 28 October 2013
  • Team:CAU China/Protocal
    (9) Pipette 500μl of WB solution (containing ethanol) into the column, spin at 12000 rpm for 30 s and discard the liquid in the <td width="142" nowrap="nowrap" valign="bottom"><p>100% Ethanol</p></td>
    18 KB (2,587 words) - 06:07, 27 September 2013
  • Team:Goettingen/NoteBook w15
    ...; &nbsp; &nbsp; &nbsp; </span><span class="c0">clean bench and pipets with ethanol</span></p> ..."><span class="c0">c) binding of RNA: add more or less equal volume of 70% ethanol (RNA) (here: 1 ml) to flow-through, mix by pipetting and vortexing, then lo
    56 KB (9,352 words) - 20:20, 30 September 2013
  • Team:Goettingen/NoteBook w14
    ...><span class="c0">- &nbsp; &nbsp; &nbsp; &nbsp;clean bench and pipets with ethanol</span></p> ..."><span class="c0">c) binding of RNA: add more or less equal volume of 70% ethanol (RNA) (here: 1 ml) to flow-through, mix by pipetting and vortexing, then lo
    129 KB (20,625 words) - 21:12, 30 September 2013
  • Team:York UK/Project.html
    <li>Benchtops were decontaminated with ethanol before and after lab work was conducted;</li> <li>Tools that came into contact with bacteria were soaked in ethanol and flame-treated before and after use;</li>
    64 KB (9,800 words) - 00:54, 5 October 2013
  • Team:York UK/Outreach.html
    ... more visible and scooped out into a smaller PCR tube which also contained ethanol. We then added a drop of food colouring to make the DNA more visible and th
    11 KB (1,810 words) - 00:33, 5 October 2013
  • Team:HokkaidoU Japan/Notebook/RBS SD2,4,6
    <li class="step"> <div class="step-tile"> <h2 class="step-title">Ethanol precipitation</h2> </div> <div class="step-detail"> <div class="step-detail <li class="step"> <div class="step-tile"> <h2 class="step-title">Ethanol precipitation</h2> </div> <div class="step-detail"> <div class="step-detail
    4 KB (543 words) - 16:37, 27 September 2013
  • Team:IIT Delhi/Protocol
    10. Add 750uL PE Buffer (make sure 70% ethanol was added previously) and repeat step 9.<br> 7. Add 750uL PE Buffer (make sure 70% ethanol was added previously) and repeat step 6.<br>
    17 KB (2,496 words) - 04:17, 28 September 2013
  • Team:USP-Brazil/ExpertsOpinion
    ... is ingested with ethanol (as in the case of alcoholic beverages), because ethanol acts as an antidote for the formation of toxic metabolites of methanol. How
    6 KB (905 words) - 00:48, 28 September 2013
  • Team:IIT Madras/Protocol
    volume of Milli-Q and 10 volume of absolute ethanol.) Ethanol.) was added to the pellet.
    8 KB (1,198 words) - 23:48, 27 September 2013
  • Team:Rutgers/Safety
    <li>Ethanol<br> ... lab notebooks and protocols in the brown binder. Wipe the bench with some ethanol at the end of the day.</p>
    17 KB (2,283 words) - 03:40, 28 September 2013
  • Team:Tianjin/Project/Introduction
    ...7.0 MJ/kg compared with -29.7 MJ/kg for ethanol [2]. Second, compared with ethanol, the first generation biofuel, with a freezing point of -114℃, alkanes ha
    23 KB (2,976 words) - 00:26, 29 October 2013
  • Team:Heidelberg/Templates/DelH week17
    * 1 ml isopropanol was added and the normal isoprop-ethanol-preipitation * Weight 100 mg of tetracycline and dissolve it in 10 ml ethanol = stock solution
    25 KB (3,451 words) - 13:48, 25 October 2013
  • Team:Edinburgh/Introduction
    ...enia Team Slovenia 2010]), we wanted to exploit this principle to increase ethanol production in bacteria by generating[[Team:Edinburgh/Project/Results/Bioeth
    3 KB (471 words) - 01:03, 5 October 2013
  • Team:Warsaw/Glossary
    * '''ethanol''' – lots of ethanol in cell lab (take a deep breath ladies and gentelmen - unless one of you is
    3 KB (465 words) - 03:46, 5 October 2013
  • Team:Heidelberg/Templates/DelH week8
    * Add 750 µl 70% ethanol * Add 750 µl 70% ethanol
    7 KB (953 words) - 08:12, 24 October 2013
  • Team:Edinburgh/Project/Results/Chassis
    ...the growth curves at various ethanol concentrations to confirm the highest ethanol concentration suitable for cell growth, which was 4%, as displayed below. A
    4 KB (583 words) - 23:14, 4 October 2013
  • Team:Berkeley/HumanPractice/Introduction
    ..., Sasaki, C., & Nakamura, Y. (2010). Effective enzyme saccharification and ethanol production from Japanese cedar using various pretreatment methods. Journal ...n, Z., & Zhang, R. (2009). Overview of biomass pretreatment for cellulosic ethanol production. International Journal, 2, 51–68. doi:10.3965/j.issn.1934-6344
    9 KB (1,334 words) - 02:35, 24 October 2013
  • Team:Berkeley/HumanPractice/ABPDU
    ..., Sasaki, C., & Nakamura, Y. (2010). Effective enzyme saccharification and ethanol production from Japanese cedar using various pretreatment methods. Journal ...n, Z., & Zhang, R. (2009). Overview of biomass pretreatment for cellulosic ethanol production. International Journal, 2, 51–68. doi:10.3965/j.issn.1934-6344
    6 KB (864 words) - 03:27, 29 October 2013
  • Team:TU-Munich/Notebook/Labjournal
    *Wash pellet with 200&nbsp;µl 70&nbsp;% ethanol, centrifugation at 16,200&nbsp;g for 40&nbsp;min, supernatant discarded. Th
    804 KB (118,126 words) - 22:30, 22 October 2013
  • Team:Waterloo
    ...e in appropriate biological waste boxes. All surfaces were wiped down with ethanol after use and all glassware was washed immediately after their usage.
    182 KB (26,875 words) - 03:50, 29 October 2013
  • Team:TU-Munich/Notebook/Materials
    | Ethanol p.p., 99,9%
    13 KB (1,642 words) - 01:47, 5 October 2013
  • Team:Costa Rica Cibus/Project
    ...t term we expect to insert a set of genes that may transform glycerol into ethanol, a necesary compund that combined with triacyl produce biodiesel.
    4 KB (554 words) - 03:31, 28 September 2013
  • Team:Hong Kong HKUST/notebook/mod1
    <li>Made sodium palmitate from powder to solution by 50% Ethanol</li>
    24 KB (3,788 words) - 23:13, 27 September 2013
  • Team:BYU Provo/Safety
    ...hazard waste as well as cleaning glassware and desktops with bleach and/or ethanol.
    2 KB (342 words) - 00:29, 27 September 2013
  • Team:OU-Norman OK/Project/Notebook
    <li>Add 50&#956;L of 10% ethanol to get rid of isopropanol</li>
    189 KB (32,035 words) - 04:17, 28 September 2013
  • Team:Greensboro-Austin/Smell degradation
    ...ntrations of 2mM, 3mM and 4mM from a working stock of 0.5M ''p''-cresol in ethanol solution along with a control flask with media and the lowest concentration
    16 KB (2,288 words) - 01:23, 26 October 2013
  • Team:Georgia State/protocols
    <div class="accordionButton">Ethanol Precipitation of DNA</div>
    3 KB (423 words) - 03:15, 27 September 2013
  • Team:MSOE Milwaukee/Project
    ...oring in baked goods and beverages, preventing the separation problem with ethanol and petrol fuel blends, and it can even be used as a fuel. However, it is n
    5 KB (789 words) - 22:23, 22 September 2013
  • Tim Baker
    ...stock of Chloramphenicol from the solid and dissolved 20 mg/mL in 10 mL of ethanol
    14 KB (2,183 words) - 18:01, 27 September 2013
  • Team:Utah State/Team
    ...ng at Utah State. He has worked on the production of acetone, butanol, and ethanol from Clostridium as other research projects during his time at USU. Neal en
    10 KB (1,153 words) - 21:48, 27 September 2013
  • 17 June 2013
    <li>Precipitation (eluted DNA was diluted with water, and then 100% ethanol, ammonium acetate and glycogen were added). The mixture was incubated at -8
    1 KB (173 words) - 14:26, 17 June 2013
  • Team:Edinburgh/Project
    ...ised it thoroughly and demonstrated that such co-localisation can increase ethanol production at least twofold.'''
    1 KB (211 words) - 23:57, 4 October 2013
  • Team:UCL/Attributions
    ...njoy picnics, travelling and have an unfathomable passion for food and 70% ethanol.</p>
    8 KB (1,129 words) - 19:27, 3 October 2013
  • Team:Evry/Team
    ...i>, an anaerobic bacterium from forest soil that ferments plant biomass to ethanol and hydrogen. He did his PhD in microbial genomics at MIT and his post-doc
    14 KB (2,236 words) - 07:25, 11 December 2013
  • Exeter/8 July 2013
    The MiniPrep kit needs 35 ml ethanol added to the "Wash Solution" and RNAase 2 added to the "Resuspension Soluti
    3 KB (563 words) - 20:38, 1 October 2013
  • Team:Groningen/25 June 2013
    <br/>70% ethanol
    529 B (83 words) - 11:11, 28 June 2013
  • Team:Tokyo-NoKoGen/labosafety
    ... hands are clean (although we have gloves on), we spray our hands with 70% ethanol.
    15 KB (2,029 words) - 01:30, 28 September 2013
  • Team:DTU-Denmark/Methods
    [[Team:DTU-Denmark/Methods/Plasmid_isolation|Plasmid isolation by ethanol precipitation]]
    2 KB (246 words) - 12:31, 1 October 2013
  • Team:Queens Canada/Parts
    ...croorganisms must be enabled to produce certain types of alcohols, such as ethanol, isobutanol, and n-butanol and the last step in this process involves
    5 KB (683 words) - 23:47, 27 September 2013
  • Team:Yale/Project Overview
    ...ted to knock out any competing reactions, such as converting acetyl-CoA to ethanol, as well as preventing reactions that progressed in the opposite direction
    14 KB (1,903 words) - 00:27, 27 September 2013
  • Team:Groningen/Labwork/25 June 2013
    <br/>-70% ethanol
    1 KB (210 words) - 09:18, 30 July 2013
  • Team:WLC-Milwaukee
    ...amount of bioavailable glucose there would be an increased availability of ethanol for use as a renewable fuel source. In addition with an increase in bioavai
    6 KB (910 words) - 02:50, 28 September 2013
  • Team:Groningen/Labwork/12 July 2013
    <br/> Chloramphenicol 5mg/ml Ethanol 50%
    3 KB (425 words) - 10:14, 30 July 2013
  • Team:UGA-Georgia
    <li> It has a higher energy density than Ethanol, thereby having better fuel properties.
    3 KB (381 words) - 17:39, 27 September 2013
  • Team:WLC-Milwaukee/Project
    ...amount of bioavailable glucose there would be an increased availability of ethanol for use as a renewable fuel source. In addition with an increase in bioavai
    8 KB (1,200 words) - 01:35, 13 August 2013
  • Team:USP-Brazil/Problem
    ...d to consuming alcoholic drinks are attributed to intoxication by methanol/ethanol [2]. The main victims of those injuries are people from low and middle-inco
    6 KB (902 words) - 00:37, 28 September 2013
  • Team:Berkeley/Project
    ...en it is under stress. The leaf that is turning blue has been sprayed with ethanol, and parts of the plant may have begun to degrade. Though the plant is stil
    7 KB (893 words) - 18:30, 22 October 2013
  • Team:Penn State/Project
    ...strially relevant compound that can serve as a more efficient biofuel than ethanol. The project took on another goal when it was realized that an intermediary
    6 KB (859 words) - 15:36, 26 July 2013
  • Team:Bielefeld-Germany/Project/MFC
    ...tation under formation of metabolic end products like lactate, acetate and ethanol has to be avoided by the choice of suitable cultivation parameters. Their f
    60 KB (9,290 words) - 03:23, 29 October 2013
  • Team:Hong Kong HKUST/protocols
    ...s://static.igem.org/mediawiki/2013/2/25/19._Ethanol_Precipitation.pdf"><h3>Ethanol Precipitation</h3></a><br></div>
    10 KB (1,434 words) - 23:23, 27 September 2013
  • Team:DTU-Denmark/Notebook/30 July 2013
    ...O colony 2 and colony 3 using [[Team:DTU-Denmark/Methods/Plasmid_isolation|ethanol precipitation]].
    3 KB (519 words) - 20:44, 16 September 2013
  • Team:Paris Saclay/Notebook/August/19
    Protocol : [[Team:Paris_Saclay/ethanol|EtOH precipitation]]
    4 KB (520 words) - 01:29, 5 October 2013
  • Team:Evry/Protocols/03
    Add 600 μL of Buffer A4 (wash buffer) previously supplemented with ethanol. Centrifuge ar 11 000 x g for 1 minute. Discard flow-through and place the
    3 KB (532 words) - 13:25, 1 October 2013
  • Team:Evry/Protocols/04
    <p><b>Chloramphenicol:</b><br/> Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL).<br>
    6 KB (881 words) - 12:03, 6 September 2013
  • Team:Leeds/Results
    ...to gain further information in which cells were exposed to the stresses of ethanol, Triton X-100 and pH gradients. In this second experiment, a robotic pipett
    20 KB (3,155 words) - 03:58, 5 October 2013
  • Team:Macquarie Australia/Safety
    ... waste in biohazard bins, correct labelling of materials, disinfect (with ethanol) the benches both before and after use and adhere to safe laboratory practi
    6 KB (928 words) - 17:27, 27 September 2013
  • Team:Glendale CC AZ/Protocols/SurivalGrowth
    -20mL ethanol (to sterilize spreader)
    9 KB (1,009 words) - 02:34, 28 September 2013
  • Team:TU Darmstadt/materials/Stock solutions
    <li>ad. 40mL ethanol</li>
    7 KB (984 words) - 03:52, 5 October 2013
  • UIUC Illinois/Safety/Database
    *70% Ethanol- used to clean bench tops at the beginning and end of each day
    5 KB (801 words) - 19:47, 13 August 2013
  • Team:Warsaw/Cytotoxicity
    ...ls in a given phase of the cell cycle. Permeabilization of cells with cold ethanol and addition of propidium iodide (PI) prior to analysis, allows us to measu
    11 KB (1,658 words) - 00:11, 5 October 2013
  • Team:NU Kazakhstan/Yeast
    ...has several advantages, firstly, it is nonpathogenic, play a vital role in ethanol and baker’s yeast production, and then it is a eukaryotic organism and ca
    6 KB (889 words) - 15:58, 27 September 2013
  • Team:Tsinghua-E/Notebook
    ...7H2O 16.1g/L, NaH2PO4.H2O 5.5g/L, KCl 0.75g/L, MgSO4.7H2O 0.25g/L, 2-thiol ethanol 2.7ml/L, adjusted pH to 7.0). Nextly, mix 2mL mixture with 250uL 1mg/mL MUG
    26 KB (4,157 words) - 17:51, 27 September 2013
  • Team:Imperial College/Industrial Implementation
    ...eveloped that we can utilise this in our system as they already do in fuel ethanol production.
    13 KB (2,110 words) - 03:35, 29 October 2013
  • Team:Paris Saclay/Notebook/August/14
    We obtained low quantities of plasmid that's why we will make an Ethanol precipitation to concentrate it.
    8 KB (1,252 words) - 01:29, 5 October 2013
  • Team:KAIST Korea/Safety
    ...s and lab coats in the lab which are sterilized frequently by spraying 70% ethanol. When treating toxic and volatile reagents, such as TEMED (the reagent used
    33 KB (4,370 words) - 08:45, 23 August 2013
  • Team:Freiburg/Notebook/method
    ...Promega vacuum "Sau", 6ml buffer amounts used, columns were not dried from ethanol before eluting. Some columns were clogged and had to be scraped free with s
    69 KB (10,135 words) - 19:50, 28 October 2013
  • Team:BYU Provo/Notebook/Cholera - Enzyme/September/Period1/Dailylog
    ...w through liquid and centrifuge again for 1 to 2 minutes, to remove excess ethanol.
    7 KB (1,180 words) - 21:09, 27 September 2013
  • Team:UCL/Team/Profile
    ...njoy picnics, travelling and have an unfathomable passion for food and 70% ethanol. </p>
    8 KB (1,161 words) - 03:58, 5 October 2013
  • Team:Evry/Protocols/11
    Add 700 µL of Wash Buffer (previously dilted with ethanol) to the purification column. <br>
    3 KB (531 words) - 09:55, 2 September 2013
  • Team:TU-Delft/Notebook/2013/08/27/
    5. CIP treatment and Ethanol purification was carried out on pBAD:receiver. <br>
    1 KB (190 words) - 09:00, 4 October 2013
  • Team:Heidelberg/Templates/Methods
    * add 4 volumes 70% ethanol
    32 KB (4,951 words) - 01:39, 29 October 2013
  • Team:Goettingen/NoteBook w13
    ...nbsp; &nbsp; &nbsp; &nbsp; &nbsp; </span><span>clean bench and pipets with ethanol</span></p><p class="c13-30 c13-3"><span class="c13-15">-</span><span class=
    134 KB (22,952 words) - 21:14, 30 September 2013
  • Team:Bielefeld-Germany/Labjournal/Materials
    <p>-Solubilize 20 mg mL-1 Chloramphenicol in 100 % Ethanol </p>
    8 KB (1,303 words) - 11:07, 26 September 2013
  • Team:BYU Provo/Notebook/Cholera - Detection/Winterexp/Period2/Dailylog
    ...1ml of saturated culture for the initial step. Also we could have done the ethanol wash spin down for longer. Submitted 2 samples for sequencing using the m
    16 KB (2,963 words) - 22:59, 27 September 2013
  • Team:Washington/POURING AGAR PLATES
    2. Prep plating area: sterilize surface with ethanol, turn bunsen burner on <br />
    25 KB (3,003 words) - 07:19, 7 September 2013
  • Team:Washington/NANODROP PROTOCOL
    ...vely, you can clean the sample surfaces with a Kimwipe moistened with 70% ethanol. </li>
    25 KB (2,953 words) - 07:20, 7 September 2013
  • Team:NYMU-Taipei/Navigation
    ... <a href="https://2013.igem.org/Team:NYMU-Taipei/Modeling/Ethanol">Ethanol Model</a>
    11 KB (1,272 words) - 02:50, 29 October 2013
  • Team:Heidelberg/Tyrocidine week19 biobrickmod
    * Mixture was purified with isoprop, washed with ethanol and eluted in 10µl H2O
    3 KB (459 words) - 16:59, 4 October 2013
  • Team:WHU-China/templates/standardpage safety
    &nbsp; &nbsp; &nbsp; &nbsp;NaOH, HCl, SDS, acrylamide, TEMED, ethanol, IPTG, Restrictive enzymes from NEB, GelRed, Agarose, TAE buffer, 10XLoadin
    10 KB (1,469 words) - 02:39, 26 September 2013
  • Team:Evry/Pill design
    ...medicine to the duodenum/jejunum. To this end, we soaked the capsule in an ethanol-based solution of methacrylic acid and dried it with hot air. This method p
    21 KB (3,061 words) - 03:07, 29 October 2013
  • Team:BIT/project microchip
    ...ome plate. The Optical Adhesive that was not solidified will be removed by Ethanol - acetone solution (volume ratio 8:2) and dried by a drier. <br>
    12 KB (1,651 words) - 02:27, 28 October 2013
  • Team:Goettingen/NoteBook w6
    buffer A4 without Ethanol added… concentrations after elution were quite low…<o:p></o:p></span></
    387 KB (51,437 words) - 21:20, 30 September 2013
  • Team:Calgary/Sandbox/Notebook/Protocols/PCRPurification
    ...iBind DNA column by adding 700µl of DNA Wash Buffer diluted with absolute ethanol and centrifuge centrifuge at 10,000x g for 1 min at room temperature</li>
    2 KB (275 words) - 05:48, 18 September 2013
  • Team:Calgary/Sandbox/Notebook/Protocols/GelExtraction
    <li>Wash the column with 700 µL of SPW buffer with added ethanol and spin down at 10,000xg for 1 min</li>
    2 KB (318 words) - 05:51, 18 September 2013
  • Team:Goettingen/NoteBook w11
    ...ezer box – 20 °C</span></p><p class="c11-0"><span>&nbsp;(calculation of ethanol that has to be added to a certain amount of Cm:</span></p><p class="c11-0">
    105 KB (20,098 words) - 21:18, 30 September 2013
  • Team:Hong Kong HKUST/notebook/mod3
    Ethanol precipitation of pEGFP-N1 (4th) (using remaining 3rd EGFP plasmid) <br />
    26 KB (4,151 words) - 23:14, 27 September 2013
  • Team:TzuChiU Formosa/Protocol
    <li>Add 1 volume 95% ethanol, and put it on room temperature for 20 min.
    14 KB (1,938 words) - 00:01, 28 September 2013

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