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Team:Paris Saclay/ethanol
= '''Ethanol precipitation''' = 2. Add 3x volumes of ethanol 100%

741 B (113 words) - 14:23, 4 October 2013
Team:NYMU-Taipei/Modeling/Ethanol
=Ethanol model= ...ter are enzyme PDC and ADH-producing genes, which will convert pyruvate to ethanol, and thus kill spores of ''Nosema Ceranae''.

10 KB (1,535 words) - 02:39, 29 October 2013
Team:NYMU-Taipei/Modeling/Ethanol/explanation
\frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.

6 KB (928 words) - 14:09, 27 October 2013

Page text matches

Team:Edinburgh/Project/Notebook
...f the culture). Those were inoculated with 1ml of differently concentrated ethanol solutions giving final concentrations of: ...ompetent ''E. coli'' cells were tested by transforming them with parts for ethanol production. The negative control was performed to confirm that cells have n

41 KB (6,583 words) - 22:47, 4 October 2013
Team:NYMU-Taipei/Project/Kill
*Ethanol is known as a kind of toxins to bees. Up to certain degree, it has potentia *Ethanol can be produced from pyruvate through [http://parts.igem.org/Part:BBa_K110

17 KB (2,669 words) - 03:06, 29 October 2013
Team:NYMU-Taipei/Modeling/Ethanol
=Ethanol model= ...ter are enzyme PDC and ADH-producing genes, which will convert pyruvate to ethanol, and thus kill spores of ''Nosema Ceranae''.

10 KB (1,535 words) - 02:39, 29 October 2013
Team:Concordia/Notebook
<h2 class="log-title">Ethanol purification of EnvZ, and Fusions DOM/CON</h2> ... followed by 70% ethanol and re-suspension in Ultra Pure Distilled water. Ethanol Purification products were then verified and quantified using a 1% agarose

16 KB (2,286 words) - 02:48, 28 September 2013
Team:Edinburgh/Project/Results/Bioethanol Results
<h3>Ethanol production using fused pdc and adhB:</h3> Pdc and adhB catalyse conversion of pyruvate to ethanol via acetaldehyde intermediate.

8 KB (1,244 words) - 01:06, 5 October 2013
Team:USP-Brazil/Results:FreezeDry
<h4>Ethanol Resistance after Lyophilization</h4> ...od for our intended application, where the yeast will be resuspended using ethanol solution

9 KB (1,304 words) - 04:02, 28 September 2013
Team:USP-Brazil/Project
...on, P.pastoris cultures are able to grow in media with up to 10% of ethanol [10], which makes it a perfect candidate for our chassis. ... means methanol will not be degraded as fast as it would in the absence of ethanol. Therefore, the degradation of peroxisomes would enhance the activation of

18 KB (2,818 words) - 03:33, 28 September 2013
Team:USP-Brazil/Model:Deterministic
a report protein RFP with different concentrations of ethanol and methanol. By making some hypothesis, we ...xpression of the promoter in the presence of other sources of carbon, like ethanol. Because of

10 KB (1,556 words) - 04:17, 28 September 2013
Team:KIT-Kyoto/Notebook/ATF2
was purified by ethanol precipitation was purified by ethanol precipitation.

110 KB (16,118 words) - 02:26, 28 September 2013
Team:USP-Brazil/Results:Characterization
<h4>Chassis characterization, behavior of Pichia pastoris growth on ethanol solutions</h4> ...ity to different ethanol concentrations, this help us to define a specific ethanol range for input testing.

5 KB (812 words) - 01:20, 28 September 2013
Team:RHIT/Protocol.html
• Add ethanol (96-100%) to Buffer PE before use. 3. Add 2x current volume ice cold ethanol.

259 KB (27,268 words) - 01:38, 28 September 2013
Team:Toronto/Notebook
assays with ethanol, temperature and salt stimuli on clear plates Amount of ethanol that needs to be pipetted for each stimulus treatment was

30 KB (4,922 words) - 03:11, 28 September 2013
Team:NJU China/Protocol
75% Ethanol (in DEPC-treated water) ...nt. Wash the RNA pellet once with 75% ethanol, adding at least 1 ml of 75% ethanol per 1 ml of TRIZOL Reagent used for the initial homogenization. Mix the sam

69 KB (9,899 words) - 08:26, 28 October 2013
Team:NJU China/test
75% Ethanol (in DEPC-treated water) ...nt. Wash the RNA pellet once with 75% ethanol, adding at least 1 ml of 75% ethanol per 1 ml of TRIZOL Reagent used for the initial homogenization. Mix the sam

72 KB (10,248 words) - 02:00, 28 September 2013
Team:Edinburgh/Modeling/Engineering model
...e-settling, biofilm formation and filtration, reverse osmosis sections and ethanol conversion. Ideally, all these four sections will be able to accomplish tas ...t the surface of the water by a skimming device, which is connected to the ethanol conversion section for biofilm production. Most, not necessarily all, insol

9 KB (1,405 words) - 03:23, 5 October 2013
Team:USP-Brazil/LaBioTec
...ent ethanol concentration on media by performing a Pichia pastoris Ethanol Survival test protocol. To evaluate the viability of resuspended lyophilized samples in ethanol solutions 5 lyophilized samples were resuspended: 

15 KB (2,594 words) - 01:19, 28 September 2013
Team:USP-Brazil/Parts
...ed in the carbon source-dependent regulation of Mxr1, which is inactive in ethanol and glycerol, but is active in methanol. Our submitted Mxr1 is mutated in order to NOT be repressed by ethanol, as the original Mxr1 is. This is done by substituted the Ser215 of the pro

4 KB (613 words) - 00:42, 28 September 2013
Team:Toronto/elementss
increased in environments with 1% ethanol or NaCl. [9] Ethanol 

16 KB (2,473 words) - 00:48, 28 September 2013
Team:Edinburgh/Project/Appendix
'''Ethanol production''' Figure 3. Amount of ethanol produced following 24h aerobic incubation.

8 KB (1,169 words) - 21:49, 4 October 2013
Team:Toronto/Project/Elementss
increased in environments with 1% ethanol or NaCl. [9] Ethanol 

20 KB (3,131 words) - 03:41, 28 September 2013
Team:METU Turkey/notebook.html
Hood is cleaned by ethanol, sterilized by uv light and shutted down. ...en burner gases have been shutted down and checked, the bench cleaned with ethanol

68 KB (11,183 words) - 03:36, 5 October 2013
Team:Kyoto/Material and method
*Add 800µL 80% ethanol and vortex. ==Ethanol Precipitation==

15 KB (2,547 words) - 12:47, 10 October 2013
Team:Tianjin/Protocol
.... Wash the column by adding 600μl of PW Wash Buffer diluted with absolute ethanol. Centrifuge at 12,000 rpm for 1 min at room temp. Note: PW Wash Buffer Concentrate must be diluted with absolute ethanol before use. See label for directions. If refrigerated, PW Wash Buffer must

24 KB (3,678 words) - 21:20, 27 September 2013
Team:BIOINT Mexico/Lab work
*The supernatant was discarded and 500 µl of Wash Buffer with ethanol (w10) were added to the column *700 µl of Wash Buffer W9 with ethanol were added to the column

41 KB (6,916 words) - 04:05, 28 September 2013
Jamboree/Team Abstracts
...uld not only react to methanol, but also survive in high concentrations of ethanol. ... them with ease. Now all what it takes to finish the job is adding some ethanol to obtain our biodiesel!

182 KB (27,381 words) - 14:33, 28 October 2013
Team:Edinburgh/Introduction/Bioethanol
...iety of renewable, biomass-derived carbon sources compared to the standard ethanol producer: ''Sacharomyces cerevisiae''. ...ons catalysed by those enzymes (Figure 1) enable conversion of pyruvate to ethanol.

3 KB (455 words) - 01:30, 5 October 2013
Team:NYMU-Taipei/Modeling/Overview
...ates a sick bee timely when killing protein fails to kill Nosema, and that ethanol won’t kill a bee if it is cured in time. ...protein production and ethanol production more keenly. Killing protein and ethanol production effects an infected bee alternatively when the bee is in differe

4 KB (564 words) - 03:07, 29 October 2013
Read more20
\frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.

2 KB (226 words) - 02:22, 28 October 2013
Team:ITU MOBGAM Turkey/protocol
Ethanol precipitation of small fragments ...the sample is in a 1.5 mL eppendorf tube. I recommend storing the absolute ethanol at -20°C. 

15 KB (2,323 words) - 22:49, 4 October 2013
Team:NYMU-Taipei/Modeling/Ethanol/explanation
\frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.

6 KB (928 words) - 14:09, 27 October 2013
More
\frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.

6 KB (928 words) - 14:16, 27 October 2013
More7
\frac{d[ethanol]}{dt}=Kpyruvateacetaldehyde\times{Kacetaldehydeethanol}\times\frac{[PDC]^{n ...ant of ethanol→acetaldehyde, a reverse reaction of the second process of ethanol synthesis.

2 KB (226 words) - 00:46, 28 October 2013
Team:KU Leuven/Protocols
...1ng/ml of MeS (Sigma-Aldrich, ReagentPlus®, ≥99% (GC)) in 97% pure ethanol or water. ...with the desired concentration for 10 minutes. MeSA was first suspended in ethanol before being further diluted to the desired concentration in 50ml water

38 KB (6,008 words) - 03:58, 29 October 2013
Jsher
*Going to test Ethanol + NR with different amounts of ATC *Also going to repeat ATC and Ethanol experiment

64 KB (10,685 words) - 04:42, 16 January 2014
Team:NU Kazakhstan/Protocols
<li>Ethanol (100% and 75%)</li> <li>The elutes (1 and 2) were diluted with 200 μl H2O (each) before ethanol precipitation;</li>

14 KB (2,323 words) - 16:47, 25 September 2013
Team:USP-Brazil/Protocols
<li>Add 500 μL of ethanol 70%. Invert 5 times.</li> <h4>Ethanol Precipitation of Plasmid DNA</h4>

19 KB (3,066 words) - 02:55, 27 September 2013
Team:UC-Santa Cruz/Notebook3
Ethanol ...e’s 10% ethanol in lab if not we can make 10% ethanol by adding 100ml of ethanol to 900ml

27 KB (4,367 words) - 04:04, 28 September 2013
(click here for detailed information about circuit design)
We want the suicide part (ethanol) to be opened only after the first way (kill protein) fails; that is, Nosem ... time to go through the terminator barrier and thus, create a time lag for ethanol production.

1 KB (192 words) - 02:42, 28 October 2013
J Sher
*Going to test Ethanol + NR with different amounts of ATC *Also going to repeat ATC and Ethanol experiment

64 KB (10,685 words) - 04:40, 16 January 2014
Team:Newcastle/HP/Safety
===Ethanol=== ...e lab to wipe down surfaces and pipettes. The main problem associated with ethanol is irritation especially in the eyes. It may cause pain or permanent cornea

13 KB (2,157 words) - 21:10, 4 October 2013
Team:Evry/Protocols
Chloramphenicol: Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL). Add 400 μL of 50% ethanol and mix with a vortex or a pipette. 

44 KB (6,816 words) - 03:48, 5 October 2013
Team:Manchester/LabBookText
.../ml ampicillin agar plates mate. 0.75g of ampicillin added to 15 ml of 50% ethanol to create 50 mg/ml stock to be kept in -20 ºC freezer. 0.4ml of this stock Want a 34 mg/ml stock in 100% ethanol: 0.34 g in 10 ml of ethanol

26 KB (4,304 words) - 12:39, 30 September 2013
Team:Manchester/LabBooktest
.../ml ampicillin agar plates mate. 0.75g of ampicillin added to 15 ml of 50% ethanol to create 50 mg/ml stock to be kept in -20 ºC freezer. 0.4ml of this stock Want a 34 mg/ml stock in 100% ethanol: 0.34 g in 10 ml of ethanol

60 KB (8,597 words) - 13:33, 26 October 2013
Team:Evry/Protocols/
Chloramphenicol: Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL). Add 400 μL of 50% ethanol and mix with a vortex or a pipette. 

44 KB (6,791 words) - 02:37, 5 October 2013
Team:Evry/Protocols/Test
Chloramphenicol: Put 525 mg of Kanamycin in 15 mL of ethanol (35 mg per mL). Add 400 μL of 50% ethanol and mix with a vortex or a pipette. 

44 KB (6,791 words) - 02:35, 5 October 2013
Team:Manchester/test4
.../ml ampicillin agar plates mate. 0.75g of ampicillin added to 15 ml of 50% ethanol to create 50 mg/ml stock to be kept in -20 ºC freezer. 0.4ml of this stock Want a 34 mg/ml stock in 100% ethanol: 0.34 g in 10 ml of ethanol

59 KB (8,384 words) - 11:41, 2 October 2013
Team:Manchester/LabBook
.../ml ampicillin agar plates mate. 0.75g of ampicillin added to 15 ml of 50% ethanol to create 50 mg/ml stock to be kept in -20 ºC freezer. 0.4ml of this stock Want a 34 mg/ml stock in 100% ethanol: 0.34 g in 10 ml of ethanol

62 KB (8,850 words) - 13:33, 26 October 2013
Team:Newcastle/Notebook/protocols
#Add 200 µl ethanol to the tube and vortex briefly. *Add ethanol (96-100%) to Buffer PE before use (see bottle label for volume).

20 KB (3,259 words) - 20:49, 4 October 2013
Team:Stanford-Brown/Projects/BioWires
... and 22µl of 3M CH3COONa was added to each tube (10% of final volume) for ethanol precipitation. Then 467 µl of isopropanol was added to each tube (70% of f ...econds each). It was then destained by dipping into another droplet of 50% ethanol for 5 seconds. The sample was dried again by wicking. Negative staining sam

33 KB (5,289 words) - 05:36, 13 November 2013
Team:Paris Bettencourt/Protocols
<li>Add 500 ul of the Wash Solution (diluted with ethanol) to the GeneJET spin column. Centrifuge for 30-60 seconds and discard the f ...to remove residual Wash Solution. This step is essential to avoid residual ethanol in plasmid preps.

29 KB (4,517 words) - 15:19, 16 October 2013