Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 Mutagen Concentration Experiment
From 2013.igem.org
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: - The stock solutions are now stored in 4C | : - The stock solutions are now stored in 4C | ||
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+ | 2) Spot test (5.22) | ||
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+ | : - Spot test was performed following procedures similar to those in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU Provo/5.3 Phage Amplification/Purification|5.3 T7 Phage Amplification/Purification]] | ||
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+ | 3) Preliminary selection test (5.24) | ||
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+ | : - Preliminary selection test was performed using x8 agar; -6, -7, and -8 dilutions; and procedure similar to those in [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 T7 phage selection method test|5.3 T7 Phage Selection Method Test]] | ||
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+ | '''V) Results''' | ||
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+ | 1) Applying mutagen | ||
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+ | : Phage did not amplify in the liquid culture with 5-bromodeoxyuridine. Calculations revealed that the initial concentration of phage in liquid culture should be approximately 2.5E7 phage particle/mL. This is on the same scale as the final phage concentration as revealed by the spot test below. | ||
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+ | [[File:MutagenTestTube.JPG|400px|center]] | ||
2) Spot test | 2) Spot test | ||
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+ | : Lower concentration of mutagen seen to stimulate phage production, while higher concentration inhibits | ||
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+ | [[File:MutagenPlate1.JPG|400px|left]] [[File:MutagenPlate2.JPG|400px|right]] | ||
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+ | 3) Preliminary selection test | ||
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+ | : Only one or two plaques are seen on each plate. | ||
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+ | [[File:MutagenPlate3.JPG|400px|center]] | ||
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+ | '''VI) Conclusion''' | ||
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+ | ADD | ||
Revision as of 21:16, 27 May 2013
Small Phage Spring Notebook: Experiments
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5.20 Mutagen Concentration Experiment
I) Purpose
II) Expected Outcome
III) Reagents Used
IV) Procedure 1) Applying the mutagen (5.20)
2) Spot test (5.22)
3) Preliminary selection test (5.24)
V) Results 1) Applying mutagen
2) Spot test
3) Preliminary selection test
VI) Conclusion ADD
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