Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.14 Mutagen Concentration Test - Seventh Protocol
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* Label 3 test tubes 0, 200, 500. Add 9.8mL of LB and 40ul of adenine solution into each test tube. Then add 200ul of BL21 overnight into each test tube. Incubate on the shaker at 37 Celsius. | * Label 3 test tubes 0, 200, 500. Add 9.8mL of LB and 40ul of adenine solution into each test tube. Then add 200ul of BL21 overnight into each test tube. Incubate on the shaker at 37 Celsius. | ||
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* Remove all the test tubes off the shaker after 2 hours. Add 40ul of adenine and 80ul of uracil to each test tube. Also add the corresponding amount in ul of 5-bromodeoxyuridine, a mutagen, to each test tube. (Ex: Add 200ul of mutagen to tube labeled 200) | * Remove all the test tubes off the shaker after 2 hours. Add 40ul of adenine and 80ul of uracil to each test tube. Also add the corresponding amount in ul of 5-bromodeoxyuridine, a mutagen, to each test tube. (Ex: Add 200ul of mutagen to tube labeled 200) |
Revision as of 18:32, 14 August 2013
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8.14 Mutagen Concentration Test - Seventh Protocol
I) Purpose To mutate T7 phage for different capsid sizes. II) Expected Outcome
III) Reagents Used
IV) Procedure 1) Overnight (The day before) (8.13)
2) Applying the mutagen (7.29)
3) Spot test to determine phage concentration
2) Applying the mutagen
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