Team:Bielefeld-Germany/Labjournal

From 2013.igem.org

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*GldA BioBrick (<bbpart>BBa_K1172201</bbpart>) was examined.
*GldA BioBrick (<bbpart>BBa_K1172201</bbpart>) was examined.
**GldA Biobrick Devices with different promotors and RBS could be added: (<bbpart>BBa_K1172203</bbpart>, <bbpart>BBa_K1172204</bbpart>, <bbpart>BBa_K1172205</bbpart>)
**GldA Biobrick Devices with different promotors and RBS could be added: (<bbpart>BBa_K1172203</bbpart>, <bbpart>BBa_K1172204</bbpart>, <bbpart>BBa_K1172205</bbpart>)
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*OprF BioBrick <bbpart>BBa_K1172501</bbpart> is available.
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*OprF BioBrick (<bbpart>BBa_K1172501</bbpart>) is available.
**OprF Biobrick was functionalized with different promotors and RBS: (<bbpart>BBa_K1172502</bbpart>, <bbpart>BBa_K1172503</bbpart>, <bbpart>BBa_K1172504</bbpart>, <bbpart>BBa_K1172505</bbpart>, <bbpart>BBa_K1172507</bbpart>)
**OprF Biobrick was functionalized with different promotors and RBS: (<bbpart>BBa_K1172502</bbpart>, <bbpart>BBa_K1172503</bbpart>, <bbpart>BBa_K1172504</bbpart>, <bbpart>BBa_K1172505</bbpart>, <bbpart>BBa_K1172507</bbpart>)
*Successful protein expression and overproduction of glycerol dehydrogenase. SDS-PAGE shows GldA at expected size.
*Successful protein expression and overproduction of glycerol dehydrogenase. SDS-PAGE shows GldA at expected size.

Revision as of 09:26, 3 October 2013



Journal



Milestones





Overview Prologue

  • The iGEM-Team Bielefeld 2013 started working and decided to deal with the project 'Microbial Fuel Cell'.


Overview May

  • Initiating labwork on the sub-project endogenous mediator Glycerol dehydrogenase.
  • Starting lab work with the first successful PCR's. The main work however is still in the planning of our project. Designing experiments and a lot of research.
  • Finding sponsors goes ahead. Many companies like our project and want to support us.


Overview June

  • Starting labwork on the sub-project Porins.
  • Successful PCR on OprF gene from Pseudomonas fluorescens. OprF with Pre- and Suffix overlaps could be amplified from genome.
  • Planning of our Human Practice projects started and the first participations are fixed.


Overview July

  • Poster presentation at the congress ‘[http://www.biotechnologie2020plus.de Next generation of biotechnological processes 2020+]’ in Berlin.
  • Sponsoring acquisition is nearly finished.
  • Problem with cloning delaying the progress in the laboratory.
  • We have published our [http://ekvv.uni-bielefeld.de/blog/uniaktuell/entry/mit_bakterien_batterie_strom_erzeugen first press release] with a great response. The press release was picked up worldwide.


Overview August

  • Our great expert Dr. Falk Harnisch has answered numerous questions about our project and helped us very well.
  • GldA BioBrick (<bbpart>BBa_K1172201</bbpart>) was examined.
    • GldA Biobrick Devices with different promotors and RBS could be added: (<bbpart>BBa_K1172203</bbpart>, <bbpart>BBa_K1172204</bbpart>, <bbpart>BBa_K1172205</bbpart>)
  • OprF BioBrick (<bbpart>BBa_K1172501</bbpart>) is available.
    • OprF Biobrick was functionalized with different promotors and RBS: (<bbpart>BBa_K1172502</bbpart>, <bbpart>BBa_K1172503</bbpart>, <bbpart>BBa_K1172504</bbpart>, <bbpart>BBa_K1172505</bbpart>, <bbpart>BBa_K1172507</bbpart>)
  • Successful protein expression and overproduction of glycerol dehydrogenase. SDS-PAGE shows GldA at expected size.


Overview September


Overview October



Contents