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Revision as of 23:05, 6 August 2013 (view source) Sgrenz (Talk | contribs) ← Older edit		Revision as of 23:06, 6 August 2013 (view source) Sgrenz (Talk | contribs) Newer edit →
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		+	<br>
		+	zu beachten ist blablbalbalbalaba
	</tr>		</tr>

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Revision as of 23:06, 6 August 2013

PCR Protocols and Programs

Phusion 25 µl

Reagenz	1x	8x
Buffer 5x	5,0	44
MgSO4	0	0
dNTPs	2,5	22
Primer1	1,0	8,8
Primer2	1,0	8,8
Template	1,5	13,2
DMSO	2,0	17,6
Polymerase	0,5	4,4
H2O	11,5	101,2

Program Phusion
98 °C	98 °C	x °C	72 °C	72 °C
2 min	30 s	30 s	y	6 min
x: according to Primer Tm y: 15s/kb Notes zu beachten ist blablbalbalbalaba

Taq 25 µl

Reagenz	1x	8x
Buffer 5x	5,0	44
MgSO4	0	0
dNTPs	2,5	22
Primer1	1,0	8,8
Primer2	1,0	8,8
Template	1,5	13,2
DMSO	2,0	17,6
Polymerase	0,5	4,4
H2O	11,5	101,2

Program Taq
98 °C	98 °C	x °C	72 °C	72 °C
2 min	30 s	30 s	y	6 min
x: according to Primer Tm y: 15s/kb