October

Milestones

Week 22

Organization

• Wiki freeze is coming on saturday at 5:59 pm. Plannung the last week with tremendous amount of work.

MFC

Mediators

• For testing the genetic engineered system in the Microbial Fuel Cell, we used Escherichia coli KRX with GldA and Lac promotor (<bbpart>BBa_K1172204</bbpart>) in contrast to Escherichia coli KRX Wildtyp. Certainly NADH-assays determined Escherichia coli KRX with GldA and T7 promotor (<bbpart>BBa_K1172203</bbpart>) as the best endogenous mediator producing strain. Unfortunately we could not use this strain due to cultivation problems.

Figure 6: Microbial Fuel Cell results from cultivation of Escherichia coli KRX with GldA (<bbpart>BBa_K1172204</bbpart>) in contrast to Escherichia coli KRX Wildtyp. Voltage curve from Escherichia coli KRX Wildtyp and Escherichia coli KRX with GldA (<bbpart>BBa_K1172204</bbpart>) is shown over time. M9-medium was used with no supplementation of mediators.

Figure 7: Microbial Fuel Cell results from cultivation of Escherichia coli KRX with GldA (<bbpart>BBa_K1172204</bbpart>) in contrast to Escherichia coli KRX Wildtyp. Electric charge curve from Escherichia coli KRX Wildtyp and Escherichia coli KRX with GldA (<bbpart>BBa_K1172204</bbpart>) is shown over time. M9-medium was used with no supplementation of mediators.

• The extracellular electron transfer mediated by NADH is improved in the GldA strain resulting in an increased bioelectricity output.

Cytochromes

Biosafety

Porines

• Great results for Escherichia coli KRX with OprF (<bbpart>BBa_K1172502</bbpart>) in contrast to Escherichia coli KRX Wildtyp with Microbial Fuel Cell cultivation.
• According to our assumptions, the extracellular electron transfer mediated by electron shuttles is improved in the OprF strain resulting in an increased bioelectricity output. (Figure 1 and 2)

Figure 1: Microbial Fuel Cell results from cultivation of Escherichia coli KRX with OprF (<bbpart>BBa_K1172502</bbpart>) in contrast to Escherichia coli KRX Wildtyp. Voltage curve from Escherichia coli KRX Wildtyp, Escherichia coli KRX with OprF (<bbpart>BBa_K1172502</bbpart>), M9 medium with used mediator New Methylene Blue and M9 medium without mediator is shown over time.

Figure 2: Microbial Fuel Cell results from cultivation of Escherichia coli KRX with OprF (<bbpart>BBa_K1172502</bbpart>) in contrast to Escherichia coli KRX Wildtyp. Electric charge curve from Escherichia coli KRX Wildtyp and Escherichia coli KRX with OprF (<bbpart>BBa_K1172502</bbpart>). M9 medium was used with mediator New Methylene Blue.

• Escherichia coli KRX with OprF (<bbpart>BBa_K1172502</bbpart>) shows 100 % higher voltage and electric charge than E. coli Wildtyp.

Week 23

Organization

MFC

Mediators

Cytochromes

Biosafety

Porines

Week 24

Organization

MFC

Mediators

Cytochromes

Biosafety

Porines

Week 25

Organization

MFC

Mediators

Cytochromes

Biosafety

Porines