Team:Bielefeld-Germany/SinceRegionals

From 2013.igem.org

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===Genetic Optimization===
===Genetic Optimization===
*<p align="justify">In order to test the effect of the protein expression of the [https://2013.igem.org/Team:Bielefeld-Germany/Project/Porins#Growth_characteristics outer membrane porin OprF] and the [https://2013.igem.org/Team:Bielefeld-Germany/Project/GldA#Growth_characteristics glycerol dehydrogenase GldA] on the cell growth, growth was measured as increase in optical density and plotted against time.</p>
*<p align="justify">In order to test the effect of the protein expression of the [https://2013.igem.org/Team:Bielefeld-Germany/Project/Porins#Growth_characteristics outer membrane porin OprF] and the [https://2013.igem.org/Team:Bielefeld-Germany/Project/GldA#Growth_characteristics glycerol dehydrogenase GldA] on the cell growth, growth was measured as increase in optical density and plotted against time.</p>
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<bbpart>BBa_K1172502</bbpart>
 
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Revision as of 13:31, 26 October 2013



Since Regionals


Overview

Since the European Jamboree in Lyon we have been busy and continued working on our project. We managed to create serveral devices by different combinations of the mediator producing parts and the outer membrane porin, did some modelling on the maximal yield of glycerol as a carbon source and conducted additional measurements with our MFC. We even printed a car that should be powered by our MFC.

Genetic Optimization


  • We constructed new devices by combination of the mediator producing parts (riboflavin synthesis gene cluster and glycerol dehydrogenase) with the outer membrane porin OprF.

    1. <bbpart>BBa_K1172566</bbpart> - OprF and GldA (Coding): Combination of the outer membrane porin OprF(<bbpart>BBa_K1172501</bbpart>) and the glycerol dehydrogenase GldA (<bbpart>BBa_K1172201</bbpart>).
    2. <bbpart>BBa_K1172577</bbpart> - OprF and GldA (Coding): Combination of the outer membrane porin OprF (<bbpart>BBa_K1172502</bbpart>) and the glycerol dehydrogenase GldA (<bbpart>BBa_K1172201</bbpart>) under control of T7 promoter.
    3. <bbpart>BBa_K1172588</bbpart> - OprF and Riboflavin synthesis gene cluster (Coding): Combination of the outer membrane porin OprF (<bbpart>BBa_K1172501</bbpart>) and Riboflavin synthesis gene cluster (BBa_K1172303).
    4. <bbpart>BBa_K1172599</bbpart> - OprF and Riboflavin synthesis gene cluster (Coding): Combination of the outer membrane porin OprF (<bbpart>BBa_K1172502</bbpart>) and the Riboflavin synthesis gene cluster (BBa_K1172303) under control of T7 promoter.


Engineering Optimization

Human Practice





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Contents


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