Team:DTU-Denmark/Methods

From 2013.igem.org

(Difference between revisions)
(Plasmid isolation)
(Other)
 
(25 intermediate revisions not shown)
Line 17: Line 17:
[[Team:DTU-Denmark/Methods/Colony_PCR|Colony PCR]]
[[Team:DTU-Denmark/Methods/Colony_PCR|Colony PCR]]
 +
 +
 +
==USER Cloning==
 +
[[Team:DTU-Denmark/Methods/USER_Cloning_and_Transformation|USER Cloning and Transformation]]
==Plasmid isolation==
==Plasmid isolation==
Line 22: Line 26:
[[Team:DTU-Denmark/Methods/Plasmid_isolation|Plasmid isolation by ethanol precipitation]]
[[Team:DTU-Denmark/Methods/Plasmid_isolation|Plasmid isolation by ethanol precipitation]]
 +
==Determining concentration of nitrogen compounds==
 +
 +
[[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_1a|Experiment 1a]] -- Toxicity of Nitrite, Nitrate and Ammonium to ''E. coli''
 +
 +
[[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_2|Experiment 2]] -- Anaerobic production of nitrous oxide from nitrite
 +
 +
[[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_4|Experiment 4]] -- Aerobic consumption of ammonium and hydroxylamine
 +
 +
==Other==
 +
 +
[[Team:DTU-Denmark/Methods/Visualizing_GFP_in_the_periplasm|Visualizing GFP in the periplasm]]
 +
 +
[[Team:DTU-Denmark/Methods/chemically_compentent_cells|Preparation of chemically competent cells]]
 +
 +
[[Team:DTU-Denmark/Methods/Autoclaving|Autoclaving]]
 +
 +
[[Team:DTU-Denmark/Methods/Making anoxic conditions|Sparging with N<sub>2</sub>]]
 +
 +
[[Team:DTU-Denmark/Methods/Modified DM minimal medium|Modified DM minimal medium]]
 +
 +
[[Team:DTU-Denmark/Methods/Calibrating Electrodes|Calibration of Electrodes]]
 +
 +
[[Team:DTU-Denmark/Methods/Ammonium colorimetric measurements|Ammonium colorimetric measurements]]
 +
 +
[[Team:DTU-Denmark/Methods/Nitrate colorimetric measurements|Nitrate colorimetric measurements]]
 +
 +
[[Team:DTU-Denmark/Methods/Nitrite colorimetric measurements|Nitrite colorimetric measurements]]
 +
 +
[[Team:DTU-Denmark/Methods/rehydrating_primers|Rehydrating Primers]]
 +
 +
[[Team:DTU-Denmark/Methods/dpni_treatment|DpnI treatment]]
 +
[[Team:DTU-Denmark/Methods/Bioreactor|Bioreactor]]
 +
<div style="clear: both;"></div>
{{:Team:DTU-Denmark/Templates/EndPage}}
{{:Team:DTU-Denmark/Templates/EndPage}}

Latest revision as of 12:31, 1 October 2013

Methods

Contents


PCR

PCR

PCR-mix

PCR-ramp

PCR-touchdown

PCR-touchUp

PCR-gradient

Colony PCR


USER Cloning

USER Cloning and Transformation

Plasmid isolation

Plasmid isolation by ethanol precipitation

Determining concentration of nitrogen compounds

Experiment 1a -- Toxicity of Nitrite, Nitrate and Ammonium to E. coli

Experiment 2 -- Anaerobic production of nitrous oxide from nitrite

Experiment 4 -- Aerobic consumption of ammonium and hydroxylamine

Other

Visualizing GFP in the periplasm

Preparation of chemically competent cells

Autoclaving

Sparging with N2

Modified DM minimal medium

Calibration of Electrodes

Ammonium colorimetric measurements

Nitrate colorimetric measurements

Nitrite colorimetric measurements

Rehydrating Primers

DpnI treatment

Bioreactor