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{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
| - | + | <font size = "4"> Cell Surface Display </font> <BR> | |
| - | '''Making a glycerol | + | ''Moving on'' |
| - | We made a 50% glycerol stock of the Bacteria that we | + | <br>-Miniprep of cells transformed with ''INP_Strep-Dead'' gibson product. |
| + | <br> -DpnI digestion of Streptavidin iGEM (BBa_K283010) PCR product. | ||
| + | <br> -Gibson assembly of INP amplified construct and streptavidin iGEM PCR purified product. | ||
| + | <br> -Plating of Streptavdin alive received from Oxford University. | ||
| + | |||
| + | |||
| + | <font size = "4"> Sensing-Effector</font> <BR> | ||
| + | |||
| + | ''Making a glycerol Stock'' <BR> | ||
| + | -We made a 50% glycerol stock of the Bacteria that we inoculated the day before. | ||
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Cell Surface Display
Moving on
-Miniprep of cells transformed with INP_Strep-Dead gibson product.
-DpnI digestion of Streptavidin iGEM (BBa_K283010) PCR product.
-Gibson assembly of INP amplified construct and streptavidin iGEM PCR purified product.
-Plating of Streptavdin alive received from Oxford University.
Sensing-Effector
Making a glycerol Stock
-We made a 50% glycerol stock of the Bacteria that we inoculated the day before.
"
