Team:EPF Lausanne/Calendar/15 August 2013

From 2013.igem.org

(Difference between revisions)
(Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Primer Resuspension''' <BR> We recieved our primers and resuspended them. Then we dilluted them in order to have a final concentrati...")
 
(7 intermediate revisions not shown)
Line 1: Line 1:
{{Template:EPFL2013Header}}
{{Template:EPFL2013Header}}
 +
<font size = "4"> Cell Surface Display </font> <BR>
-
Sensing <BR>
+
''BBa_K523013 Characterisation'' <br>
 +
Characterisation of the iGEM plasmid BBa_K523013 we decided to use.
 +
<br><br>
-
'''Primer Resuspension''' <BR>
+
<font size = "4"> Sensing-Effector </font> <BR>
-
We recieved our primers and resuspended them. Then we dilluted them in order to have a final concentration of 100umol.
+
 +
''Primer Resuspension'' <BR>
 +
-We received our primers and resuspended them. Then we diluted them in order to have a final concentration of 100umol.
 +
<BR>
 +
 +
''Inoculating Bacteria'' <BR>
 +
-From each agar plate that we incubated over-night we inoculated one colony in order to make a glycerol stock of MMP2 and MMP9 containing bacteria the next day.
 +
<BR> <BR>
 +
 +
<font size = "4"> Nanoparicles</font> <BR>
 +
 +
''Making Nanoparticles, 2nd try''
 +
<BR>- The nanoparticles are done.
 +
<BR>- Characterisation of the new gelatin nanoparticles with the DLS : their average size is 160 nm !
{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Latest revision as of 21:51, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

BBa_K523013 Characterisation
Characterisation of the iGEM plasmid BBa_K523013 we decided to use.

Sensing-Effector

Primer Resuspension
-We received our primers and resuspended them. Then we diluted them in order to have a final concentration of 100umol.

Inoculating Bacteria
-From each agar plate that we incubated over-night we inoculated one colony in order to make a glycerol stock of MMP2 and MMP9 containing bacteria the next day.

Nanoparicles

Making Nanoparticles, 2nd try
- The nanoparticles are done.
- Characterisation of the new gelatin nanoparticles with the DLS : their average size is 160 nm !