Team:EPF Lausanne/Calendar/24 July 2013

From 2013.igem.org

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'''Cell surface Display'''
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<font size = "4">Cell Surface Display</font> <BR>
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Our Primers arrived today. <BR>
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''PCR'' <BR>
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We did a PCR (see protocols) to amplify ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction.
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We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised.
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<BR><BR>
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<font size = "4">General</font>
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Competent cells try 2.
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''Primers arrived:'' we did a PCR to amplifay ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction.
 
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We thus dis a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised.
 
{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Latest revision as of 21:23, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display
Our Primers arrived today.

PCR
We did a PCR (see protocols) to amplify pINP_construct to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised.

General

Competent cells try 2.