Team:EPF Lausanne/Calendar/25 July 2013

From 2013.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 1: Line 1:
{{Template:EPFL2013Header}}
{{Template:EPFL2013Header}}
-
'''Cell Surface Display'''
+
<font size = "4">Cell Surface Display</font>
-
''PCR optimisation:'' to improve the ''pINP_construct'' amplification we did a gradient PCR at 72, 73 and 74°C with 3% and 5% DMSO added to the mix.  
+
''PCR optimisation'' <BR>
-
we verified our results with a 0.8% gel electrophoresis.
+
To improve the ''pINP_construct'' amplification, we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix. We verified our results with a 0.8% gel electrophoresis.
 +
<BR><BR>
-
'''General'''
+
<font size = "4">General</font>
-
 
+
<BR>Competent cells try 3: it worked!  
-
Competent cells try 3: it worked!  
+
{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Latest revision as of 21:24, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

PCR optimisation
To improve the pINP_construct amplification, we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix. We verified our results with a 0.8% gel electrophoresis.

General
Competent cells try 3: it worked!