Team:EPF Lausanne/Calendar/27 August 2013

From 2013.igem.org

(Difference between revisions)
(Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Primer design''' <BR> We desinged new primers for the three sensing construct as well as for the three effector constructs. This tim...")
 
(4 intermediate revisions not shown)
Line 1: Line 1:
{{Template:EPFL2013Header}}
{{Template:EPFL2013Header}}
 +
<font size = "4"> Cell Surface Display </font> <BR>
-
Sensing <BR>
+
Working samples were sent for sequencing from their miniprep with VR primers.
-
'''Primer design''' <BR>
+
''Beginning of functional assays'' <br>
-
We desinged new primers for the three sensing construct as well as for the three effector constructs. This time we were very careful to make sure that both the foreward and reverse primer had almost the same annealing temperature and that this temperature was not too high.
+
INP_Streptavidin Dead and iGEM overnight inoculation (LB + chloramphenicol) and DH5alpha competent cells from glycerol stocks plates.
 +
<br><br>
 +
 
 +
<font size = "4"> Sensing-Effector </font> <BR>
 +
 
 +
''Primer design'' <BR>
 +
-We desinged new primers for the three sensing construct as well as for the three effector constructs. This time we were very careful to make sure that both the forward and reverse primer had almost the same annealing temperature and that this temperature was not too high.

Latest revision as of 22:01, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Working samples were sent for sequencing from their miniprep with VR primers.

Beginning of functional assays
INP_Streptavidin Dead and iGEM overnight inoculation (LB + chloramphenicol) and DH5alpha competent cells from glycerol stocks plates.

Sensing-Effector

Primer design
-We desinged new primers for the three sensing construct as well as for the three effector constructs. This time we were very careful to make sure that both the forward and reverse primer had almost the same annealing temperature and that this temperature was not too high.



Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/27_August_2013"