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Team:Goettingen/Team/Reporters
From 2013.igem.org
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<p>The activity of transcriptional regulators can be easily monitored by expressing a reporter gene downstream of a promoter, containing their binding site. Recently, in Mycobacterium smegmatis, a transcriptional repressor (DarR) was identified that is able to bind to a specific DNA sequence upon association with c-di-AMP. This led us to the idea of developing a screening system for potential drugs interfering with c-di-AMP biofunction. While many gram-positive bacteria require c-di-AMP for their growth, this molecule is not synthesized by the gram-negative bacterium E. coli. Thus, we intend to build a reporter system consisting of the iGEM biobricks in E. coli.</p> | <p>The activity of transcriptional regulators can be easily monitored by expressing a reporter gene downstream of a promoter, containing their binding site. Recently, in Mycobacterium smegmatis, a transcriptional repressor (DarR) was identified that is able to bind to a specific DNA sequence upon association with c-di-AMP. This led us to the idea of developing a screening system for potential drugs interfering with c-di-AMP biofunction. While many gram-positive bacteria require c-di-AMP for their growth, this molecule is not synthesized by the gram-negative bacterium E. coli. Thus, we intend to build a reporter system consisting of the iGEM biobricks in E. coli.</p> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/6/65/Goe-reporter-1.png" style="width: | + | <img src="https://static.igem.org/mediawiki/2013/6/65/Goe-reporter-1.png" style="width:60%" /> |
| - | <img src="https://static.igem.org/mediawiki/2013/c/c5/Goe-reporter-2.png" style="width: | + | <img src="https://static.igem.org/mediawiki/2013/c/c5/Goe-reporter-2.png" style="width:60%;" /> |
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Revision as of 19:23, 26 June 2013
