Team:HokkaidoU Japan/Shuffling Kit/Future Work

From 2013.igem.org

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<h1>Maestro E.coli Future Work</h1>
<h1>Maestro E.coli Future Work</h1>
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<h2>To make Promoter Selector better kit</h2>
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<p>To make Promoter Selector better, we are going to put pigment producing constructs between two PstI sites. After you confirm promoter, you can remove pigment producing constructs by PstI.</p>
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<div class="fig fig800">
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  <img src="https://static.igem.org/mediawiki/2013/3/3b/Maestro_HokkaidoU_2013.png">
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  <div>fig.1</div>
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</div>
<h2>Select Promoter & RBS</h2>
<h2>Select Promoter & RBS</h2>

Revision as of 05:24, 27 October 2013

Maestro E.coli

Optimization Kit

Maestro E.coli Future Work

To make Promoter Selector better kit

To make Promoter Selector better, we are going to put pigment producing constructs between two PstI sites. After you confirm promoter, you can remove pigment producing constructs by PstI.

fig.1

Select Promoter & RBS

To select proteins expressions in wider range, you can use Promoter Selector and RBS Selector at the same time! The combination number is 320 patterns!

fig.1

Besides, you can create promoter and terminator by yourself and assemble them with our RBS Selector and GGA VECTOR (K1084301) by Golden Gate Assembly!