From 2013.igem.org

• Project: Plasticity
  • At a glance
  • Waste Issue
  • M.A.P.L.E. Modules
    • M1: Resource-full Waste
    • M2: Plastic Fantastic
  • Bioplastic applications
• Human Practices
  • M.A.P.L.E
  • Industrial Implementation
  • Social Challenge
  • Communicating our Project
  • Project Evolution
• Achievements
  • Judging Criteria
  • Main Results
  • Collaborations
  • Biobricks
• In the Lab
  • Safety
  • Lab book
  • Modelling Notes
    • Introduction
    • Beta amyloid degradation
  • Data
    • Growth Assays
    • Western Blot
    • Enzyme Kinetics
    • PHB production
    • 3HB assay
    • Clearing Assays
    • Electron microscopy
  • Protocols
• Team
  • Students
  • Advisors
  • Sponsors
  • Attributions

PHB production

Nile red staining

left control cells right phaCAB transformed MG1655

O/N cutures of MG1655 transformed with either control or phaCAB plasmid were spread onto LB-agar plates with 3% glucose and Nile red staining. The staining indicates the production of P3HB.

Purification of P3HB

P(3HB) purified from phaCAB transformed MG1655 that were grown in LB with 3% glucose

P(3HB) purified from phaCAB transformed MG1655 that were grown in LB with 3% glucose

P(3HB) purified from phaCAB transformed MG1655 that were grown in M9M mixed waste.

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