Team:Marburg/Notebook:Template

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{{:Team:Marburg/Template:Header}}
 
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{{:Team:Marburg/Template:ContentTitle}}
 
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Notebook
 
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{{:Team:Marburg/Template:ContentStart}}
 
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<html>
 
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<!-- Beispiel Allgemein -->
 
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<div class="notebooky-entry">
 
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<h2 class="title">
 
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<a name="11-11-2012">11.11.2011</a>
 
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</h2>
 
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<fieldset class="experiment general">
 
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<legend><a name="title">Überschrift</a></legend>
 
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<div class="investigator">
 
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<span class="inv">Investigator:</span>
 
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<span class="inv-names">X,Y</span>
 
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</div>
 
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<div class="aim">
 
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<span class="aim">Aim:</span>
 
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<span class="aim-desc">We attempt to get results.</span>
 
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</div>
 
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<div class="exp-content">
 
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<p>Wichtig ist, dass immer die verwendete Materialien (Plasmide, genomische DNA [aus welchen Stamm],
 
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Primer) genau benannt und/oder ggf. irgendwo nummeriert werden. Es sollte alles nachvollziehbar sein,
 
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wann ihr mit welchen Proben was gemacht habt.</p>
 
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<p></p>
 
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<p>Auf den folgenden Seiten sind Beispiele, wie wir uns das Notebook vom Aufbau und Detailgrad vorstellen.</p>
 
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</div>
 
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</fieldset>
 
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</div>
 
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-
 
-
 
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<!-- Beispiel Ligation, Verdau -->
 
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<div class="notebooky-entry">
 
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<h2 class="title">
 
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<a name="17-04-2013">17.04.2013</a>
 
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</h2>
 
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<fieldset class="experiment ligation">
 
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    <legend><a name="lig">Ligation</a></legend>
 
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    <div class="investigator">
 
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<span class="inv">Investigator:</span>
 
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<span class="inv-names">Franzi, Lucas</span>
 
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</div>
 
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    <div class="aim">
 
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<span class="aim">Aim:</span>
 
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<span class="aim-desc">Assemble the Bricks 1, 3, 4, 5, 6, 7 and 8 into the vector pSB1C3.</span>
 
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</div>
 
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<div class="exp-content">
 
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<ul class="lig">
 
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<li>5 µl vector DNA (pSB1C3)</li>
 
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<li>20 µl insert DNA (1, 3, 4, 5, 6, 7 or 8)</li>
 
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<li>3 µl 10x T4 DNA ligase buffer</li>
 
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<li>2 µl T4 DNA ligase</li>
 
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</ul>
 
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<p>The samples were incubated for 14 h at 18° C.</p>
 
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</div>
 
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</fieldset>
 
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</div>
 
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<!-- Beispiel Verdau -->
 
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<fieldset class="experiment digest">
 
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    <legend><a name="dig">Digest</a></legend>
 
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    <div class="investigator">
 
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<span class="inv">Investigator:</span>
 
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<span class="inv-names">Christian, Patrick</span>
 
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</div>
 
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    <div class="aim">
 
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<span class="aim">Aim:</span>
 
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<span class="aim-desc">Digest of pSB1C3-J04450 with <i>Mlu</i>I and <i>Hind</i>III.</span>
 
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</div>
 
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<div class="exp-content">
 
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<ul class="digest">
 
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<li>4 µl DNA (pSB1C3-J04450)</li>
 
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<li>1 µl MluI</li>
 
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<li>1 µl HindIII</li>
 
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<li>1,5 µl 10x red buffer</li>
 
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<li>1,5 µl 10x orange g loading buffer</li>
 
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<li>6 µl H2O</li>
 
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</ul>
 
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<p>The samples were incubated for 4 h at 37° C.</p>
 
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<table class="gel digest">
 
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<colgroup>
 
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<col width="50%" />
 
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<col width="50%" />
 
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</colgroup>
 
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<thead>
 
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<tr>
 
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<th colspan="2" class="title">Gel electrophoresis</th>
 
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</tr>
 
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</thead>
 
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<tbody>
 
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<tr>
 
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<td>
 
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<img src="beispiel.png" width="50%" alt="gel-electrophoresis-image" />
 
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</td>
 
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<td>
 
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<p>
 
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<span class="gel-elc">Gel substances</span>
 
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<ul class="gel-sub">
 
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<li>1% Agarose gel</li>
 
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<li>10 µl RedSafe in 50 ml gel</li>
 
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<li>x µl Hyper Ladder</li>
 
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</ul>
 
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</p>
 
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<p>
 
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<span class="exp">Expactations</span>
 
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<ul class="exp">
 
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<li>Lane 1: 5300 kbp</li>
 
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<li>Lane 2: 3300 kbp</li>
 
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<li>Lane 3: 1300 kbp</li>
 
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</ul>
 
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</p>
 
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<p>We don’t receive all expected fragments. The expected fragment in lane 1 is missing.</p>
 
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</td>
 
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</tr>
 
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<tr>
 
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<td colspan="2" class="gel-fazit">
 
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<p>We don’t receive all expected fragments. The expected fragment in lane 1 is missing.</p>
 
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</td>
 
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</tr>
 
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</tbody>
 
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</table>
 
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</div>
 
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</fieldset>
 
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<!-- Beispiel Transformation, PCR -->
 
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<div class="notebooky-entry">
 
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<h2 class="title">
 
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<a name="18-04-2012">18.04.2012</a>
 
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</h2>
 
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<fieldset class="experiment transformation">
 
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    <legend>Transformation</legend>
 
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    <div class="investigator">
 
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<span class="inv">Investigator:</span>
 
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<span class="inv-names">Franzi, Christian</span>
 
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</div>
 
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    <div class="aim">
 
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<span class="aim">Aim:</span>
 
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<span class="aim-desc">Transformation of the plasmid DNA pB201-40JO in <i>E. coli</i> for amplification.</span>
 
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</div>
 
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<div class="exp-content">
 
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<p>The chemo competent <i>E. coli</i> DH5a cells were transformed with pB201-40JO DNA and plated on dYT-Amp-plates. <br />The plates were incubated over night at 37° C .</p>
 
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</div>
 
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</fieldset>
 
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<!-- PCR -->
 
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<fieldset class="experiment pcr">
 
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    <legend><a name="pcr">PCR</a></legend>
 
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    <div class="investigator">
 
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<span class="inv">Investigator:</span>
 
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<span class="inv-names">Patrick, Lucas</span>
 
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</div>
 
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    <div class="aim">
 
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<span class="aim">Aim:</span>
 
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<span class="aim-desc">Receive <i>Pvu</i>II point-mutation on colonies 3 and 5 of E. coli pB201-40JO.</span>
 
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</div>
 
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<div class="exp-content">
 
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<table class="pcr">
 
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<colgroup>
 
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<col width="15%" />
 
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<col width="30%" />
 
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<col width="5%" />
 
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<col width="20%" />
 
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<col width="20%" />
 
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<col width="10%" />
 
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</colgroup>
 
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<thead>
 
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<th>Volume</th>
 
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<th>Reagent</th>
 
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<th>&nbsp;</th>
 
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<th>Temp (°C)</th>
 
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<th colspan="2">Time</th>
 
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</thead>
 
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<tr>
 
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<td>10 µl</td>
 
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<td>5x Buffer</td>
 
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<td>&nbsp;</td>
 
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<td>95</td>
 
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<td colspan="2">3 min</td>
 
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</tr>
 
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<tr>
 
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<td>1,5 µl</td>
 
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<td>Primer fwd 13</td>
 
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<td>&nbsp;</td>
 
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<td>95</td>
 
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<td colspan="2">3 sec</td>
 
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</tr>
 
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<tr>
 
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<td>1,5 µl</td>
 
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<td>Primer rev 14</td>
 
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<td>&nbsp;</td>
 
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<td>58</td>
 
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<td>30 sec</td>
 
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<td rowspan="3">x17</td>
 
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</tr>
 
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<tr>
 
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<td>1 µl</td>
 
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<td>Template</td>
 
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<td>&nbsp;</td>
 
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<td>72</td>
 
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<td>1 min</td>
 
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</tr>
 
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<tr>
 
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<td>36 µl</td>
 
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<td>H2O</td>
 
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<td>&nbsp;</td>
 
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<td>7 min</td>
 
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<td>3 min</td>
 
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</tr>
 
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<tr>
 
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<td>1 µl Phusion</td>
 
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<td>Phusion Polymerase</td>
 
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<td>&nbsp;</td>
 
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<td>4</td>
 
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<td colspan="2"><span class="hold">Hold</span></td>
 
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</tr>
 
-
</table>
 
-
<br />
 
-
<!-- Das Gel-Bild fast gleich wie beim Verdau -->
 
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<table class="gel pcr">
 
-
<colgroup>
 
-
<col width="50%" />
 
-
<col width="50%" />
 
-
</colgroup>
 
-
<thead>
 
-
<tr>
 
-
<th colspan="2" class="title">Gel electrophoresis</th>
 
-
</tr>
 
-
</thead>
 
-
<tbody>
 
-
<tr>
 
-
<td>
 
-
<img src="beispiel.png" width="50%" alt="gel-electrophoresis-image" />
 
-
</td>
 
-
<td>
 
-
<p>
 
-
<span class="gel-elc">Gel substances</span>
 
-
<ul class="gel-sub">
 
-
<li>1% Agarose gel</li>
 
-
<li>10 µl RedSafe in 50 ml gel</li>
 
-
<li>x µl Hyper Ladder</li>
 
-
</ul>
 
-
</p>
 
-
<p>
 
-
<span class="exp">Expactations</span>
 
-
<ul class="exp">
 
-
<li>Lane 1: 5300 kbp</li>
 
-
<li>Lane 2: 3300 kbp</li>
 
-
<li>Lane 3: 1300 kbp</li>
 
-
</ul>
 
-
</p>
 
-
<p>We don’t receive all expected fragments. The expected fragment in lane 1 is missing.</p>
 
-
</td>
 
-
</tr>
 
-
</tbody>
 
-
</table>
 
-
</div>
 
-
</fieldset>
 
-
</div>
 
-
 
-
</html>
 
-
 
-
{{:Team:Marburg/Template:ContentEnd}}
 
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{{:Team:Marburg/Template:Footer}}
 

Latest revision as of 09:09, 4 October 2013