Team:NTNU-Trondheim/3 July 2013
From 2013.igem.org
Wednesday 03.07.2013
The small-scale vesicle preparation continued with completion of step 2-7 in the vesicle isolation protocol. There where made three samples in step 2; E.coli ER2566 in LB, E.coli BW27784 in LB with ampenicillin (100 µg/mL) (ara-) and E.coli BW27784 in LB with ampenicillin (100 µg/mL) with the addition of arabinose (0.5 %) after 1 hour of incubtion (ara+).
Optical denisty (OD) at 600 nm was mesured as indicated in the table below.
Sample | OD600 | Estimated CFU |
ER2566 | 0.9805 | |
ara- | 0.1263 | |
ara+ | 0.0471 |
We isolated the DNA from the transformed cells (with RBS, pBAD and pTet) by using the miniprep protocol and measured the concentration by [http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf NanoDrop ND-1000 Spectrophotometer].
Sample | Concentration (ng/µl) |
RBS | 4,91 |
pTet1 | 8,99 |
pTet2 | 4,39 |
pBAD1 | 3,54 |
pBAD2 | 9,24 |