From 2013.igem.org
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| - | <html>
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| - | <div class ="tbnote">
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| - | <h2>Phage Sensor</h2>
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| - | <a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Phage_Sensor" target="_blank" class="tbnotelogo PSlogo"> ASDF </a>
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| - | <div style="clear: both;"></div>
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| - | <h3>Friday 6<sup>th</sup> September</h3>
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| - | <p><b><em>
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| - | <!-- === Modify from here === -->
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| - | Place your twit here
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| - | <!-- === To here === -->
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| - | </br></em></b></p>
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| - | <!-- === Modify from here === -->
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| - | Digestions: linearized pSB1C3, linearized pSB1A3, SPCR2, SPCR3, SPCR11<br>
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| - | <br>
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| - | <table border="1">
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| - | <tr>
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| - | <td><b>Reagent</b></td>
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| - | <td><b>Volume</b></td>
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| - | </tr>
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| - | <tr>
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| - | <td>Purified PCR Product</td>
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| - | <td>16 µl</td>
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| - | </tr>
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| - | <tr>
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| - | <td>H2OH2O</td>
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| - | <td>0 µl</td>
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| - | </tr>
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| - | <tr>
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| - | <td>10x FastDigest Buffer</td>
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| - | <td>2 µl</td>
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| - | </tr>
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| - | <tr>
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| - | <td>FastDigest Enzyme 1</td>
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| - | <td>1 µl</td>
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| - | </tr>
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| - | <tr>
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| - | <td>cFastDigest Enzyme 2</td>
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| - | <td>1 µl</td>
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| - | </tr>
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| - | <tr>
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| - | <td><b>Total Volume</b></td>
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| - | <td>4 µl</td>
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| - | </tr>
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| - | </table><br>
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| - | <br>
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| - | SPCR5, pSB1C3: XbaI/SpeI<br>
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| - | SPCR11, pSB1A3, pSB1C3: EcoRI/PstI<br>
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| - | SPCR13, pSB1A3, pSB1C3: EcoRI/PstI<br>
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| - | SPCR2, pSB1A3, pSB1C3: EcoRI/PstI<br>
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| - | <br>
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| - | 1h at 37° shaking<br>
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| | | | |
| - | <!-- === To here === -->
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| - | </div>
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| - | </html>
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Latest revision as of 16:41, 25 September 2013
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