Team:Paris Bettencourt/Notebook/Trojan Horse/Saturday 24th August.html

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<h2>Trojan Horse</h2>
 
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<a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Trojan_Horse" target="_blank" class="tbnotelogo THlogo"> ASDF </a>
 
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<h3>24<sup>th</sup> August</h3>
 
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    Results: failure: as much GFP+ colonies growing on Kan than on LB
 
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    <em>PCR from mini Gene sRNA anti Cm</em>
 
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    35 H2O
 
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    10 Buffer 5x
 
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    1 dNTP
 
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    2 FW iT009
 
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    2 RV iT0010
 
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    0.2 minigene-plasmid amp from IDT
 
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    0.5 phusion
 
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    PCR Phusion Gradient around 53 &#176;C
 
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    Time : 30 secondes
 
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Latest revision as of 17:59, 3 October 2013