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Team:TU-Delft/ProtocolList
From 2013.igem.org
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<li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_13#protocol_13" style="text-decoration: none""><font color="#0080FF" size="3">AIP Sensing Protocol</font></a> </li> | <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_13#protocol_13" style="text-decoration: none""><font color="#0080FF" size="3">AIP Sensing Protocol</font></a> </li> | ||
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| + | <li> <a href="https://static.igem.org/mediawiki/2013/6/6b/Gene_synthesis.pdf" style="text-decoration: none""><font color="#0080FF" size="3">Gene Design</font></a> </li> | ||
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| + | <li> <a href="https://static.igem.org/mediawiki/2013/3/3f/Primers.pdf" style="text-decoration: none""><font color="#0080FF" size="3">Primer Design</font></a> </li> | ||
</ul> | </ul> | ||
Revision as of 18:07, 4 October 2013
Protocols
Our project deals with E.coli cells which sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and starts producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Different protocols used during the project are described below.
- Transforming Parts from Distribution kit
- Growing the Single Colonies from the Agar Plates
- Making glycerol stocks
- Plasmid Purification Protocol
- Restriction digestion
- Ligation
- Gel Extraction Procedure
- PCR Purification
- Tricine Gels
- General Peptide Production
- SUMO cleavage
- Lysis Protocol
- AIP Sensing Protocol
- Gene Design
- Primer Design
