Team:Tianjin/Project/Experiment/TetA

From 2013.igem.org

(Difference between revisions)
(Library Construction)
(Blanked the page)
 
(8 intermediate revisions not shown)
Line 1: Line 1:
-
<html>
 
-
</div>
 
-
</div>
 
-
</div>
 
-
 
-
<style type="text/css">
 
-
*{margin:0;padding:0;font-family:"微软雅黑","Arial";}
 
-
 
-
body{
 
-
width: 960px;
 
-
height: auto;
 
-
margin: 0 auto;
 
-
font-size:12px;
 
-
font-family:Arial, Helvetica, sans-serif;
 
-
background-color:#fff;
 
-
}
 
-
 
-
h1,h2,h3,h4,h5,h6{
 
-
border:none;}
 
-
 
-
p {margin:0.5em 0 !important;
 
-
text-decoration:none;
 
-
font-family:Arial, Helvetica, sans-serif;
 
-
font-size:12px;
 
-
}
 
-
.content-section{
 
-
width:960px;
 
-
height:10px;
 
-
margin-top:0px;
 
-
}
 
-
.logo-section{
 
-
height:220px;
 
-
width:960px;
 
-
position:relative;
 
-
float:left;
 
-
margin-top:5px;
 
-
background:url(https://static.igem.org/mediawiki/igem.org/1/16/Tju-Project-banner.jpg) no-repeat;
 
-
}
 
-
.note{
 
-
width:960px;
 
-
height:200px;
 
-
float:left;
 
-
margin-top:10px;
 
-
position:relative;
 
-
background-color:#fff;
 
-
padding:0;
 
-
}
 
-
.note1{
 
-
width:960px;
 
-
min-height:200px;
 
-
float:left;
 
-
background-color:#fff;
 
-
margin-top:0px;
 
-
}
 
-
.note2{
 
-
width:880px;
 
-
height:auto;
 
-
float:left;
 
-
position:relative;
 
-
margin-left:20px;
 
-
padding:30px 10px 10px 30px;
 
-
}
 
-
#footer{
 
-
margin:0 auto;
 
-
width:960px;
 
-
min-height:73px;
 
-
text-align:center;
 
-
color:#666;
 
-
float:left;
 
-
}
 
-
#goTopBtn {POSITION: fixed;TEXT-ALIGN: center;LINE-HEIGHT: 30px;WIDTH: 100px;BOTTOM: 35px;HEIGHT: 100px;FONT-SIZE: 12px;RIGHT: 30px;}
 
-
 
-
 
-
<!--hidden section-->
 
-
.hidden {display:none;}
 
-
#p-logo,#footer-box {display:none;}
 
-
#top-section {border:none;height:15px;}
 
-
.firstHeading {display:none;}
 
-
#bodyContent{display:none;}
 
-
#menubar a{text-decoration:none;color:#CCC;list-style: none;}
 
-
#menubar a:hover{text-decoration:none;color:#FFF;list-style: none;}
 
-
#search-controls{display:none;}
 
-
.printfooter{display:none;}
 
-
#catlinks{display:none;}
 
-
#content{background:none;padding:0px;margin-top:0px;border:none;}
 
-
#toc{display:none;}
 
-
</style>
 
-
 
-
<!--content section-->
 
-
 
-
<style type="text/css">
 
-
.sddm{z-index: 30;width: 960px;height:65px;position:relative;float:left;background-color:#FFF;position:raletive;margin-left:0px;}
 
-
.sddm ul{margin-left:0px;}
 
-
.sddm li{margin: 0;list-style: none;float: left;font: bold 14px arial;height:25px;background-color:#FFF;}
 
-
.sddm li a{display: block;margin:0;padding: 15px 0px 15px 0px;width: 96px;background: #FFF;color: #000;text-align: center;text-decoration: none;border-top:#ececec 5px solid;}
 
-
.sddm li a:hover{background:#FCFCFC;color:#0babe7;border-top:#0babe7 5px solid;}
 
-
.sddm div{position: absolute;width:96px;z-index:999;visibility: hidden;margin: 0;padding: 0;}
 
-
.sddm div a{position: relative;display: block;margin: 0;padding: 5px 5px;width: auto;white-space: nowrap;text-align:center;text-decoration: none;color: #000;font: 12px arial}
 
-
.sddm div a:hover{background:#FAFAFA;color: #0babe7;}
 
-
</style>
 
-
 
-
 
-
 
-
<div class="cont1">
 
-
<ul class="sddm">
 
-
 
-
<style type="text/css">
 
-
.A-C{font-size:12px !important;padding-top:0 !important;text-align:center;line-height:100%}
 
-
.HP{font-size:14px !important;padding-top:0 !important;text-align:center;}
 
-
</style>
 
-
 
-
    <li><a href=" https://2013.igem.org/Team:Tianjin" style="padding:20px 0px 15px 0px;height:30px;">Home</a>
 
-
</li>
 
-
    <li><a href=" https://2013.igem.org/Team:Tianjin/Project" style="padding:20px 0px 15px 0px;height:30px;">Project</a>
 
-
</li>
 
-
    <li><a href=" https://2013.igem.org/Team:Tianjin/Safety "  style="padding:20px 0px 15px 0px;height:30px;">Safety</a>
 
-
</li>
 
-
    <li><a href=" https://2013.igem.org/Team:Tianjin/Modeling"  style="padding:20px 0px 15px 0px;height:30px;">Modeling</a>
 
-
</li>
 
-
    <li><a href=" https://2013.igem.org/Team:Tianjin/Protocol"  style="padding:20px 0px 15px 0px;height:30px;">Protocol</a>
 
-
</li>
 
-
    <li class="HP"><a href="https://2013.igem.org/Team:Tianjin/Human Practice" style="padding:14px 0px 13px 0px;">Human Practice</a>
 
-
        </li>
 
-
    <li><a href=" https://2013.igem.org/Team:Tianjin/Data"  style="padding:20px 0px 15px 0px;height:30px;">Data</a>
 
-
</li>
 
-
    <li><a href=" https://2013.igem.org/Team:Tianjin/Notebook"  style="padding:20px 0px 15px 0px;height:30px;">Notebook</a>
 
-
</li>
 
-
    <li><a href=" https://2013.igem.org/Team:Tianjin/Team"  style="padding:20px 0px 15px 0px;height:30px;">Team</a>
 
-
</li>
 
-
    <li class="A-C"><a href="https://2013.igem.org/Team:Tianjin/Attributions"  style="padding:7px 0px 7px 0px;">Attributions<br/>&amp;<br/>Contributions</a>
 
-
        </li>
 
-
</ul>
 
-
</div>
 
-
 
-
 
-
 
-
<div class="content-section">
 
-
</div>
 
-
 
-
<div class="logo-section">
 
-
</div>
 
-
 
-
 
-
 
-
<div class="note1">
 
-
<style type="text/css">
 
-
.box{ width:960px; margin:0 auto; overflow:hidden;}
 
-
.main{ width:720px; height:auto; float:right;position:relative;padding:10px 10px 10px 10px;}
 
-
.fixed{ width:220px; height:400px;text-align:center;float:left;word-spacing:0.1em;top:10px;margin-top:10px;}
 
-
.main img{border:hidden;margin-bottom:5px;}
 
-
.main div,li,p{font-family:Arial;line-height:150%;word-spacing:0.1em;}
 
-
 
-
 
-
.box p{color:#000;font-family:Arial, Helvetica, sans-serif;font-size:14px;line-height:150%;text-align:left; clear:both;}
 
-
.box h1{text-decoration:none;font-weight:normal;color:#0babe7;font-family:Arial;}
 
-
.img1{margin:0 150px 15px 150px;padding:5px 5px 5px 5px;background-color:#fafafa;border:thin solid #999; vertical-align:middle;width:400px;}
 
-
.img1 a{target="_blank";}
 
-
 
-
 
-
 
-
</style>
 
-
 
-
<script type="text/javascript">
 
-
$(document).ready(function(e) {
 
-
t = $('.fixed').offset().top;
 
-
mh = $('.main').height();
 
-
fh = $('.fixed').height();
 
-
$(window).scroll(function(e){
 
-
s = $(document).scrollTop();
 
-
if(s > t - 100){
 
-
$('.fixed').css('position','fixed');
 
-
if(s + fh - 6000 > mh){
 
-
$('.fixed').css('top',mh-s-fh+'px');
 
-
}
 
-
}else{
 
-
$('.fixed').css('position','');
 
-
}
 
-
})
 
-
});
 
-
</script>
 
-
 
-
 
-
 
-
<div class="box">
 
-
 
-
<div class="sub">
 
-
<div class="fixed">
 
-
<style type="text/css">
 
-
li{margin-bottom:0px;}
 
-
.cont ul,li{list-style: none;} 
 
-
.cont ul {padding: 0; margin: 0;text-align:center;} 
 
-
.cont .hmain{background-color:#0babe7 ;width: 220px;font-size:16px;float: left;border-top:#fff thin solid;border-bottom:#fff thin solid;} 
 
-
 
 
-
.cont a {text-decoration: none; /* padding-left: 10px;*/  display: block;  display: inline-block; width: 220px;padding-top: 7px;padding-bottom: 7px;} 
 
-
.cont .hmain a{color:#fff;  }
 
-
.cont .hmain a:hover{color:#0babe7;background:#fff;border-top:#0babe7 thin solid;border-bottom:#0babe7 thin solid;  } 
 
-
.cont .hmain ul {display: none;} 
 
-
.cont .hmain li a{font-size:14px;color:#fff;background-color:#8dc7e9;line-height:150%;border:#fff thin solid ;}
 
-
.cont .hmain li a:hover{color:#8dc7e9;background:#fff; line-height:150%;border:#8dc7e9 thin solid ;} 
 
-
</style>
 
-
 
-
<script type="text/javascript">
 
-
$(document).ready(function(){ 
 
-
    $(".hmain").hover(function(){ 
 
-
        $(this).children("ul").slideDown(); 
 
-
        changeIcon($(this).children("a")); 
 
-
    },function(){ 
 
-
        $(this).children("ul").slideUp(); 
 
-
        changeIcon($(this).children("a")); 
 
-
    }); 
 
-
});
 
-
</script>
 
-
<div class="cont">
 
-
        <ul>
 
-
            <li class="hmain" style="margin-top:20px;">
 
-
 
-
            <li class="hmain">
 
-
                <a href="https://2013.igem.org/Team:Tianjin/Project">Background</a>
 
-
            </li>
 
-
            <li class="hmain">
 
-
                <a href="https://2013.igem.org/Team:Tianjin/Project/Design">Design</a>
 
-
            </li>
 
-
            <li class="hmain">
 
-
                <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment">Experiment&Result</a>
 
-
                <ul>
 
-
                    <li>
 
-
                        <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment">System construction and optimization</a>
 
-
                    </li>
 
-
                    <li>
 
-
                        <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment/Endo-Induce">Endogenous Alkanes Induce</a>
 
-
                    </li>
 
-
                    <li>
 
-
                        <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment/Exo-Induce">Exogenous Alkanes Induce</a>
 
-
                    </li>
 
-
                    <li>
 
-
                        <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment/TetA">Endogenous Induce&Resistance Selection</a>
 
-
                    </li>
 
-
                </ul>
 
-
            </li>
 
-
 
-
            <li>
 
-
                <a href="https://2013.igem.org/Main_Page" title="Main Page"><img src="https://static.igem.org/mediawiki/2013/7/75/IGEM-logo-blue.png" width="200px" height="200px" border="none" style="margin-top:80px;"/></a>
 
-
            </li>
 
-
        </ul>
 
-
</div>
 
-
</div>
 
-
</div>
 
-
 
-
 
-
<div class="main">
 
-
 
-
<center><span style="font-family:Arial;font-size:46px;color:#000;"> Experiment</span></center>
 
-
</html>
 
-
 
-
=Endogenous Induce&Resistance Selection=
 
-
 
-
 
-
<html>
 
-
<hr/>
 
-
<br/>
 
-
 
-
<p>Here, we want to test the resistance against tetracycline of Alk-Sensor induced by endogenous alkanes.</p>
 
-
<p>We put the alkane producing module into cells to test the function of TetA:</p>
 
-
<br/>
 
-
<div style="text-align:center;vertical-align:middle;"><a href="https://static.igem.org/mediawiki/2013/d/db/Tju-p-1.jpg" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/d/db/Tju-p-1.jpg" width="600px" /></a></div>
 
-
 
-
<p><b>Construct:</b></p>
 
-
<p>Left: No.60: TetA downstream of Palkm and AlkR downstream of J23103, are cloned into vector PSB1C3 and there’s a alkane producing vector PSB3K3.</p>
 
-
<p>Right: No.61: We construct a blank vector PSB3K3 without NPDC and AAR as blank control, and the other vector PSB1C3 is the same as No.60.</p>
 
-
<br/>
 
-
<p><b>Characterization: </b></p>
 
-
<p>Strains of No.60 are cultured in LB medium for about 12 hours. Add 17μg/mL Kan, 12μg/mL Cm and 12μg/mL Tc respectively. After that, inoculate into 3 ml LB medium for an overnight cultures at 37 ℃ with 220rpm shaking.</p>
 
-
 
-
<br/>
 
-
<div style="text-align:center;vertical-align:middle;"><a href="https://static.igem.org/mediawiki/2013/1/1e/DSC00002-1.jpg" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/1/1e/DSC00002-1.jpg" width="500px" /></a></div>
 
-
 
-
 
-
<p><b>Result:</b> </p>
 
-
<p>Alkane producing E.coli can grow in tube but blank vector E.coli cannot grow .</p>
 
-
<p><b>Conclusion:</b></p>
 
-
<p>Synthesized alkanes induce the expression of alkR, which enables E.coli to survive under the pressure of Tetracycline.</p>
 
-
<br/>
 
-
<p>Thus, we can get a 3×3 matrix, with promoter strength from weak, medium to strong, and plasmid copy number from small, medium to large.</p>
 
-
 
-
<br/>
 
-
<div style="text-align:center;vertical-align:middle;"><a href="https://static.igem.org/mediawiki/2013/5/57/Tju-p-2.jpg" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/5/57/Tju-p-2.jpg" width="720px" /></a></div>
 
-
<br/>
 
-
 
-
 
-
 
-
 
-
</div>
 
-
 
-
</div>
 
-
 
-
</div>
 
-
 
-
 
-
 
-
 
-
<div class="note2">
 
-
 
-
</div>
 
-
 
-
<!--
 
-
<div class="note">
 
-
 
-
</div>
 
-
<div class="note">
 
-
 
-
</div>
 
-
-->
 
-
 
-
</div>
 
-
 
-
 
-
 
-
<div id="footer">
 
-
<p><br />
 
-
<br />
 
-
E-mail:austinamens@gmail.com | Address:Building No.20, No.92 Weijin Road, Tianjin, China | Zip-code:300072
 
-
<br />Copyright 2013 © Tianjin University iGEM Team
 
-
</p>
 
-
</div>
 
-
 
-
<!--top button section-->
 
-
<div style="display: block" id="goTopBtn">
 
-
<a href="#top" title="Top"><img border=0 src="https://static.igem.org/mediawiki/2013/6/61/Top.jpg"></a>
 
-
</div>
 
-
 
-
</html>
 

Latest revision as of 02:27, 29 October 2013