April

18

Our first official gathering of Tokyo-NoKoGen 2013! Okay, let’s deicde on what we will do for the project this year.

25

Before we start, we looked up on what projects have been in presented in the past iGEM competitions. Each one of us picked a team of our interest, and made a presentation to the team.

May

1
To understand how iGEM work, we made a seminar to understand the basics, for example how to use restriction enzymes, and how to use the BioBrick parts.

16
Since we are going to present at Tokyo University very very soon, we need to practice and revise our poster presentation...Are we all ready?

19
School festival at Tokyo University! We made a poster presentation to many high school students and parents on our last year's project. It was very interesting to explain about genetic engineering of E. coli to those who are not familiar with them! We also got to know other team members of iGEM Japan, they were people who were fun to be with.

25
Homework for the next iGEM meeting - each person must look up on a recent paper and make a presentation to the team.

June

6
Brainstorming...production of biodiesel? Make fertilisers using E. coli?

20
Another brainstorming...shall we make an extended version of lux luminescence? Anyone with good idea? Since we are running out of time, we wil make it a compulsory for each person to come up with at least 3 ideas!

July

4
Everyone came up with many interesting ideas over the past two weeks. Hmmm....which one shall we challenge? Not so much time left.

11
Brainstorming...Ooo, HHR, RNA oscillator...?

August

14
Yay, we got BBa_K1053000, our first ribozyme!

25
A team interaction with iGEM Japan team members at Tokyo metropolitan university!

28
We dicided on wiki, poster and oral presentation members

30
We got BBa_K1053003 and BBa_K1053004

September

3
Made TOP10 competent cells

9
Construction of pSB3C5-PBAD-HHR-GRP-DT and evaluation. Hopefully it works!

10
Get ready for BioBrick submition! Woohoo

13
RE-evaluation of pSB3C5-PBAD-HHR-GRP-DT and evaluation. Hopefully it works this time.

16
We got three HHRs composing RNA oscillator and HHR fused with RNA scaffold. But oh no...they have extra XbaI :(

20
Evaluation of pSB3C5-PBad-tr(12)-HHR-GFP-DT by taking a time course. When is the optimal time to see the difference?

26
Evaluation of pSB1A3-PBAD-tr(42)-HHR-taR(12)-DT-Pconst(H)-taR(42)-DT

27
Wiki FREEZE

October

5
Asian Regional Jamboree

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