Team:Tuebingen/Notebook/Journal

From 2013.igem.org

(Difference between revisions)
 
(23 intermediate revisions not shown)
Line 10: Line 10:
   min-height: 5em;
   min-height: 5em;
   min-width: 5em;
   min-width: 5em;
-
  margin-bottom: 1em;
 
}
}
Line 18: Line 17:
   width: 100px;
   width: 100px;
   position: relative;
   position: relative;
 +
  padding-right: 1em;
}
}
Line 30: Line 30:
}
}
 +
#actualContent p {
 +
  line-height: 1.5em;
 +
  margin-bottom: 16px;
 +
}
</style>
</style>
</head>
</head>
Line 36: Line 40:
<div id="actualHeadingOne">
<div id="actualHeadingOne">
-
<span style="font-size: 50px">W</span>eekly <span style="font-size: 50px">J</span>ournal
+
Overview: Journal
</div>
</div>
Line 44: Line 48:
<div class="journalWrapper">
<div class="journalWrapper">
   <div class="journalDiv">
   <div class="journalDiv">
-
     <img src="https://static.igem.org/mediawiki/2013/5/54/TueSmallReceptor.PNG">
+
     <a href="#"><img src="https://static.igem.org/mediawiki/2013/5/54/TueSmallReceptor.PNG"></a>
-
     p><b>Receptor</b>: for simplification we denote the membranous progestin receptors of <i>Danio rerio</i> and <i>Xenopus laevis</i> as "Receptor". Since the Receptor serves as a sensor for progestins it is a is a very crucial element in our measurement system.</p>
+
     <p><b>Receptor</b>: For simplification we denote the membranous progestin receptors (mPR) of <i>Danio rerio</i> and <i>Xenopus laevis</i> as "Receptor". One important property of the progestin receptor is its abilitiy to inhibit the FET3 gene promoter when progestin binds to the receptor. Since the Receptor serves as a sensor for progestins it is a very crucial element in our measurement system.</p>
   </div>
   </div>
Line 51: Line 55:
   <div class="journalDiv">
   <div class="journalDiv">
-
     <img src="https://static.igem.org/mediawiki/2013/9/9e/TueSmallInverter.PNG">
+
     <a href="#"><img src="https://static.igem.org/mediawiki/2013/9/9e/TueSmallInverter.PNG"></a>
-
     <p>Item</p>
+
     <p><b>Inverter</b>: The progestin receptors inhibit the promoter of the FET3 gene (Pfet3) when progestin is present in the cell's medium. In order to receive a positive feedback when progestin is detected we need to invert the receptor signal. Therefore we have combined Pfet3 with a repressor that represses the promotor of our reporter.  </p>
   </div>
   </div>
    
    
Line 58: Line 62:
   <div class="journalDiv">
   <div class="journalDiv">
-
     <img src="https://static.igem.org/mediawiki/2013/8/8f/TueSmallReporter.PNG">
+
     <a href="#"><img src="https://static.igem.org/mediawiki/2013/8/8f/TueSmallReporter.PNG"></a>
-
     <p>Item</p>
+
     <p><b>Reporter</b>: Our aim is the construction of an easy-to-use measurement system. Therefore we decided to use a reporter that delivers optical feedback which then can be quantified with an inexpensive photometer. Thus luciferase (LUC) serves as a reporter for our measurement system.</p>
   </div>
   </div>
</div>
</div>
 +
 +
<p>In this weekly-journal you can <a href="/Team:Tuebingen/Notebook/Journal/Weekly">follow our overall progress</a>!</p>
</div>
</div>

Latest revision as of 02:02, 5 October 2013

Return to iGEM Main Page.

Overview: Journal

Receptor: For simplification we denote the membranous progestin receptors (mPR) of Danio rerio and Xenopus laevis as "Receptor". One important property of the progestin receptor is its abilitiy to inhibit the FET3 gene promoter when progestin binds to the receptor. Since the Receptor serves as a sensor for progestins it is a very crucial element in our measurement system.

Inverter: The progestin receptors inhibit the promoter of the FET3 gene (Pfet3) when progestin is present in the cell's medium. In order to receive a positive feedback when progestin is detected we need to invert the receptor signal. Therefore we have combined Pfet3 with a repressor that represses the promotor of our reporter.

Reporter: Our aim is the construction of an easy-to-use measurement system. Therefore we decided to use a reporter that delivers optical feedback which then can be quantified with an inexpensive photometer. Thus luciferase (LUC) serves as a reporter for our measurement system.

In this weekly-journal you can follow our overall progress!