Team:Tuebingen/Notebook/Journal

From 2013.igem.org

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<span style="font-size: 50px">W</span>eekly <span style="font-size: 50px">J</span>ournal
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Overview: Journal
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     <p><b>Receptor</b>: For simplification we denote the membranous progestin receptors (mPR) of <i>Danio rerio</i> and <i>Xenopus laevis</i> as "Receptor". One important property of the progestin receptor is its abilitiy to inhibit the fet3 promotor when progestin in absent. Since the Receptor serves as a sensor for progestins it is a is a very crucial element in our measurement system.</p>
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     <p><b>Receptor</b>: For simplification we denote the membranous progestin receptors (mPR) of <i>Danio rerio</i> and <i>Xenopus laevis</i> as "Receptor". One important property of the progestin receptor is its abilitiy to inhibit the FET3 gene promoter when progestin binds to the receptor. Since the Receptor serves as a sensor for progestins it is a very crucial element in our measurement system.</p>
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     <p><b>Inverter</b>: Since our progestin receptors inhibit the promotor fet3 (Pfet3) while progestin is absent in the cell's medium and allows transcription of the downstream genes of Pfet3 once progestin binds to the receptor ...</p>
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     <p><b>Inverter</b>: The progestin receptors inhibit the promoter of the FET3 gene (Pfet3) when progestin is present in the cell's medium. In order to receive a positive feedback when progestin is detected we need to invert the receptor signal. Therefore we have combined Pfet3 with a repressor that represses the promotor of our reporter. </p>
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     <p><b>Reporter</b>: Our aim is the construction of an easy to use measuring system. Therefore we decided to use reporters that deliver optical feedback which then can be quantified with an inexpensive photometer. Thus lacZ and luciferse serve as reporters for our measurement system.</p>
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     <p><b>Reporter</b>: Our aim is the construction of an easy-to-use measurement system. Therefore we decided to use a reporter that delivers optical feedback which then can be quantified with an inexpensive photometer. Thus luciferase (LUC) serves as a reporter for our measurement system.</p>
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<p>In this weekly-journal you can <a href="/Team:Tuebingen/Notebook/Journal/Weekly">follow our overall progress</a>!</p>
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Latest revision as of 02:02, 5 October 2013

Return to iGEM Main Page.

Overview: Journal

Receptor: For simplification we denote the membranous progestin receptors (mPR) of Danio rerio and Xenopus laevis as "Receptor". One important property of the progestin receptor is its abilitiy to inhibit the FET3 gene promoter when progestin binds to the receptor. Since the Receptor serves as a sensor for progestins it is a very crucial element in our measurement system.

Inverter: The progestin receptors inhibit the promoter of the FET3 gene (Pfet3) when progestin is present in the cell's medium. In order to receive a positive feedback when progestin is detected we need to invert the receptor signal. Therefore we have combined Pfet3 with a repressor that represses the promotor of our reporter.

Reporter: Our aim is the construction of an easy-to-use measurement system. Therefore we decided to use a reporter that delivers optical feedback which then can be quantified with an inexpensive photometer. Thus luciferase (LUC) serves as a reporter for our measurement system.

In this weekly-journal you can follow our overall progress!