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Team:Tuebingen/Notebook/Protocols
From 2013.igem.org
(Difference between revisions)
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<li><a href="/Team:Tuebingen/Notebook/Protocols/gelextraction">Gel Extraction</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/gelextraction">Gel Extraction</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/colony-pcr">Colony-PCR</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/colony-pcr">Colony-PCR</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/tecanreader">Handling of the Tecan reader</a></li> | ||
</ul> | </ul> | ||
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<li><a href="/Team:Tuebingen/Notebook/Protocols/onestep">ONE-STEP buffer</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/onestep">ONE-STEP buffer</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/yeast-trafo">Yeast transformation</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/yeast-trafo">Yeast transformation</a></li> | ||
| - | |||
</ul> | </ul> | ||
Revision as of 03:51, 4 October 2013
Protocols
General and E. coli protocols
- SOB medium
- LB medium
- TAE buffer
- LB plates
- DNA-ladder-buffer mix
- Gelelectrophoresis
- Inoue buffer
- Chemo-competent E. coli cells
- E. coli transformation
- pGEM Ligaton - ligation for TA-cloning of PCR products
- Ligation of digested parts and vectors
- Glycerol stocks
- PCR
- 3A-Assembly
- Preparative restriction digest
- Control restriction digest
- Mini Prep
- Gel Extraction
- Colony-PCR
- Handling of the Tecan reader
Saccharomyces cerevisiae-specific protocols
