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Team:Tuebingen/Notebook/Protocols/lb
From 2013.igem.org
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<h3>Procedure</h3> | <h3>Procedure</h3> | ||
| - | <p>Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. Let cool down | + | <p>Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. Let cool down and add antibiotics if required. Store at 4 °C.</p> |
</div> | </div> | ||
Revision as of 02:54, 4 October 2013
LB Medium
Back to Protocols
Reagents
| 10.0 g | Trypton |
| 5.0 g | Yeast extract |
| 10.0 g | NaCl |
| Antibiotics |
Procedure
Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. Let cool down and add antibiotics if required. Store at 4 °C.
