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Team:Tuebingen/Notebook/Protocols/mini-prep
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Revision as of 12:05, 4 October 2013
Mini Prep
Procedure
- Inoculate 10 mL LB-medium with one single colony from a fresh plate (e.g. after transformation) and incubate at 37 °C and 220 rpm over night.
- On the next day, transfer culture to 15 mL Falcon tube and centrifuge for 10 min at 3500 rpm.
- Discard supernatant completely.
- Resuspend cells in 250 µL Genaxxon Buffer 1.5 (with RNase)
- Transfer cells to Eppendorf-tube.
- Continue according to Genaxxon Plasmid DNA Purification Mini Prep Kit Manual.
- In the end, elute with 30 µL Genaxxon Buffer 5.
- Store at -20 °C.
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