Team:Tuebingen/Notebook/Protocols/tecanreader
From 2013.igem.org
(Difference between revisions)
Line 16: | Line 16: | ||
<div id="actualContent"> | <div id="actualContent"> | ||
- | |||
- | <p> | + | <p>The Tecan reader is used for flourescence spectroscopy. Thus, tested cells should express some sort of flourescent protein. We mainly tested yeast cells that express mOrange - <a href="http://parts.igem.org/Part:BBa_E2050">BBa_E2050</a>.</p> |
<h3>Procedure</h3> | <h3>Procedure</h3> | ||
- | < | + | <ol> |
+ | <li>Inoculate 5 mL YPD medium with yeast cells. Create 1:10 and 1:100 dilutions of this liquid culture. Store all at 30°C.</li> | ||
+ | <li></li> | ||
+ | <li></li> | ||
+ | <li></li> | ||
+ | <li></li> | ||
+ | <li></li> | ||
+ | <li></li> | ||
+ | <li></li> | ||
+ | </ol> | ||
+ | <p> </p> | ||
+ | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | ||
</div> | </div> | ||
+ | |||
<!-- FOOTER --- IMPORTANT FOR NAVMENU-LENGTH!!! --> | <!-- FOOTER --- IMPORTANT FOR NAVMENU-LENGTH!!! --> |
Revision as of 19:55, 4 October 2013
Tecan Reader
The Tecan reader is used for flourescence spectroscopy. Thus, tested cells should express some sort of flourescent protein. We mainly tested yeast cells that express mOrange - BBa_E2050.
Procedure
- Inoculate 5 mL YPD medium with yeast cells. Create 1:10 and 1:100 dilutions of this liquid culture. Store all at 30°C.
Back to Protocols