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Team:Tuebingen/Project/Plasmids
From 2013.igem.org
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<p>We have designed our system to consist of three plasmids that will be be transformed into <i>S. cerevisiae</i>. Each plasmid will contain one part of our project - receptor, inverter, reporter - whereby there will be two receptor plasmids (one with mPR Dr, one with mPR Xl), two inverter plasmids (one with ROX1, one with MIG1), and two reporter plasmids (one with Psuc2, one with Panb1).</p> | <p>We have designed our system to consist of three plasmids that will be be transformed into <i>S. cerevisiae</i>. Each plasmid will contain one part of our project - receptor, inverter, reporter - whereby there will be two receptor plasmids (one with mPR Dr, one with mPR Xl), two inverter plasmids (one with ROX1, one with MIG1), and two reporter plasmids (one with Psuc2, one with Panb1).</p> | ||
| - | <p>Each plasmid will carry three parts, namely a promoter, a protein coding sequence, and the ADH1 terminator (Tadh1). We have retreived Tadh1 from the registry - <a href="http://parts.igem.org/Part:BBa_K801012">BBa_K801012</a></p> | + | <p>Each plasmid will carry three parts, namely a promoter, a protein coding sequence, and the ADH1 terminator (Tadh1). We have retreived Tadh1 from the registry - <a href="http://parts.igem.org/Part:BBa_K801012">BBa_K801012</a>.</p> |
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| + | <h3>"Receptor"-Plasmids</h3> | ||
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| + | <img src="https://static.igem.org/mediawiki/2012/d/dc/PRS313_Padh1_Danio_Tadh1.png" title="mPR Dr"> | ||
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| + | <img src"https://static.igem.org/mediawiki/2012/7/79/PRS313_Padh1_Xenopus_Tadh1.png" title="mPR Xl"> | ||
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Revision as of 15:54, 3 October 2013
Plasmids
We have designed our system to consist of three plasmids that will be be transformed into S. cerevisiae. Each plasmid will contain one part of our project - receptor, inverter, reporter - whereby there will be two receptor plasmids (one with mPR Dr, one with mPR Xl), two inverter plasmids (one with ROX1, one with MIG1), and two reporter plasmids (one with Psuc2, one with Panb1).
Each plasmid will carry three parts, namely a promoter, a protein coding sequence, and the ADH1 terminator (Tadh1). We have retreived Tadh1 from the registry - BBa_K801012.
"Receptor"-Plasmids
