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Team:Tuebingen/Project/Plasmids
From 2013.igem.org
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<h3>"Receptor"-Plasmids</h3> | <h3>"Receptor"-Plasmids</h3> | ||
<p>pRS313 containing Padh1, mPR Dr, and Tadh1:</p> | <p>pRS313 containing Padh1, mPR Dr, and Tadh1:</p> | ||
| - | <img src="https://static.igem.org/mediawiki/2012/d/dc/PRS313_Padh1_Danio_Tadh1.png" title="mPR Dr"> | + | <img src="https://static.igem.org/mediawiki/2012/d/dc/PRS313_Padh1_Danio_Tadh1.png" title="Padh1-mPR Dr-Tadh1 pRS313"> |
<div class="verticallyPlease"></div> | <div class="verticallyPlease"></div> | ||
<p>pRS313 containing Padh1, mPR Xl, and Tadh1:</p> | <p>pRS313 containing Padh1, mPR Xl, and Tadh1:</p> | ||
| - | <img src="https://static.igem.org/mediawiki/2012/7/79/PRS313_Padh1_Xenopus_Tadh1.png" title="mPR Xl"> | + | <img src="https://static.igem.org/mediawiki/2012/7/79/PRS313_Padh1_Xenopus_Tadh1.png" title="Padh1-mPR Xl-Tadh1 pRS313"> |
<div class="verticallyPlease"></div> | <div class="verticallyPlease"></div> | ||
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<h3>"Inverter"-Plasmids</h3> | <h3>"Inverter"-Plasmids</h3> | ||
<p>pRS315 containing Pfet3, rox1, and Tadh1:</p> | <p>pRS315 containing Pfet3, rox1, and Tadh1:</p> | ||
| - | <img src="https://static.igem.org/mediawiki/2012/9/94/PRS315_Pfet3_rox1_Tadh1_V3.png" title="rox1"> | + | <img src="https://static.igem.org/mediawiki/2012/9/94/PRS315_Pfet3_rox1_Tadh1_V3.png" title="Pfet3-rox1-Tadh1 pRS315"> |
<div class="verticallyPlease"></div> | <div class="verticallyPlease"></div> | ||
<p>pRS315 containing Pfet3, mig1, and Tadh1:</p> | <p>pRS315 containing Pfet3, mig1, and Tadh1:</p> | ||
| - | <img src="https://static.igem.org/mediawiki/2012/0/0c/PRS315_Pfet3_mig1_Tadh1.png" title="mig1"> | + | <img src="https://static.igem.org/mediawiki/2012/0/0c/PRS315_Pfet3_mig1_Tadh1.png" title="Pfet3-mig1-Tadh1 pRS315"> |
<div class="verticallyPlease"></div> | <div class="verticallyPlease"></div> | ||
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<h3>"Reporter"-Plasmids</h3> | <h3>"Reporter"-Plasmids</h3> | ||
<p>pRS316 containing Panb1, luciferase (luc), and Tadh1:</p> | <p>pRS316 containing Panb1, luciferase (luc), and Tadh1:</p> | ||
| - | <img src="https://static.igem.org/mediawiki/2012/9/9e/PRS316_Psuc2_Luciferase_Tadh1.png" title=" | + | <img src="https://static.igem.org/mediawiki/2012/9/9e/PRS316_Psuc2_Luciferase_Tadh1.png" title="Panb1-Luciferase-Tadh pRS316"> |
<div class="verticallyPlease"></div> | <div class="verticallyPlease"></div> | ||
<p>pRS316 containing Psuc2, luciferase (luc), and Tadh1:</p> | <p>pRS316 containing Psuc2, luciferase (luc), and Tadh1:</p> | ||
| - | <img src="https://static.igem.org/mediawiki/2012/9/9e/PRS316_Psuc2_Luciferase_Tadh1.png" title=" | + | <img src="https://static.igem.org/mediawiki/2012/9/9e/PRS316_Psuc2_Luciferase_Tadh1.png" title="Panb1-Luciferase-Tadh pRS316"> |
<div class="verticallyPlease"></div> | <div class="verticallyPlease"></div> | ||
Revision as of 16:40, 3 October 2013
We have designed our system to consist of three plasmids that will be be transformed into S. cerevisiae. Each plasmid will contain one part of our project - receptor, inverter, reporter - whereby there will be two receptor plasmids (one with mPR Dr, one with mPR Xl), two inverter plasmids (one with ROX1, one with MIG1), and two reporter plasmids (one with Psuc2, one with Panb1).
Each plasmid will carry three parts, namely a promoter, a protein coding sequence, and the ADH1 terminator (Tadh1). We have retreived Tadh1 from the registry - BBa_K801012.
"Receptor"-Plasmids
pRS313 containing Padh1, mPR Dr, and Tadh1:
pRS313 containing Padh1, mPR Xl, and Tadh1:
"Inverter"-Plasmids
pRS315 containing Pfet3, rox1, and Tadh1:
pRS315 containing Pfet3, mig1, and Tadh1:
"Reporter"-Plasmids
pRS316 containing Panb1, luciferase (luc), and Tadh1:
pRS316 containing Psuc2, luciferase (luc), and Tadh1:
