Team:USTC CHINA/Notebook/Protocols/Expression of proteins in Bacillus subtilis

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<h1>Expression of proteins in Bacillus subtilis</h1>
<h1>Expression of proteins in Bacillus subtilis</h1>
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<p>Induction with IPTG
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<p><span>Induction with IPTG</span></br>
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- grow appropriate B. subtilis strain overnight in fresh 2xYT medium
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- grow appropriate B. subtilis strain overnight in fresh 2xYT medium</br>
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- inoculate into fresh 2xYT medium to an OD600 of 0.15
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- inoculate into fresh 2xYT medium to an OD600 of 0.15</br>
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- when culture reaches OD600 0.7 – 0.8, split into 2 portions and induce with 1 mM IPTG
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- when culture reaches OD600 0.7 – 0.8, split into 2 portions and induce with 1 mM </br>
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(CB-0487-1GAM) to one portion (t = 0)
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  IPTG(CB-0487-1GAM) to one portion (t = 0)</br>
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- collect samples at different time points for analysis (t = 1,…)
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- collect samples at different time points for analysis (t = 1,…)</br>
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Latest revision as of 09:01, 27 September 2013

Expression of proteins in Bacillus subtilis

Induction with IPTG
- grow appropriate B. subtilis strain overnight in fresh 2xYT medium
- inoculate into fresh 2xYT medium to an OD600 of 0.15
- when culture reaches OD600 0.7 – 0.8, split into 2 portions and induce with 1 mM
IPTG(CB-0487-1GAM) to one portion (t = 0)
- collect samples at different time points for analysis (t = 1,…)