Template:Kyoto/Notebook/Aug 29

From 2013.igem.org

(Difference between revisions)
(→Aug 29)
(→miniprep)
Line 47: Line 47:
</div>
</div>
===miniprep===
===miniprep===
 +
<div class="experiment">
 +
<span class="author">No Name</span>
 +
{|class="wikitable"
 +
!DNA||concentration [&micro;g/mL]||260/280||260/230
 +
|-
 +
|Plac||153.7||2.03||2.10
 +
|-
 +
|Pλ-luxⅠ-(1)||76.5||2.07||2.06
 +
|-
 +
|Pλ-luxⅠ-(2)||86.4||2.08||2.10
 +
|-
 +
|DT||166.2||1.65||2.12
 +
|-
 +
|J23100||190.1||1.68||2.34
 +
|}
 +
</div>
 +
===Liquid Culture===
===Liquid Culture===
===Restriction Enzyme Digestion===
===Restriction Enzyme Digestion===
===Transformation===
===Transformation===
===M9 Medium Plate===
===M9 Medium Plate===

Revision as of 10:03, 21 September 2013

Contents

Aug 29

Colony PCR

No name

Samplebase pair
8/27 Pcon-pT181 antisense-(1)542
8/26 pT181 attenuator-DT-(1)738
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s30s30cycles

File:Igku Aug19electrophoresis1.png

Electrophoresis

No name

LaneSample
1100bp ladder
28/21 RBS-lysis2-9(Colony PCR prodution)
38/21 RBS-lysis2-8(Colony PCR prodution)
48/21 RBS-lysis2-7(Colony PCR prodution)
58/21 RBS-lysis2-10(Colony PCR prodution)
68/21 RBS-lysis2-11(Colony PCR prodution)
78/21 RBS-lysis2-12(Colony PCR prodution)
88/21 RBS-lysis2-13(Colony PCR prodution)
98/21 RBS-lysis2-14(Colony PCR prodution)

File:Igku xxxxxx.xxx

miniprep

No Name

DNAconcentration [µg/mL]260/280260/230
Plac153.72.032.10
Pλ-luxⅠ-(1)76.52.072.06
Pλ-luxⅠ-(2)86.42.082.10
DT166.21.652.12
J23100190.11.682.34

Liquid Culture

Restriction Enzyme Digestion

Transformation

M9 Medium Plate

Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Aug_29"