From 2013.igem.org

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Gel electrophoresis

  • Two different sets of gel were run due to the different fragment size of the PCR product.
  • Smaller fragments were run on a 2% agarose gel whilst larger fragments were run on a 1% gel.

DNA extraction from gel

  • Extracting previously run DNA from gel using Thermo Scientific GeneJet gel extraction kit and following its protocol.
  • Concentrations of DNA were measured by nanodrop
  • Sample B (Tod C gene) was not purified correctly, another PCR is needed. (Nanodrop shows contamination)
  • Table representing the concentration and volume of DNA isolated from samples
SampleVolumeConcentration ng/ul260/280260/230