"

From 2013.igem.org

5/20:
Group A:
Overnight culture of pGAPzα A, B, C, and A modifid.
Group B:
Miniprep of pGAPzα B.
Group C:
Eight hour cultures of E. coli + RFP.
Midiprep of E. coli + PGAPzα C.
Overnight culture of P. pastoris.
Digest of pGAPzα C with EcoRI and XbaI.